Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i>
This study aimed to explore the role of the <i>trehalose-6-phosphate synthase</i> (<i>TPS</i>) gene family in the adaptation of peas to environmental stress. A comprehensive analysis of the <i>PsTPS</i> gene family identified 20 genes with conserved domains and sp...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-10-01
|
| Series: | Horticulturae |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-7524/10/10/1104 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850205183863160832 |
|---|---|
| author | Hao Yuan Baoxia Liu Guwen Zhang Zhijuan Feng Bin Wang Yuanpeng Bu Yu Xu Zhihong Sun Na Liu Yaming Gong |
| author_facet | Hao Yuan Baoxia Liu Guwen Zhang Zhijuan Feng Bin Wang Yuanpeng Bu Yu Xu Zhihong Sun Na Liu Yaming Gong |
| author_sort | Hao Yuan |
| collection | DOAJ |
| description | This study aimed to explore the role of the <i>trehalose-6-phosphate synthase</i> (<i>TPS</i>) gene family in the adaptation of peas to environmental stress. A comprehensive analysis of the <i>PsTPS</i> gene family identified 20 genes with conserved domains and specific chromosomal locations. Phylogenetic analysis delineated evolutionary relationships, while gene structure analysis revealed compositional insights, and motif analysis provided functional insights. Cis-regulatory element identification predicted gene regulation patterns. Tissue-specific and stress-induced expression profiling highlighted eight genes with ubiquitous expression, with <i>PsTPS15</i> and <i>PsTPS18</i> displaying elevated expression levels in roots, nodules, and young stems, and <i>PsTPS13</i> and <i>PsTPS19</i> expression downregulated in seeds. Transcriptome analysis identified a differential expression of 20 <i>PsTPS</i> genes, highlighting the significance of 14 genes in response to drought and salinity stress. Notably, under drought conditions, the expression of <i>PsTPS4</i> and <i>PsTPS6</i> was initially upregulated and then downregulated, whereas that of <i>PsTPS15</i> and <i>PsTPS19</i> was downregulated. Salinity stress notably altered the expression of <i>PsTPS4</i>, <i>PsTPS6</i>, and <i>PsTPS19</i>. Taken together, these findings elucidate the regulatory mechanisms of the <i>PsTPS</i> gene family and their potential as genetic targets for enhancing crop stress tolerance. |
| format | Article |
| id | doaj-art-0a306f43a21843b5bf0d1766d2edb1e0 |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-0a306f43a21843b5bf0d1766d2edb1e02025-08-20T02:11:09ZengMDPI AGHorticulturae2311-75242024-10-011010110410.3390/horticulturae10101104Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i>Hao Yuan0Baoxia Liu1Guwen Zhang2Zhijuan Feng3Bin Wang4Yuanpeng Bu5Yu Xu6Zhihong Sun7Na Liu8Yaming Gong9State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaCollege of Horticulture Science, Zhejiang A&F University, Hangzhou 311300, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaState Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Ministry of Agriculture and Rural Affairs Key Laboratory of Vegetable Legumes Germplasm Enhancement and Molecular Breeding in Southern China, Institute of Vegetables, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, ChinaThis study aimed to explore the role of the <i>trehalose-6-phosphate synthase</i> (<i>TPS</i>) gene family in the adaptation of peas to environmental stress. A comprehensive analysis of the <i>PsTPS</i> gene family identified 20 genes with conserved domains and specific chromosomal locations. Phylogenetic analysis delineated evolutionary relationships, while gene structure analysis revealed compositional insights, and motif analysis provided functional insights. Cis-regulatory element identification predicted gene regulation patterns. Tissue-specific and stress-induced expression profiling highlighted eight genes with ubiquitous expression, with <i>PsTPS15</i> and <i>PsTPS18</i> displaying elevated expression levels in roots, nodules, and young stems, and <i>PsTPS13</i> and <i>PsTPS19</i> expression downregulated in seeds. Transcriptome analysis identified a differential expression of 20 <i>PsTPS</i> genes, highlighting the significance of 14 genes in response to drought and salinity stress. Notably, under drought conditions, the expression of <i>PsTPS4</i> and <i>PsTPS6</i> was initially upregulated and then downregulated, whereas that of <i>PsTPS15</i> and <i>PsTPS19</i> was downregulated. Salinity stress notably altered the expression of <i>PsTPS4</i>, <i>PsTPS6</i>, and <i>PsTPS19</i>. Taken together, these findings elucidate the regulatory mechanisms of the <i>PsTPS</i> gene family and their potential as genetic targets for enhancing crop stress tolerance.https://www.mdpi.com/2311-7524/10/10/1104<i>Pisum sativum</i><i>PsTPS</i>evolutionary analysesgene expressiondrought stresssalt stress |
| spellingShingle | Hao Yuan Baoxia Liu Guwen Zhang Zhijuan Feng Bin Wang Yuanpeng Bu Yu Xu Zhihong Sun Na Liu Yaming Gong Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> Horticulturae <i>Pisum sativum</i> <i>PsTPS</i> evolutionary analyses gene expression drought stress salt stress |
| title | Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> |
| title_full | Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> |
| title_fullStr | Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> |
| title_short | Genome-Wide Identification and Expression Analysis of the <i>PsTPS</i> Gene Family in <i>Pisum sativum</i> |
| title_sort | genome wide identification and expression analysis of the i pstps i gene family in i pisum sativum i |
| topic | <i>Pisum sativum</i> <i>PsTPS</i> evolutionary analyses gene expression drought stress salt stress |
| url | https://www.mdpi.com/2311-7524/10/10/1104 |
| work_keys_str_mv | AT haoyuan genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT baoxialiu genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT guwenzhang genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT zhijuanfeng genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT binwang genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT yuanpengbu genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT yuxu genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT zhihongsun genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT naliu genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi AT yaminggong genomewideidentificationandexpressionanalysisoftheipstpsigenefamilyinipisumsativumi |