Long-term and short-term effects of a unicellular symbiont on its beetle host

Abstract Gregarines are often host-specific, share a long coevolution with their host and can be viewed on a parasitism-mutualism spectrum. However, little is known about whether impacts on their hosts depend on the infection history over generations and thus transgenerational effects, or rather on...

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Main Authors: Alessa Barber, Etje Borsutzky, Caroline Müller
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-10427-x
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author Alessa Barber
Etje Borsutzky
Caroline Müller
author_facet Alessa Barber
Etje Borsutzky
Caroline Müller
author_sort Alessa Barber
collection DOAJ
description Abstract Gregarines are often host-specific, share a long coevolution with their host and can be viewed on a parasitism-mutualism spectrum. However, little is known about whether impacts on their hosts depend on the infection history over generations and thus transgenerational effects, or rather on the recent infection status. In the mustard leaf beetle, Phaedon cochleariae, more negative than positive effects of the gregarine species Gregarina cochlearium on its host were found in individuals infected over several generations compared to recently gregarine-freed individuals. Here, we tested the effects of the infection history of previous generations and the current infection status (not infected or infected with gregarines) on the performance, consumption, behavior and surface profiles of P. cochleariae, using a full factorial design. The current infection alone and in interaction with the infection history affected some performance parameters; developmental time was most prolonged in short-term infected individuals, while survival was highest in long-term infected individuals. Under a current gregarine infection adult female body mass was reduced, while reproduction was neither affected by the current infection nor infection history. With regard to consumption-related traits, the current infection status affected the growth rate of both sexes (in females also in interaction with infection history) and the consumption index of males, being lowest in currently infected individuals. Currently infected individuals also showed slightly higher activity with regard to some of the measured traits, while boldness-related traits were unaffected. No changes were found in surface profiles in dependence of the gregarine infection status. Overall, under the used conditions, the past experience with gregarines over generations seems to have only little impact on P. cochleariae, while the current infection status causes plastic responses, with negative consequences mostly on development. The study highlights that gregarines modulate the phenotypes of their host in different traits.
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spelling doaj-art-0a065ce1070943caa547bf2bed67ea9b2025-08-20T03:43:15ZengNature PortfolioScientific Reports2045-23222025-07-0115111610.1038/s41598-025-10427-xLong-term and short-term effects of a unicellular symbiont on its beetle hostAlessa Barber0Etje Borsutzky1Caroline Müller2Department of Chemical Ecology, Bielefeld UniversityDepartment of Chemical Ecology, Bielefeld UniversityDepartment of Chemical Ecology, Bielefeld UniversityAbstract Gregarines are often host-specific, share a long coevolution with their host and can be viewed on a parasitism-mutualism spectrum. However, little is known about whether impacts on their hosts depend on the infection history over generations and thus transgenerational effects, or rather on the recent infection status. In the mustard leaf beetle, Phaedon cochleariae, more negative than positive effects of the gregarine species Gregarina cochlearium on its host were found in individuals infected over several generations compared to recently gregarine-freed individuals. Here, we tested the effects of the infection history of previous generations and the current infection status (not infected or infected with gregarines) on the performance, consumption, behavior and surface profiles of P. cochleariae, using a full factorial design. The current infection alone and in interaction with the infection history affected some performance parameters; developmental time was most prolonged in short-term infected individuals, while survival was highest in long-term infected individuals. Under a current gregarine infection adult female body mass was reduced, while reproduction was neither affected by the current infection nor infection history. With regard to consumption-related traits, the current infection status affected the growth rate of both sexes (in females also in interaction with infection history) and the consumption index of males, being lowest in currently infected individuals. Currently infected individuals also showed slightly higher activity with regard to some of the measured traits, while boldness-related traits were unaffected. No changes were found in surface profiles in dependence of the gregarine infection status. Overall, under the used conditions, the past experience with gregarines over generations seems to have only little impact on P. cochleariae, while the current infection status causes plastic responses, with negative consequences mostly on development. The study highlights that gregarines modulate the phenotypes of their host in different traits.https://doi.org/10.1038/s41598-025-10427-xGregarinesCo-evolutionColeopteraHost-parasiteMutualistPerformance
spellingShingle Alessa Barber
Etje Borsutzky
Caroline Müller
Long-term and short-term effects of a unicellular symbiont on its beetle host
Scientific Reports
Gregarines
Co-evolution
Coleoptera
Host-parasite
Mutualist
Performance
title Long-term and short-term effects of a unicellular symbiont on its beetle host
title_full Long-term and short-term effects of a unicellular symbiont on its beetle host
title_fullStr Long-term and short-term effects of a unicellular symbiont on its beetle host
title_full_unstemmed Long-term and short-term effects of a unicellular symbiont on its beetle host
title_short Long-term and short-term effects of a unicellular symbiont on its beetle host
title_sort long term and short term effects of a unicellular symbiont on its beetle host
topic Gregarines
Co-evolution
Coleoptera
Host-parasite
Mutualist
Performance
url https://doi.org/10.1038/s41598-025-10427-x
work_keys_str_mv AT alessabarber longtermandshorttermeffectsofaunicellularsymbiontonitsbeetlehost
AT etjeborsutzky longtermandshorttermeffectsofaunicellularsymbiontonitsbeetlehost
AT carolinemuller longtermandshorttermeffectsofaunicellularsymbiontonitsbeetlehost