TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA
Thousands of people suffer from severe malaria every year. The innate immune response plays a determinant role in host’s defence to malaria. Transient receptor potential vanilloid 1 (TRPV1) modulates macrophage-mediated responses in sepsis, but its role in other pathogenic diseases has never been ad...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/506450 |
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| author | Elizabeth S. Fernandes Carolina X. L. Brito Simone A. Teixeira Renato Barboza Aramys S. dos Reis Ana Paula S. Azevedo-Santos Marcelo Muscará Soraia K. P. Costa Claudio R. F. Marinho Susan D. Brain Marcos A. G. Grisotto |
| author_facet | Elizabeth S. Fernandes Carolina X. L. Brito Simone A. Teixeira Renato Barboza Aramys S. dos Reis Ana Paula S. Azevedo-Santos Marcelo Muscará Soraia K. P. Costa Claudio R. F. Marinho Susan D. Brain Marcos A. G. Grisotto |
| author_sort | Elizabeth S. Fernandes |
| collection | DOAJ |
| description | Thousands of people suffer from severe malaria every year. The innate immune response plays a determinant role in host’s defence to malaria. Transient receptor potential vanilloid 1 (TRPV1) modulates macrophage-mediated responses in sepsis, but its role in other pathogenic diseases has never been addressed. We investigated the effects of capsazepine, a TRPV1 antagonist, in malaria. C57BL/6 mice received 105 red blood cells infected with Plasmodium berghei ANKA intraperitoneally. Noninfected mice were used as controls. Capsazepine or vehicle was given intraperitoneally for 6 days. Mice were culled on day 7 after infection and blood and spleen cell phenotype and activation were evaluated. Capsazepine decreased circulating but not spleen F4/80+Ly6G+ cell numbers as well as activation of both F4/80+and F4/80+Ly6G+ cells in infected animals. In addition, capsazepine increased circulating but not spleen GR1+ and natural killer (NK) population, without interfering with natural killer T (NKT) cell numbers and blood NK and NKT activation. However, capsazepine diminished CD69 expression in spleen NKT but not NK cells. Infection increased lipid peroxidation and the release of TNFα and IFNγ, although capsazepine-treated group exhibited lower levels of lipid peroxidation and TNFα. Capsazepine treatment did not affect parasitaemia. Overall, TRPV1 antagonism modulates the innate immune response to malaria. |
| format | Article |
| id | doaj-art-0a204f7dd7414927b03ee7a8dd4dc960 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-0a204f7dd7414927b03ee7a8dd4dc9602025-02-03T05:51:54ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/506450506450TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKAElizabeth S. Fernandes0Carolina X. L. Brito1Simone A. Teixeira2Renato Barboza3Aramys S. dos Reis4Ana Paula S. Azevedo-Santos5Marcelo Muscará6Soraia K. P. Costa7Claudio R. F. Marinho8Susan D. Brain9Marcos A. G. Grisotto10Universidade CEUMA, 65075-120 São Luís, MA, BrazilUniversidade CEUMA, 65075-120 São Luís, MA, BrazilUniversidade de São Paulo, São Paulo, BrazilUniversidade Federal de São Paulo, Diadema, BrazilUniversidade de São Paulo, São Paulo, BrazilUniversidade Federal do Maranhão, São Luís, BrazilUniversidade de São Paulo, São Paulo, BrazilUniversidade de São Paulo, São Paulo, BrazilUniversidade de São Paulo, São Paulo, BrazilCardiovascular Division, King’s College London, London, UKUniversidade CEUMA, 65075-120 São Luís, MA, BrazilThousands of people suffer from severe malaria every year. The innate immune response plays a determinant role in host’s defence to malaria. Transient receptor potential vanilloid 1 (TRPV1) modulates macrophage-mediated responses in sepsis, but its role in other pathogenic diseases has never been addressed. We investigated the effects of capsazepine, a TRPV1 antagonist, in malaria. C57BL/6 mice received 105 red blood cells infected with Plasmodium berghei ANKA intraperitoneally. Noninfected mice were used as controls. Capsazepine or vehicle was given intraperitoneally for 6 days. Mice were culled on day 7 after infection and blood and spleen cell phenotype and activation were evaluated. Capsazepine decreased circulating but not spleen F4/80+Ly6G+ cell numbers as well as activation of both F4/80+and F4/80+Ly6G+ cells in infected animals. In addition, capsazepine increased circulating but not spleen GR1+ and natural killer (NK) population, without interfering with natural killer T (NKT) cell numbers and blood NK and NKT activation. However, capsazepine diminished CD69 expression in spleen NKT but not NK cells. Infection increased lipid peroxidation and the release of TNFα and IFNγ, although capsazepine-treated group exhibited lower levels of lipid peroxidation and TNFα. Capsazepine treatment did not affect parasitaemia. Overall, TRPV1 antagonism modulates the innate immune response to malaria.http://dx.doi.org/10.1155/2014/506450 |
| spellingShingle | Elizabeth S. Fernandes Carolina X. L. Brito Simone A. Teixeira Renato Barboza Aramys S. dos Reis Ana Paula S. Azevedo-Santos Marcelo Muscará Soraia K. P. Costa Claudio R. F. Marinho Susan D. Brain Marcos A. G. Grisotto TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA Mediators of Inflammation |
| title | TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA |
| title_full | TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA |
| title_fullStr | TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA |
| title_full_unstemmed | TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA |
| title_short | TRPV1 Antagonism by Capsazepine Modulates Innate Immune Response in Mice Infected with Plasmodium berghei ANKA |
| title_sort | trpv1 antagonism by capsazepine modulates innate immune response in mice infected with plasmodium berghei anka |
| url | http://dx.doi.org/10.1155/2014/506450 |
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