Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize

Over a period of 5 years, there was conducted a series of trials on the use of γ-irradiation of the female gametophyte of spring common wheat before remote hybridization with maize to study the effect of the phytohormonal changes during irradiation at various doses on ovaries’ growth after pollinati...

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Main Authors: E. V. Bondarenko, I. F. Lapochkina, I. A. Pishenin, I. Yu. Makarova, N. A. Yashina, A. S. Smirnova, E. A. Kazakova, Ya. A. Blinova, A. A. Prazyan, P. Yu. Volkova
Format: Article
Language:Russian
Published: Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”" 2024-09-01
Series:Зерновое хозяйство России
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Online Access:https://www.zhros.online/jour/article/view/2763
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author E. V. Bondarenko
I. F. Lapochkina
I. A. Pishenin
I. Yu. Makarova
N. A. Yashina
A. S. Smirnova
E. A. Kazakova
Ya. A. Blinova
A. A. Prazyan
P. Yu. Volkova
author_facet E. V. Bondarenko
I. F. Lapochkina
I. A. Pishenin
I. Yu. Makarova
N. A. Yashina
A. S. Smirnova
E. A. Kazakova
Ya. A. Blinova
A. A. Prazyan
P. Yu. Volkova
author_sort E. V. Bondarenko
collection DOAJ
description Over a period of 5 years, there was conducted a series of trials on the use of γ-irradiation of the female gametophyte of spring common wheat before remote hybridization with maize to study the effect of the phytohormonal changes during irradiation at various doses on ovaries’ growth after pollination with maize. Optimal timing of work on castration, irradiation, and pollination with maize pollen were established. It has been concluded that it is necessary to conduct such experiments in controlled conditions of a climatic chamber to avoid additional stress during fertilization and development of an embryo. The best results in ovaries’ growth were obtained (7.89–11.30 % of developed ovaries) with γ-irradiation in the dose from 3 to 15 Gy. There have been found changes in the level of various phytohormones and the possible influence of these changes on ovaries’ setting and growth, as potential sources of haploid and apomictically developed genotypes. The variants with the best indicators of developed ovaries are characterized by an increase in the ratio values of the salicylic acid/indole-3-acetic acid and salicylic acid/abscisic acid in relation to the same values before irradiation.
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institution Kabale University
issn 2079-8725
2079-8733
language Russian
publishDate 2024-09-01
publisher Federal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"
record_format Article
series Зерновое хозяйство России
spelling doaj-art-0a54971a8e4749aa8207eb4146df96642025-08-20T03:57:36ZrusFederal State Budgetary Scientific Institution “Agricultural Research Center “Donskoy”"Зерновое хозяйство России2079-87252079-87332024-09-01164505910.31367/2079-8725-2024-93-4-50-59965Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maizeE. V. Bondarenko0I. F. Lapochkina1I. A. Pishenin2I. Yu. Makarova3N. A. Yashina4A. S. Smirnova5E. A. Kazakova6Ya. A. Blinova7A. A. Prazyan8P. Yu. VolkovaFederal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)Federal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)FSBSI “Federal Research Center “Nemchinovka” (FRC “Nemchinovka”)Federal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”Federal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”Federal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”Federal State Budgetary Institution “All-Russian Research Institute of Radiology and Agroecology of the National Research Center “Kurchatov Institute”Over a period of 5 years, there was conducted a series of trials on the use of γ-irradiation of the female gametophyte of spring common wheat before remote hybridization with maize to study the effect of the phytohormonal changes during irradiation at various doses on ovaries’ growth after pollination with maize. Optimal timing of work on castration, irradiation, and pollination with maize pollen were established. It has been concluded that it is necessary to conduct such experiments in controlled conditions of a climatic chamber to avoid additional stress during fertilization and development of an embryo. The best results in ovaries’ growth were obtained (7.89–11.30 % of developed ovaries) with γ-irradiation in the dose from 3 to 15 Gy. There have been found changes in the level of various phytohormones and the possible influence of these changes on ovaries’ setting and growth, as potential sources of haploid and apomictically developed genotypes. The variants with the best indicators of developed ovaries are characterized by an increase in the ratio values of the salicylic acid/indole-3-acetic acid and salicylic acid/abscisic acid in relation to the same values before irradiation.https://www.zhros.online/jour/article/view/2763common wheatmaizegamma irradiationphytohormonesfemale gametophyteovaries
spellingShingle E. V. Bondarenko
I. F. Lapochkina
I. A. Pishenin
I. Yu. Makarova
N. A. Yashina
A. S. Smirnova
E. A. Kazakova
Ya. A. Blinova
A. A. Prazyan
P. Yu. Volkova
Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
Зерновое хозяйство России
common wheat
maize
gamma irradiation
phytohormones
female gametophyte
ovaries
title Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
title_full Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
title_fullStr Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
title_full_unstemmed Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
title_short Gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries’ growth when crossed with maize
title_sort gamma irradiation effect of female gametophyte in emasculated spring bread wheat plants on ovaries growth when crossed with maize
topic common wheat
maize
gamma irradiation
phytohormones
female gametophyte
ovaries
url https://www.zhros.online/jour/article/view/2763
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