Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability

The milk fat globule membrane (MFGM) has been shown to improve probiotic survival in the gastrointestinal tract. However, emulsions stabilized through electrostatic interactions between MFGM and polysaccharides for probiotic encapsulation remain unexplored. This study established optimal conditions...

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Main Authors: Yu Ji, Yifan Wu, Yan Wang, Shuangshuang He, Yishan Jiang, Xin Li, Liupeng Wang, Qian Xu, Lili Zhang, Hao Wang
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Food Chemistry: X
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Online Access:http://www.sciencedirect.com/science/article/pii/S2590157525002561
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author Yu Ji
Yifan Wu
Yan Wang
Shuangshuang He
Yishan Jiang
Xin Li
Liupeng Wang
Qian Xu
Lili Zhang
Hao Wang
author_facet Yu Ji
Yifan Wu
Yan Wang
Shuangshuang He
Yishan Jiang
Xin Li
Liupeng Wang
Qian Xu
Lili Zhang
Hao Wang
author_sort Yu Ji
collection DOAJ
description The milk fat globule membrane (MFGM) has been shown to improve probiotic survival in the gastrointestinal tract. However, emulsions stabilized through electrostatic interactions between MFGM and polysaccharides for probiotic encapsulation remain unexplored. This study established optimal conditions for creating MFGM-Pectin (CP) emulsions. At pH 4.0, a MFGM/CP ratio of 1 produced the smallest particle size and the highest zeta potential, ideal for emulsion stabilization. We evaluated the effects of varying complex concentrations and oil phase ratios on emulsion properties. The results indicate that CP significantly affects the apparent viscosity, oxidative stability, centrifugal stability, storage stability, and antioxidant activity of MFGM-CP emulsions. Furthermore, the encapsulation of LGG within the emulsion improved its survival and storage stability in simulated gastrointestinal fluids. These findings suggest that MFGM is a promising material for probiotic encapsulation and provide a foundation for developing MFGM-based products containing probiotics or other active ingredients.
format Article
id doaj-art-09fa4705128f4a92a9a276b6af577cc3
institution OA Journals
issn 2590-1575
language English
publishDate 2025-04-01
publisher Elsevier
record_format Article
series Food Chemistry: X
spelling doaj-art-09fa4705128f4a92a9a276b6af577cc32025-08-20T02:31:26ZengElsevierFood Chemistry: X2590-15752025-04-012710240910.1016/j.fochx.2025.102409Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stabilityYu Ji0Yifan Wu1Yan Wang2Shuangshuang He3Yishan Jiang4Xin Li5Liupeng Wang6Qian Xu7Lili Zhang8Hao Wang9Key Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, ChinaCollege of Food Science and Engineering, Tarim University, Alaer 843300, ChinaKey Laboratory of Dairy Science, College of Food Science, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.College of Life Science, Northeast Agricultural University, Harbin 150030, China; Corresponding authors.The milk fat globule membrane (MFGM) has been shown to improve probiotic survival in the gastrointestinal tract. However, emulsions stabilized through electrostatic interactions between MFGM and polysaccharides for probiotic encapsulation remain unexplored. This study established optimal conditions for creating MFGM-Pectin (CP) emulsions. At pH 4.0, a MFGM/CP ratio of 1 produced the smallest particle size and the highest zeta potential, ideal for emulsion stabilization. We evaluated the effects of varying complex concentrations and oil phase ratios on emulsion properties. The results indicate that CP significantly affects the apparent viscosity, oxidative stability, centrifugal stability, storage stability, and antioxidant activity of MFGM-CP emulsions. Furthermore, the encapsulation of LGG within the emulsion improved its survival and storage stability in simulated gastrointestinal fluids. These findings suggest that MFGM is a promising material for probiotic encapsulation and provide a foundation for developing MFGM-based products containing probiotics or other active ingredients.http://www.sciencedirect.com/science/article/pii/S2590157525002561Milk fat globule membranePectinPickering emulsionProbioticsEncapsulation
spellingShingle Yu Ji
Yifan Wu
Yan Wang
Shuangshuang He
Yishan Jiang
Xin Li
Liupeng Wang
Qian Xu
Lili Zhang
Hao Wang
Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
Food Chemistry: X
Milk fat globule membrane
Pectin
Pickering emulsion
Probiotics
Encapsulation
title Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
title_full Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
title_fullStr Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
title_full_unstemmed Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
title_short Pickering emulsion gel stabilized by milk fat globule membrane/pectin enhanced probiotic stability
title_sort pickering emulsion gel stabilized by milk fat globule membrane pectin enhanced probiotic stability
topic Milk fat globule membrane
Pectin
Pickering emulsion
Probiotics
Encapsulation
url http://www.sciencedirect.com/science/article/pii/S2590157525002561
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