Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries.
<h4>Context</h4>Estrogen receptor α (ERα) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear.<h4>Aims</h4>The study aims to elucidate the molecular mechanisms of ER...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2024-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0313867 |
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| author | Qicai Hu Yiqian Gui Congcong Cao Jun Xie Huiru Tang |
| author_facet | Qicai Hu Yiqian Gui Congcong Cao Jun Xie Huiru Tang |
| author_sort | Qicai Hu |
| collection | DOAJ |
| description | <h4>Context</h4>Estrogen receptor α (ERα) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear.<h4>Aims</h4>The study aims to elucidate the molecular mechanisms of ERα-regulated transcriptional dynamics in ovarian cells using ERα knockout (αERKO) mice created via CRISPR/Cas9.<h4>Methods</h4>Single-cell RNA sequencing (scRNA-seq) was used to compare transcriptomes from individual ovarian cells in both wild type and αERKO mice. Bioinformatics analyses identified distinct cell populations and their transcriptional profiles post ERα deletion.<h4>Key results</h4>Distinct oocyte and granulosa cell populations were identified, with ERα deletion disrupting the regulation of genes linked to ovarian infertility, the ovulation cycle, and steroidogenesis. Greb1 expression in granulosa cells was found to be ERα-dependent.<h4>Conclusions</h4>ERα deletion significantly alters the transcriptional landscape of ovarian cells, affecting genes and pathways central to ovarian function and the ovulation process.<h4>Implications</h4>The findings provide an in-depth, single-cell view of ERα's role in the reproductive system, offering insights that may lead to novel treatments for ovarian disorders. |
| format | Article |
| id | doaj-art-09f4cb0727af4e159214112a8c6c8d27 |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-09f4cb0727af4e159214112a8c6c8d272025-08-20T02:22:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032024-01-011911e031386710.1371/journal.pone.0313867Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries.Qicai HuYiqian GuiCongcong CaoJun XieHuiru Tang<h4>Context</h4>Estrogen receptor α (ERα) is a key regulator of reproductive function, particularly in ovarian development and function, yet the specifics of its role at the molecular level remain unclear.<h4>Aims</h4>The study aims to elucidate the molecular mechanisms of ERα-regulated transcriptional dynamics in ovarian cells using ERα knockout (αERKO) mice created via CRISPR/Cas9.<h4>Methods</h4>Single-cell RNA sequencing (scRNA-seq) was used to compare transcriptomes from individual ovarian cells in both wild type and αERKO mice. Bioinformatics analyses identified distinct cell populations and their transcriptional profiles post ERα deletion.<h4>Key results</h4>Distinct oocyte and granulosa cell populations were identified, with ERα deletion disrupting the regulation of genes linked to ovarian infertility, the ovulation cycle, and steroidogenesis. Greb1 expression in granulosa cells was found to be ERα-dependent.<h4>Conclusions</h4>ERα deletion significantly alters the transcriptional landscape of ovarian cells, affecting genes and pathways central to ovarian function and the ovulation process.<h4>Implications</h4>The findings provide an in-depth, single-cell view of ERα's role in the reproductive system, offering insights that may lead to novel treatments for ovarian disorders.https://doi.org/10.1371/journal.pone.0313867 |
| spellingShingle | Qicai Hu Yiqian Gui Congcong Cao Jun Xie Huiru Tang Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. PLoS ONE |
| title | Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. |
| title_full | Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. |
| title_fullStr | Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. |
| title_full_unstemmed | Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. |
| title_short | Single-cell sequencing reveals transcriptional dynamics regulated by ERα in mouse ovaries. |
| title_sort | single cell sequencing reveals transcriptional dynamics regulated by erα in mouse ovaries |
| url | https://doi.org/10.1371/journal.pone.0313867 |
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