Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease
The aim of the present study was to gain insight into the neuroprotection effects and mechanism of thiazolidinedione pioglitazone in both in vitro and in vivo MPP+/MPTP induced PD models. In vivo experimental results showed that oral treatment of pioglitazone resulted in significant improvements in...
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| Format: | Article |
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Wiley
2017-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2017/4089214 |
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| author | Yanqin Wang Weilin Zhao Ge Li Jinhu Chen Xin Guan Xi Chen Zhenlong Guan |
| author_facet | Yanqin Wang Weilin Zhao Ge Li Jinhu Chen Xin Guan Xi Chen Zhenlong Guan |
| author_sort | Yanqin Wang |
| collection | DOAJ |
| description | The aim of the present study was to gain insight into the neuroprotection effects and mechanism of thiazolidinedione pioglitazone in both in vitro and in vivo MPP+/MPTP induced PD models. In vivo experimental results showed that oral treatment of pioglitazone resulted in significant improvements in behavior symptoms damaged by MPTP and increase in the survival of TH positive neurons in the pioglitazone intervention groups. In addition, oral treatment of pioglitazone increased the expression of peroxisome proliferator-activated receptor-γ coactivator of 1α (PGC-1α) and increased the number of mitochondria, along with an observed improvement in mitochondrial ultrastructure. From in vitro studies, 2,4-thiazolidinedione resulted in increased levels of molecules regulated function of mitochondria, including PGC-1α, nuclear respiratory factor 1 (NRF1), NRF2, and mitochondria fusion 2 (Mfn2), and inhibited mitochondria fission 1 (Fis1). We show that protein levels of Bcl-2 and ERK were reduced in the MPP+-treated group compared with the control group. This effect was observed to be reversed upon treatment with 2,4-thiazolidinedione, as Bcl-2 and ERK expression levels were increased. We also observed that levels of the apoptotic protein Bax showed opposite changes compared to Bcl-2 and ERK levels. The results from this study confirm that pioglitazone/2,4-thiazolidinedione is able to activate PGC-1α and prevent damage of dopaminergic neurons and restore mitochondria ultrastructure through the regulation of mitochondria function. |
| format | Article |
| id | doaj-art-0a3bba58f68047cfbcf9ed2b00413185 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
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| series | PPAR Research |
| spelling | doaj-art-0a3bba58f68047cfbcf9ed2b004131852025-08-20T03:55:27ZengWileyPPAR Research1687-47571687-47652017-01-01201710.1155/2017/40892144089214Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s DiseaseYanqin Wang0Weilin Zhao1Ge Li2Jinhu Chen3Xin Guan4Xi Chen5Zhenlong Guan6Department of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, ChinaHuman Movement Science, Hebei Institute of Physical Education, Shijiazhuang, Hebei 050041, ChinaDepartment of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, ChinaDepartment of Endocrinology, Hebei General Hospital, Shijiazhuang, Hebei 050051, ChinaDepartment of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, ChinaDepartment of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, ChinaDepartment of Physiology, College of Life Science, Hebei Normal University, Shijiazhuang, Hebei 050024, ChinaThe aim of the present study was to gain insight into the neuroprotection effects and mechanism of thiazolidinedione pioglitazone in both in vitro and in vivo MPP+/MPTP induced PD models. In vivo experimental results showed that oral treatment of pioglitazone resulted in significant improvements in behavior symptoms damaged by MPTP and increase in the survival of TH positive neurons in the pioglitazone intervention groups. In addition, oral treatment of pioglitazone increased the expression of peroxisome proliferator-activated receptor-γ coactivator of 1α (PGC-1α) and increased the number of mitochondria, along with an observed improvement in mitochondrial ultrastructure. From in vitro studies, 2,4-thiazolidinedione resulted in increased levels of molecules regulated function of mitochondria, including PGC-1α, nuclear respiratory factor 1 (NRF1), NRF2, and mitochondria fusion 2 (Mfn2), and inhibited mitochondria fission 1 (Fis1). We show that protein levels of Bcl-2 and ERK were reduced in the MPP+-treated group compared with the control group. This effect was observed to be reversed upon treatment with 2,4-thiazolidinedione, as Bcl-2 and ERK expression levels were increased. We also observed that levels of the apoptotic protein Bax showed opposite changes compared to Bcl-2 and ERK levels. The results from this study confirm that pioglitazone/2,4-thiazolidinedione is able to activate PGC-1α and prevent damage of dopaminergic neurons and restore mitochondria ultrastructure through the regulation of mitochondria function.http://dx.doi.org/10.1155/2017/4089214 |
| spellingShingle | Yanqin Wang Weilin Zhao Ge Li Jinhu Chen Xin Guan Xi Chen Zhenlong Guan Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease PPAR Research |
| title | Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease |
| title_full | Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease |
| title_fullStr | Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease |
| title_full_unstemmed | Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease |
| title_short | Neuroprotective Effect and Mechanism of Thiazolidinedione on Dopaminergic Neurons In Vivo and In Vitro in Parkinson’s Disease |
| title_sort | neuroprotective effect and mechanism of thiazolidinedione on dopaminergic neurons in vivo and in vitro in parkinson s disease |
| url | http://dx.doi.org/10.1155/2017/4089214 |
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