Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia
Waldenstrom macroglobulinemia is a lymphoplasmacytic lymphoma, the morphological substrates of which are b‑lymphocytes, proplasmocytes, and plasma cells. The world Health Organization recommends multicolor flow cytometry with analysis of markers such as IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103...
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| Language: | Russian |
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ABV-press
2025-03-01
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| Series: | Онкогематология |
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| Online Access: | https://oncohematology.abvpress.ru/ongm/article/view/1012 |
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| author | I. V. Galtseva Yu. A. Tsoy A. E. Grachev N. M. Kapranov K. A. Nikiforova Yu. O. Davydova A. A. Kulikov E. E. Zvonkov E. N. Parovichnikova |
| author_facet | I. V. Galtseva Yu. A. Tsoy A. E. Grachev N. M. Kapranov K. A. Nikiforova Yu. O. Davydova A. A. Kulikov E. E. Zvonkov E. N. Parovichnikova |
| author_sort | I. V. Galtseva |
| collection | DOAJ |
| description | Waldenstrom macroglobulinemia is a lymphoplasmacytic lymphoma, the morphological substrates of which are b‑lymphocytes, proplasmocytes, and plasma cells. The world Health Organization recommends multicolor flow cytometry with analysis of markers such as IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103, Cd138, for diagnosing this disease. Based on international and our own experience, we recommend that tumor b‑lymphocytes and plasma cells be analyzed separately for the diagnosis of waldenstrom macroglobulinemia, since the immunophenotypic profile of these populations differs. In diagnostics, this approach provides a more complete understanding of various subpopulations contribution, and when monitoring minimal residual disease, it helps to detect the tumor clone, which after therapy is predominantly represented by plasma cells. we recommend using antibodies to surface and intracellular markers such as Cd138, Cd38, Cd19, Cd45, Cd20, Cd22, Cd27 cytκ, cytλ and cytIgM for immunophenotypic testing of waldenstrom macroglobulinemia. |
| format | Article |
| id | doaj-art-0a2ea64ac281490b9b2b2c6b416ce764 |
| institution | DOAJ |
| issn | 1818-8346 2413-4023 |
| language | Russian |
| publishDate | 2025-03-01 |
| publisher | ABV-press |
| record_format | Article |
| series | Онкогематология |
| spelling | doaj-art-0a2ea64ac281490b9b2b2c6b416ce7642025-08-20T03:21:28ZrusABV-pressОнкогематология1818-83462413-40232025-03-0120112813810.17650/1818-8346-2025-20-1-128-138814Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemiaI. V. Galtseva0Yu. A. Tsoy1A. E. Grachev2N. M. Kapranov3K. A. Nikiforova4Yu. O. Davydova5A. A. Kulikov6E. E. Zvonkov7E. N. Parovichnikova8National Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaNational Medical Research Center for Hematology, Ministry of Health of RussiaWaldenstrom macroglobulinemia is a lymphoplasmacytic lymphoma, the morphological substrates of which are b‑lymphocytes, proplasmocytes, and plasma cells. The world Health Organization recommends multicolor flow cytometry with analysis of markers such as IgM, Cd19, Cd20, Cd22, Cd25, Cd10, Cd23, Cd103, Cd138, for diagnosing this disease. Based on international and our own experience, we recommend that tumor b‑lymphocytes and plasma cells be analyzed separately for the diagnosis of waldenstrom macroglobulinemia, since the immunophenotypic profile of these populations differs. In diagnostics, this approach provides a more complete understanding of various subpopulations contribution, and when monitoring minimal residual disease, it helps to detect the tumor clone, which after therapy is predominantly represented by plasma cells. we recommend using antibodies to surface and intracellular markers such as Cd138, Cd38, Cd19, Cd45, Cd20, Cd22, Cd27 cytκ, cytλ and cytIgM for immunophenotypic testing of waldenstrom macroglobulinemia.https://oncohematology.abvpress.ru/ongm/article/view/1012waldenstrom macroglobulinemialymphoplasmacytic lymphomamulticolor flow cytometryb‑lymphocytesplasma cells |
| spellingShingle | I. V. Galtseva Yu. A. Tsoy A. E. Grachev N. M. Kapranov K. A. Nikiforova Yu. O. Davydova A. A. Kulikov E. E. Zvonkov E. N. Parovichnikova Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia Онкогематология waldenstrom macroglobulinemia lymphoplasmacytic lymphoma multicolor flow cytometry b‑lymphocytes plasma cells |
| title | Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia |
| title_full | Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia |
| title_fullStr | Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia |
| title_full_unstemmed | Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia |
| title_short | Multicolor flow cytometry in the diagnosis of Waldenstrom macroglobulinemia |
| title_sort | multicolor flow cytometry in the diagnosis of waldenstrom macroglobulinemia |
| topic | waldenstrom macroglobulinemia lymphoplasmacytic lymphoma multicolor flow cytometry b‑lymphocytes plasma cells |
| url | https://oncohematology.abvpress.ru/ongm/article/view/1012 |
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