Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms
Although thiazolidinediones (TZDs) were found to be ligands for peroxisome proliferators-activated receptorγ (PPARγ), the mechanism by which TZDs exert their anticancer effect remains unclear. Furthermore, the effect of TZDs on metastatic and angiogenesis potential of cancer cells is unknown. Our re...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2008-01-01
|
| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2008/649808 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849305275790524416 |
|---|---|
| author | Qing He Ruiping Pang Xin Song Jie Chen Huixin Chen Baili Chen Pinjin Hu Minhu Chen |
| author_facet | Qing He Ruiping Pang Xin Song Jie Chen Huixin Chen Baili Chen Pinjin Hu Minhu Chen |
| author_sort | Qing He |
| collection | DOAJ |
| description | Although thiazolidinediones (TZDs) were found to be ligands for peroxisome proliferators-activated receptorγ (PPARγ), the mechanism by which TZDs exert their anticancer effect remains unclear. Furthermore, the effect of TZDs on metastatic and angiogenesis potential of cancer cells is unknown. Our results in this paper show that rosiglitazone inhibited SGC-7901 gastric cancer cells growth, caused G1 cell cycle arrest and induced apoptosis in a dose-dependent manner. The effects of rosiglitazone on SGC-7901 cancer cells were completely reversed by treatment with PPARγ antagonist GW9662. Rosiglitazone inhibited SGC-7901 cell migration, invasiveness, and the expression of MMP-2 in dose-dependent manner via PPARγ-independent manner. Rosiglitazone reduced the VEGF induced angiogenesis of HUVEC in dose-dependent manner through PPARγ-dependent pathway. Moreover, rosiglitazone did not affect the expression of VEGF by SGC-7901 cells. Our results demonstrated that by PPARγ ligand, rosiglitazone inhibited growth and invasiveness of SGC-7901 gastric cancer cells and angiogenesis in vitro via PPARγ-dependent or -independent pathway. |
| format | Article |
| id | doaj-art-09d33b7d4f464981a6ecd93c8f1b4643 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2008-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-09d33b7d4f464981a6ecd93c8f1b46432025-08-20T03:55:29ZengWileyPPAR Research1687-47571687-47652008-01-01200810.1155/2008/649808649808Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent MechanismsQing He0Ruiping Pang1Xin Song2Jie Chen3Huixin Chen4Baili Chen5Pinjin Hu6Minhu Chen7Department of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Physiology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaDepartment of Gastroenterology, The First Affiliated Hospital, Sun Yat-Sen University, Guangzhou 510080, ChinaAlthough thiazolidinediones (TZDs) were found to be ligands for peroxisome proliferators-activated receptorγ (PPARγ), the mechanism by which TZDs exert their anticancer effect remains unclear. Furthermore, the effect of TZDs on metastatic and angiogenesis potential of cancer cells is unknown. Our results in this paper show that rosiglitazone inhibited SGC-7901 gastric cancer cells growth, caused G1 cell cycle arrest and induced apoptosis in a dose-dependent manner. The effects of rosiglitazone on SGC-7901 cancer cells were completely reversed by treatment with PPARγ antagonist GW9662. Rosiglitazone inhibited SGC-7901 cell migration, invasiveness, and the expression of MMP-2 in dose-dependent manner via PPARγ-independent manner. Rosiglitazone reduced the VEGF induced angiogenesis of HUVEC in dose-dependent manner through PPARγ-dependent pathway. Moreover, rosiglitazone did not affect the expression of VEGF by SGC-7901 cells. Our results demonstrated that by PPARγ ligand, rosiglitazone inhibited growth and invasiveness of SGC-7901 gastric cancer cells and angiogenesis in vitro via PPARγ-dependent or -independent pathway.http://dx.doi.org/10.1155/2008/649808 |
| spellingShingle | Qing He Ruiping Pang Xin Song Jie Chen Huixin Chen Baili Chen Pinjin Hu Minhu Chen Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms PPAR Research |
| title | Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms |
| title_full | Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms |
| title_fullStr | Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms |
| title_full_unstemmed | Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms |
| title_short | Rosiglitazone Suppresses the Growth and Invasiveness of SGC-7901 Gastric Cancer Cells and Angiogenesis In Vitro via PPARγ Dependent and Independent Mechanisms |
| title_sort | rosiglitazone suppresses the growth and invasiveness of sgc 7901 gastric cancer cells and angiogenesis in vitro via pparγ dependent and independent mechanisms |
| url | http://dx.doi.org/10.1155/2008/649808 |
| work_keys_str_mv | AT qinghe rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT ruipingpang rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT xinsong rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT jiechen rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT huixinchen rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT bailichen rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT pinjinhu rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms AT minhuchen rosiglitazonesuppressesthegrowthandinvasivenessofsgc7901gastriccancercellsandangiogenesisinvitroviappargdependentandindependentmechanisms |