Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells
The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/425421 |
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| author | Andressa Vilas Boas Nogueira Marjan Nokhbehsaim Sigrun Eick Christoph Bourauel Andreas Jäger Søren Jepsen Carlos Rossa James Deschner Joni Augusto Cirelli |
| author_facet | Andressa Vilas Boas Nogueira Marjan Nokhbehsaim Sigrun Eick Christoph Bourauel Andreas Jäger Søren Jepsen Carlos Rossa James Deschner Joni Augusto Cirelli |
| author_sort | Andressa Vilas Boas Nogueira |
| collection | DOAJ |
| description | The present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days. Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA. Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively. F. nucleatum increased the production of COX2 and PGE2, which was further increased by CTS. F. nucleatum-induced increase of PGE2 synthesis was significantly (P<0.05) increased when CTSH was applied at 1 and 3 days. In addition, CTSH inhibited the F. nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio. OPG and RANKL mRNA results correlated with the protein results. In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells. Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis. |
| format | Article |
| id | doaj-art-09fcb8288b2e4a1b91acf503534bf2e7 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-09fcb8288b2e4a1b91acf503534bf2e72025-08-20T03:55:27ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/425421425421Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL CellsAndressa Vilas Boas Nogueira0Marjan Nokhbehsaim1Sigrun Eick2Christoph Bourauel3Andreas Jäger4Søren Jepsen5Carlos Rossa6James Deschner7Joni Augusto Cirelli8Department of Diagnosis and Surgery, School of Dentistry at Araraquara, Univ Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilSection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyDepartment of Periodontology, Oral Microbiology, University of Bern, 3010 Bern, SwitzerlandClinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyClinical Research Unit 208, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Univ Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilSection of Experimental Dento-Maxillo-Facial Medicine, Center of Dento-Maxillo-Facial Medicine, University of Bonn, 53111 Bonn, GermanyDepartment of Diagnosis and Surgery, School of Dentistry at Araraquara, Univ Estadual Paulista (UNESP), 14801-903 Araraquara, SP, BrazilThe present study aimed to evaluate in vitro whether biomechanical loading modulates proinflammatory and bone remodeling mediators production by periodontal ligament (PDL) cells in the presence of bacterial challenge. Cells were seeded on BioFlex culture plates and exposed to Fusobacterium nucleatum ATCC 25586 and/or cyclic tensile strain (CTS) of low (CTSL) and high (CTSH) magnitudes for 1 and 3 days. Synthesis of cyclooxygenase-2 (COX2) and prostaglandin E2 (PGE2) was evaluated by ELISA. Gene expression and protein secretion of osteoprotegerin (OPG) and receptor activator of nuclear factor kappa-B ligand (RANKL) were evaluated by quantitative RT-PCR and ELISA, respectively. F. nucleatum increased the production of COX2 and PGE2, which was further increased by CTS. F. nucleatum-induced increase of PGE2 synthesis was significantly (P<0.05) increased when CTSH was applied at 1 and 3 days. In addition, CTSH inhibited the F. nucleatum-induced upregulation of OPG at 1 and 3 days, thereby increasing the RANKL/OPG ratio. OPG and RANKL mRNA results correlated with the protein results. In summary, our findings provide original evidence that CTS can enhance bacterial-induced syntheses of molecules associated with inflammation and bone resorption by PDL cells. Therefore, biomechanical, such as orthodontic or occlusal, loading may enhance the bacterial-induced inflammation and destruction in periodontitis.http://dx.doi.org/10.1155/2014/425421 |
| spellingShingle | Andressa Vilas Boas Nogueira Marjan Nokhbehsaim Sigrun Eick Christoph Bourauel Andreas Jäger Søren Jepsen Carlos Rossa James Deschner Joni Augusto Cirelli Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells Mediators of Inflammation |
| title | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells |
| title_full | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells |
| title_fullStr | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells |
| title_full_unstemmed | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells |
| title_short | Biomechanical Loading Modulates Proinflammatory and Bone Resorptive Mediators in Bacterial-Stimulated PDL Cells |
| title_sort | biomechanical loading modulates proinflammatory and bone resorptive mediators in bacterial stimulated pdl cells |
| url | http://dx.doi.org/10.1155/2014/425421 |
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