PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION
Decidual and placental macrophage pools are renewed due to its transendothelial monocyte migration from peripheral blood. Tissue macrophages control placental development and provide fetomaternal immunological tolerance. Preeclamptic pregnancy is accompanied by increased monocyte migration to decidu...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
|
| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/90 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849241456349282304 |
|---|---|
| author | O. I. Stepanova G. R. Kozonov D. Z. Tsitskarava T. U. Kuzminykh D. A. Korenkov S. A. Selkov D. I. Sokolov |
| author_facet | O. I. Stepanova G. R. Kozonov D. Z. Tsitskarava T. U. Kuzminykh D. A. Korenkov S. A. Selkov D. I. Sokolov |
| author_sort | O. I. Stepanova |
| collection | DOAJ |
| description | Decidual and placental macrophage pools are renewed due to its transendothelial monocyte migration from peripheral blood. Tissue macrophages control placental development and provide fetomaternal immunological tolerance. Preeclamptic pregnancy is accompanied by increased monocyte migration to decidual tissue and local inflammatory events. Regulatory mechanisms of monocyte recruitment to placental and decidual tissues is still unclear. Therefore we investigated the influence soluble placental factors (SPFs) during the first- and third-trimester normal pregnancy, as compared to effects of these factors in preeclamptic pregnancy. We studied biological actions of SPF upon transendothelial migration of monocyte-like THP-1 cells and their phenotypic pattern. Transendothelial migration of THP-1 cells was more intensive with firsttrimester SPFs from normal pregnancy, when compared with third-trimester samples, and it was accompanied by decreased CD11a expression. SPFs from pre-eclamptic pregnancy caused an increase in transendothelial migration of THP-1 cells, as compared to SPFs from normal pregnancies, being accompanied by increased CD11b expression. The present study was supported by grants ГК № 02.740.11.0711, НШ-3594.2010.7, МД-150.2011.7 and a grant from St.-Petersburg Goverment for young scientists. |
| format | Article |
| id | doaj-art-0a09f668e9e14b4ea16ad4a33ce81689 |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-0a09f668e9e14b4ea16ad4a33ce816892025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0115213114010.15789/1563-0625-2013-2-131-14088PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATIONO. I. Stepanova0G. R. Kozonov1D. Z. Tsitskarava2T. U. Kuzminykh3D. A. Korenkov4S. A. Selkov5D. I. Sokolov6D. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgD. Ott Research Institute of Obstetrics and Gynecology, St. PetersburgDecidual and placental macrophage pools are renewed due to its transendothelial monocyte migration from peripheral blood. Tissue macrophages control placental development and provide fetomaternal immunological tolerance. Preeclamptic pregnancy is accompanied by increased monocyte migration to decidual tissue and local inflammatory events. Regulatory mechanisms of monocyte recruitment to placental and decidual tissues is still unclear. Therefore we investigated the influence soluble placental factors (SPFs) during the first- and third-trimester normal pregnancy, as compared to effects of these factors in preeclamptic pregnancy. We studied biological actions of SPF upon transendothelial migration of monocyte-like THP-1 cells and their phenotypic pattern. Transendothelial migration of THP-1 cells was more intensive with firsttrimester SPFs from normal pregnancy, when compared with third-trimester samples, and it was accompanied by decreased CD11a expression. SPFs from pre-eclamptic pregnancy caused an increase in transendothelial migration of THP-1 cells, as compared to SPFs from normal pregnancies, being accompanied by increased CD11b expression. The present study was supported by grants ГК № 02.740.11.0711, НШ-3594.2010.7, МД-150.2011.7 and a grant from St.-Petersburg Goverment for young scientists.https://www.mimmun.ru/mimmun/article/view/90placentapreeclampsiatransendothelial migrationthp-1 cells |
| spellingShingle | O. I. Stepanova G. R. Kozonov D. Z. Tsitskarava T. U. Kuzminykh D. A. Korenkov S. A. Selkov D. I. Sokolov PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION Медицинская иммунология placenta preeclampsia transendothelial migration thp-1 cells |
| title | PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION |
| title_full | PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION |
| title_fullStr | PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION |
| title_full_unstemmed | PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION |
| title_short | PLACENTAL SECRETORY FACTORS INFLUENCE TO THP-1 CELLS PHENOTYPE AND THP-1 CELLS TRANSENDOTHELIAL MIGRATION |
| title_sort | placental secretory factors influence to thp 1 cells phenotype and thp 1 cells transendothelial migration |
| topic | placenta preeclampsia transendothelial migration thp-1 cells |
| url | https://www.mimmun.ru/mimmun/article/view/90 |
| work_keys_str_mv | AT oistepanova placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT grkozonov placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT dztsitskarava placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT tukuzminykh placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT dakorenkov placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT saselkov placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration AT disokolov placentalsecretoryfactorsinfluencetothp1cellsphenotypeandthp1cellstransendothelialmigration |