Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination
Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•−) which ra...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | International Journal of Analytical Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/109592 |
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| author | Muhammad Asgher Mohammad Yaqoob Abdul Nabi Ghulam Murtaza Abdul Rauf Siddiqi Amir Waseem |
| author_facet | Muhammad Asgher Mohammad Yaqoob Abdul Nabi Ghulam Murtaza Abdul Rauf Siddiqi Amir Waseem |
| author_sort | Muhammad Asgher |
| collection | DOAJ |
| description | Rose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•−) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•−) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals (•OH) were produced via dismutation of H2O2 by catalyst (Cu2+). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n=6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n=3) in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values. |
| format | Article |
| id | doaj-art-0a102baa5faa42da8e522633df3e0640 |
| institution | Kabale University |
| issn | 1687-8760 1687-8779 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | International Journal of Analytical Chemistry |
| spelling | doaj-art-0a102baa5faa42da8e522633df3e06402025-08-20T03:55:27ZengWileyInternational Journal of Analytical Chemistry1687-87601687-87792014-01-01201410.1155/2014/109592109592Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C DeterminationMuhammad Asgher0Mohammad Yaqoob1Abdul Nabi2Ghulam Murtaza3Abdul Rauf Siddiqi4Amir Waseem5Department of Chemistry, University of Balochistan, Sariab Road, Quetta 87300, PakistanDepartment of Chemistry, University of Balochistan, Sariab Road, Quetta 87300, PakistanDepartment of Chemistry, University of Balochistan, Sariab Road, Quetta 87300, PakistanDepartment of Pharmacy, COMSATS Institute of Information Technology, Abbottabad 22060, PakistanDepartment of Biosciences, COMSATS Institute of Information Technology, Islamabad 45320, PakistanDepartment of Chemistry, Quaid-i-Azam University, Islamabad 45320, PakistanRose Bengal photosensitized flow injection chemiluminescence method is reported using luminol-Cu(II) for the determination of vitamins A and C in pharmaceutical formulations. The reaction is based on the enhancement effect of analyte in the production of anion radicals of Rose Bengal (RB•−) which rapidly interact with dissolved oxygen and generate superoxide anions radicals (O2•−) and hydrogen peroxide (H2O2). Highly reactive hydroxyl radicals (•OH) were produced via dismutation of H2O2 by catalyst (Cu2+). The generated superoxide anions radicals and hydroxyl radicals thus oxidize luminol in alkaline medium to generate strong chemiluminescence. The limit of detection (3s of the blank, n=6) of vitamins A and C and RB was found to be 0.008, 0.005, and 0.05 μg mL−1, respectively. The sample throughput of 70 h−1 for vitamins A and C and 30 h−1 for RB was found. Calibration curve was linear in the range of 0.05–15, 0.01–20, and 0.1–50 μg mL−1 for vitamins A and C and RB, respectively, with relative standard deviations (RSDs; n=3) in the range 1.6–3.6%. The method was successfully applied to pharmaceutical formulations and the results obtained were in good agreement with the labeled values.http://dx.doi.org/10.1155/2014/109592 |
| spellingShingle | Muhammad Asgher Mohammad Yaqoob Abdul Nabi Ghulam Murtaza Abdul Rauf Siddiqi Amir Waseem Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination International Journal of Analytical Chemistry |
| title | Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination |
| title_full | Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination |
| title_fullStr | Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination |
| title_full_unstemmed | Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination |
| title_short | Flow Injection Photosensitized Chemiluminescence of Luminol with Cu(II)-Rose Bengal: Mechanistic Approach and Vitamin A and C Determination |
| title_sort | flow injection photosensitized chemiluminescence of luminol with cu ii rose bengal mechanistic approach and vitamin a and c determination |
| url | http://dx.doi.org/10.1155/2014/109592 |
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