Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil.
The microbial and molecular characterization of the ectorhizosphere is an important step towards developing a more complete understanding of how the cultivation of biofuel crops can be undertaken in nutrient poor environments. The ectorhizosphere of Setaria is of particular interest because the plan...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2021-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259937&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849708053375483904 |
|---|---|
| author | Matthew J Peterson Pubudu P Handakumbura Allison M Thompson Zachary R Russell Young-Mo Kim Sarah J Fansler Montana L Smith Jason G Toyoda Rosey K Chu Bryan A Stanfill Steven C Fransen Vanessa L Bailey Christer Jansson Kim K Hixson Stephen J Callister |
| author_facet | Matthew J Peterson Pubudu P Handakumbura Allison M Thompson Zachary R Russell Young-Mo Kim Sarah J Fansler Montana L Smith Jason G Toyoda Rosey K Chu Bryan A Stanfill Steven C Fransen Vanessa L Bailey Christer Jansson Kim K Hixson Stephen J Callister |
| author_sort | Matthew J Peterson |
| collection | DOAJ |
| description | The microbial and molecular characterization of the ectorhizosphere is an important step towards developing a more complete understanding of how the cultivation of biofuel crops can be undertaken in nutrient poor environments. The ectorhizosphere of Setaria is of particular interest because the plant component of this plant-microbe system is an important agricultural grain crop and a model for biofuel grasses. Importantly, Setaria lends itself to high throughput molecular studies. As such, we have identified important intra- and interspecific microbial and molecular differences in the ectorhizospheres of three geographically distant Setaria italica accessions and their wild ancestor S. viridis. All were grown in a nutrient-poor soil with and without nutrient addition. To assess the contrasting impact of nutrient deficiency observed for two S. italica accessions, we quantitatively evaluated differences in soil organic matter, microbial community, and metabolite profiles. Together, these measurements suggest that rhizosphere priming differs with Setaria accession, which comes from alterations in microbial community abundances, specifically Actinobacteria and Proteobacteria populations. When globally comparing the metabolomic response of Setaria to nutrient addition, plants produced distinctly different metabolic profiles in the leaves and roots. With nutrient addition, increases of nitrogen containing metabolites were significantly higher in plant leaves and roots along with significant increases in tyrosine derived alkaloids, serotonin, and synephrine. Glycerol was also found to be significantly increased in the leaves as well as the ectorhizosphere. These differences provide insight into how C4 grasses adapt to changing nutrient availability in soils or with contrasting fertilization schemas. Gained knowledge could then be utilized in plant enhancement and bioengineering efforts to produce plants with superior traits when grown in nutrient poor soils. |
| format | Article |
| id | doaj-art-09f486ab47a34b8fb53fc0e95b1770fb |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-09f486ab47a34b8fb53fc0e95b1770fb2025-08-20T03:15:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011612e025993710.1371/journal.pone.0259937Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil.Matthew J PetersonPubudu P HandakumburaAllison M ThompsonZachary R RussellYoung-Mo KimSarah J FanslerMontana L SmithJason G ToyodaRosey K ChuBryan A StanfillSteven C FransenVanessa L BaileyChrister JanssonKim K HixsonStephen J CallisterThe microbial and molecular characterization of the ectorhizosphere is an important step towards developing a more complete understanding of how the cultivation of biofuel crops can be undertaken in nutrient poor environments. The ectorhizosphere of Setaria is of particular interest because the plant component of this plant-microbe system is an important agricultural grain crop and a model for biofuel grasses. Importantly, Setaria lends itself to high throughput molecular studies. As such, we have identified important intra- and interspecific microbial and molecular differences in the ectorhizospheres of three geographically distant Setaria italica accessions and their wild ancestor S. viridis. All were grown in a nutrient-poor soil with and without nutrient addition. To assess the contrasting impact of nutrient deficiency observed for two S. italica accessions, we quantitatively evaluated differences in soil organic matter, microbial community, and metabolite profiles. Together, these measurements suggest that rhizosphere priming differs with Setaria accession, which comes from alterations in microbial community abundances, specifically Actinobacteria and Proteobacteria populations. When globally comparing the metabolomic response of Setaria to nutrient addition, plants produced distinctly different metabolic profiles in the leaves and roots. With nutrient addition, increases of nitrogen containing metabolites were significantly higher in plant leaves and roots along with significant increases in tyrosine derived alkaloids, serotonin, and synephrine. Glycerol was also found to be significantly increased in the leaves as well as the ectorhizosphere. These differences provide insight into how C4 grasses adapt to changing nutrient availability in soils or with contrasting fertilization schemas. Gained knowledge could then be utilized in plant enhancement and bioengineering efforts to produce plants with superior traits when grown in nutrient poor soils.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259937&type=printable |
| spellingShingle | Matthew J Peterson Pubudu P Handakumbura Allison M Thompson Zachary R Russell Young-Mo Kim Sarah J Fansler Montana L Smith Jason G Toyoda Rosey K Chu Bryan A Stanfill Steven C Fransen Vanessa L Bailey Christer Jansson Kim K Hixson Stephen J Callister Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. PLoS ONE |
| title | Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. |
| title_full | Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. |
| title_fullStr | Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. |
| title_full_unstemmed | Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. |
| title_short | Deciphering the microbial and molecular responses of geographically diverse Setaria accessions grown in a nutrient-poor soil. |
| title_sort | deciphering the microbial and molecular responses of geographically diverse setaria accessions grown in a nutrient poor soil |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0259937&type=printable |
| work_keys_str_mv | AT matthewjpeterson decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT pubuduphandakumbura decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT allisonmthompson decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT zacharyrrussell decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT youngmokim decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT sarahjfansler decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT montanalsmith decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT jasongtoyoda decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT roseykchu decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT bryanastanfill decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT stevencfransen decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT vanessalbailey decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT christerjansson decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT kimkhixson decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil AT stephenjcallister decipheringthemicrobialandmolecularresponsesofgeographicallydiversesetariaaccessionsgrowninanutrientpoorsoil |