Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?

Purpose. We aimed to investigate the possible effects of melatonin on gene expressions and activities of MnSOD and catalase under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in peripheral blood mononuclear cells (PBMCs). Materials and Methods. PBMCs were isolated from healthy...

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Main Authors: Solaleh Emamgholipour, Arash Hossein-Nezhad, Mohammad Ansari
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Biochemistry Research International
Online Access:http://dx.doi.org/10.1155/2016/5857940
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author Solaleh Emamgholipour
Arash Hossein-Nezhad
Mohammad Ansari
author_facet Solaleh Emamgholipour
Arash Hossein-Nezhad
Mohammad Ansari
author_sort Solaleh Emamgholipour
collection DOAJ
description Purpose. We aimed to investigate the possible effects of melatonin on gene expressions and activities of MnSOD and catalase under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in peripheral blood mononuclear cells (PBMCs). Materials and Methods. PBMCs were isolated from healthy subjects and treated as follows: (1) control (only with 0.1% DMSO for 12 h); (2) melatonin (1 mM) for 12 h; (3) H2O2 (250 μM) for 2 h; (4) H2O2 (250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM). The gene expression was evaluated by real-time PCR. MnSOD and catalase activities in PBMCs were determined by colorimetric assays. Results. Pretreatment of PBMCs with melatonin significantly augmented expression and activity of MnSOD which were diminished by H2O2. Melatonin treatment of PBMCs caused a significant upregulation of catalase by almost 2-fold in comparison with untreated cells. However, activity and expression of catalase increased by 1.5-fold in PBMCs under H2O2-induced oxidative stress compared with untreated cell. Moreover, pretreatment of PBMCs with melatonin resulted in a significant 1.8-fold increase in catalase expression compared to PBMCs treated only with H2O2. Conclusion. It seems that melatonin could prevent from undesirable impacts of H2O2-induced oxidative stress on MnSOD downregulation. Moreover, melatonin could promote inductive effect of H2O2 on catalase mRNA expression.
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spelling doaj-art-0a0d892c791b4707bffebfeb62b127f32025-02-03T01:22:37ZengWileyBiochemistry Research International2090-22472090-22552016-01-01201610.1155/2016/58579405857940Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?Solaleh Emamgholipour0Arash Hossein-Nezhad1Mohammad Ansari2Department of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, P.O. Box 14155-6447, Tehran, IranDepartment of Medicine, Section of Endocrinology, Nutrition, and Diabetes, Vitamin D, Skin and Bone Research Laboratory, Boston University Medical Center, 85 E. Newton Street Fuller Building, Boston, MA 02118, USADepartment of Clinical Biochemistry, School of Medicine, Tehran University of Medical Sciences, P.O. Box 14155-6447, Tehran, IranPurpose. We aimed to investigate the possible effects of melatonin on gene expressions and activities of MnSOD and catalase under conditions of oxidative stress induced by hydrogen peroxide (H2O2) in peripheral blood mononuclear cells (PBMCs). Materials and Methods. PBMCs were isolated from healthy subjects and treated as follows: (1) control (only with 0.1% DMSO for 12 h); (2) melatonin (1 mM) for 12 h; (3) H2O2 (250 μM) for 2 h; (4) H2O2 (250 μM) for 2 h following 10 h pretreatment with melatonin (1 mM). The gene expression was evaluated by real-time PCR. MnSOD and catalase activities in PBMCs were determined by colorimetric assays. Results. Pretreatment of PBMCs with melatonin significantly augmented expression and activity of MnSOD which were diminished by H2O2. Melatonin treatment of PBMCs caused a significant upregulation of catalase by almost 2-fold in comparison with untreated cells. However, activity and expression of catalase increased by 1.5-fold in PBMCs under H2O2-induced oxidative stress compared with untreated cell. Moreover, pretreatment of PBMCs with melatonin resulted in a significant 1.8-fold increase in catalase expression compared to PBMCs treated only with H2O2. Conclusion. It seems that melatonin could prevent from undesirable impacts of H2O2-induced oxidative stress on MnSOD downregulation. Moreover, melatonin could promote inductive effect of H2O2 on catalase mRNA expression.http://dx.doi.org/10.1155/2016/5857940
spellingShingle Solaleh Emamgholipour
Arash Hossein-Nezhad
Mohammad Ansari
Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
Biochemistry Research International
title Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
title_full Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
title_fullStr Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
title_full_unstemmed Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
title_short Can Melatonin Act as an Antioxidant in Hydrogen Peroxide-Induced Oxidative Stress Model in Human Peripheral Blood Mononuclear Cells?
title_sort can melatonin act as an antioxidant in hydrogen peroxide induced oxidative stress model in human peripheral blood mononuclear cells
url http://dx.doi.org/10.1155/2016/5857940
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AT arashhosseinnezhad canmelatoninactasanantioxidantinhydrogenperoxideinducedoxidativestressmodelinhumanperipheralbloodmononuclearcells
AT mohammadansari canmelatoninactasanantioxidantinhydrogenperoxideinducedoxidativestressmodelinhumanperipheralbloodmononuclearcells