The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail

Consumers tend to favor natural dietary supplements to improve their health. However, vendors rarely cite scientific evidence to justify these claims. In the case of poppy oil, it is often mentioned as having a positive effect on Ca metabolism-related disorders, but no proof has been provided. There...

Full description

Saved in:
Bibliographic Details
Main Authors: Csaba Szabó, Xénia Ozsváth, Brigitta Csernus, Gabriella Gulyás, Márta Horváth, Levente Czeglédi, János Oláh, Nafiatur Rizqoh, Gabriele Achille, János Posta
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Animals
Subjects:
Online Access:https://www.mdpi.com/2076-2615/15/9/1348
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1850156010203774976
author Csaba Szabó
Xénia Ozsváth
Brigitta Csernus
Gabriella Gulyás
Márta Horváth
Levente Czeglédi
János Oláh
Nafiatur Rizqoh
Gabriele Achille
János Posta
author_facet Csaba Szabó
Xénia Ozsváth
Brigitta Csernus
Gabriella Gulyás
Márta Horváth
Levente Czeglédi
János Oláh
Nafiatur Rizqoh
Gabriele Achille
János Posta
author_sort Csaba Szabó
collection DOAJ
description Consumers tend to favor natural dietary supplements to improve their health. However, vendors rarely cite scientific evidence to justify these claims. In the case of poppy oil, it is often mentioned as having a positive effect on Ca metabolism-related disorders, but no proof has been provided. Therefore, the aim of our trial was to test the possible effect of poppy oil on calcium metabolism using Japanese quail layers as a model animal. A total of 120 four-week-old quail were divided into three dietary treatments (four cages per treatment, with ten birds in each cage): a control group (using sunflower oil as the energy source in the diet), 0.5%, and 1% poppy oil supplementation (replacing sunflower oil). Egg production, eggshell thickness, eggshell strength, and egg yolk color were investigated. Ca retention was determined using the acid-insoluble ash method. At the end of the experiment, two birds per cage were sacrificed, and uterus and jejunum samples were collected for gene expression analyses. Poppy oil supplementation improved egg production in terms of intensity, egg weight, and eggmass production. The thickness and eggshell strength decreased when 1% poppy oil was fed to the animals, while Ca retention improved. Poppy oil supplementation increased the expression of Ca transporter genes (<i>CALB1</i>, <i>SLC8A1</i>, and <i>SLC26A9</i>) in the uterus and <i>ITPR1</i> in the jejunum. Our results indicate a possible effect of poppy oil on Ca metabolism. Further studies are needed to identify the active compound and to understand the mode of action.
format Article
id doaj-art-0a04548c7fb6450494e2ec6a0e7bdeb8
institution OA Journals
issn 2076-2615
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Animals
spelling doaj-art-0a04548c7fb6450494e2ec6a0e7bdeb82025-08-20T02:24:43ZengMDPI AGAnimals2076-26152025-05-01159134810.3390/ani15091348The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese QuailCsaba Szabó0Xénia Ozsváth1Brigitta Csernus2Gabriella Gulyás3Márta Horváth4Levente Czeglédi5János Oláh6Nafiatur Rizqoh7Gabriele Achille8János Posta9Department of Animal Nutrition and Physiology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryDepartment of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryDepartment of Evolutionary Zoology and Human Biology, Faculty of Science and Technology, University of Debrecen, 4032 Debrecen, HungaryDepartment of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryDepartment of Animal Nutrition and Physiology, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryDepartment of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryKismacs Experimental Station of Animal Husbandry, Institute for Agricultural Research and Educational Farm, University of Debrecen, 4032 Debrecen, HungaryDepartment of Animal Science and Biotechnology, Kyungpook National University, Sangju 37224, Republic of KoreaLaboratory of Protistology and Biology Education, University of Macerata, 62100 Macerata, ItalyDepartment of Animal Husbandry, Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, 4032 Debrecen, HungaryConsumers tend to favor natural dietary supplements to improve their health. However, vendors rarely cite scientific evidence to justify these claims. In the case of poppy oil, it is often mentioned as having a positive effect on Ca metabolism-related disorders, but no proof has been provided. Therefore, the aim of our trial was to test the possible effect of poppy oil on calcium metabolism using Japanese quail layers as a model animal. A total of 120 four-week-old quail were divided into three dietary treatments (four cages per treatment, with ten birds in each cage): a control group (using sunflower oil as the energy source in the diet), 0.5%, and 1% poppy oil supplementation (replacing sunflower oil). Egg production, eggshell thickness, eggshell strength, and egg yolk color were investigated. Ca retention was determined using the acid-insoluble ash method. At the end of the experiment, two birds per cage were sacrificed, and uterus and jejunum samples were collected for gene expression analyses. Poppy oil supplementation improved egg production in terms of intensity, egg weight, and eggmass production. The thickness and eggshell strength decreased when 1% poppy oil was fed to the animals, while Ca retention improved. Poppy oil supplementation increased the expression of Ca transporter genes (<i>CALB1</i>, <i>SLC8A1</i>, and <i>SLC26A9</i>) in the uterus and <i>ITPR1</i> in the jejunum. Our results indicate a possible effect of poppy oil on Ca metabolism. Further studies are needed to identify the active compound and to understand the mode of action.https://www.mdpi.com/2076-2615/15/9/1348poppy oilegg productionegg qualitycalciumJapanese quail
spellingShingle Csaba Szabó
Xénia Ozsváth
Brigitta Csernus
Gabriella Gulyás
Márta Horváth
Levente Czeglédi
János Oláh
Nafiatur Rizqoh
Gabriele Achille
János Posta
The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
Animals
poppy oil
egg production
egg quality
calcium
Japanese quail
title The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
title_full The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
title_fullStr The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
title_full_unstemmed The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
title_short The Effect of Poppy Oil on Egg Production and Calcium Metabolism in Japanese Quail
title_sort effect of poppy oil on egg production and calcium metabolism in japanese quail
topic poppy oil
egg production
egg quality
calcium
Japanese quail
url https://www.mdpi.com/2076-2615/15/9/1348
work_keys_str_mv AT csabaszabo theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT xeniaozsvath theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT brigittacsernus theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT gabriellagulyas theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT martahorvath theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT leventeczegledi theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT janosolah theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT nafiaturrizqoh theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT gabrieleachille theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT janosposta theeffectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT csabaszabo effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT xeniaozsvath effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT brigittacsernus effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT gabriellagulyas effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT martahorvath effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT leventeczegledi effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT janosolah effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT nafiaturrizqoh effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT gabrieleachille effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail
AT janosposta effectofpoppyoiloneggproductionandcalciummetabolisminjapanesequail