Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent
A novel metal-organic framework (MOF) material, UiO-66-NH2, was synthesized using a solvothermal method. Subsequently, a high performance liquid chromatography (HPLC) method with solid phase extraction using UiO-66-NH2 was established for the simultaneous detection of five fluoroquinolone (FQ) resid...
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China Food Publishing Company
2025-02-01
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| author | NIU Canjie, YE Sudan, ZHU Qiancong, HU Yuxia |
| author_facet | NIU Canjie, YE Sudan, ZHU Qiancong, HU Yuxia |
| author_sort | NIU Canjie, YE Sudan, ZHU Qiancong, HU Yuxia |
| collection | DOAJ |
| description | A novel metal-organic framework (MOF) material, UiO-66-NH2, was synthesized using a solvothermal method. Subsequently, a high performance liquid chromatography (HPLC) method with solid phase extraction using UiO-66-NH2 was established for the simultaneous detection of five fluoroquinolone (FQ) residues in aquatic products. The sample was extracted with acetonitrile in 1.0% formic acid, degreased with n-hexane, and concentrated by rotary evaporation. The extract was then redissolved in aqueous ammonia solution at pH 8.0. The FQs were enriched and purified with a solid phase extraction (SPE) column filled with 30 mg of UiO-66-NH2 using 6 mL of 20% acetic acid-methanol solution as the eluent. The collected eluate was blown to dryness under nitrogen, redissolved and detected by HPLC with a fluorescence detector. Under the established experimental conditions, good linear relationships were achieved for the five FQs in the concentration range of 0.005–0.50 mg/L, with determination coefficients (R2) of 0.999 9–1.0. The recovery rates ranged from 81.4% to 104.8% with relative standard deviation (RSD) of 1.29%–4.93%, and the limits of detection (LOD) were in the range of 0.21–2.05 μg/kg. The prepared SPE column could be reused up to five times, which was environmentally friendly and cost-effective. The established method had good accuracy, precision, reproducibility, and selectivity, and was suitable for the simultaneous detection of various trace FQ residues in aquatic products. |
| format | Article |
| id | doaj-art-09c99d68400e4dc2a10cee75264f6a36 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-02-01 |
| publisher | China Food Publishing Company |
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| series | Shipin Kexue |
| spelling | doaj-art-09c99d68400e4dc2a10cee75264f6a362025-02-05T09:08:33ZengChina Food Publishing CompanyShipin Kexue1002-66302025-02-0146322222910.7506/spkx1002-6630-20240718-183Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as SorbentNIU Canjie, YE Sudan, ZHU Qiancong, HU Yuxia0(Zhejiang Institute of Economic and Trade, Hangzhou 310012, China)A novel metal-organic framework (MOF) material, UiO-66-NH2, was synthesized using a solvothermal method. Subsequently, a high performance liquid chromatography (HPLC) method with solid phase extraction using UiO-66-NH2 was established for the simultaneous detection of five fluoroquinolone (FQ) residues in aquatic products. The sample was extracted with acetonitrile in 1.0% formic acid, degreased with n-hexane, and concentrated by rotary evaporation. The extract was then redissolved in aqueous ammonia solution at pH 8.0. The FQs were enriched and purified with a solid phase extraction (SPE) column filled with 30 mg of UiO-66-NH2 using 6 mL of 20% acetic acid-methanol solution as the eluent. The collected eluate was blown to dryness under nitrogen, redissolved and detected by HPLC with a fluorescence detector. Under the established experimental conditions, good linear relationships were achieved for the five FQs in the concentration range of 0.005–0.50 mg/L, with determination coefficients (R2) of 0.999 9–1.0. The recovery rates ranged from 81.4% to 104.8% with relative standard deviation (RSD) of 1.29%–4.93%, and the limits of detection (LOD) were in the range of 0.21–2.05 μg/kg. The prepared SPE column could be reused up to five times, which was environmentally friendly and cost-effective. The established method had good accuracy, precision, reproducibility, and selectivity, and was suitable for the simultaneous detection of various trace FQ residues in aquatic products.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-025.pdfmetal-organic framework; solid phase extraction; high performance liquid chromatography; aquatic products; fluoroquinolones |
| spellingShingle | NIU Canjie, YE Sudan, ZHU Qiancong, HU Yuxia Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent Shipin Kexue metal-organic framework; solid phase extraction; high performance liquid chromatography; aquatic products; fluoroquinolones |
| title | Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent |
| title_full | Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent |
| title_fullStr | Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent |
| title_full_unstemmed | Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent |
| title_short | Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent |
| title_sort | determination of five fluoroquinolones in aquatic products by high performance liquid chromatography with solid phase extraction using metal organic framework as sorbent |
| topic | metal-organic framework; solid phase extraction; high performance liquid chromatography; aquatic products; fluoroquinolones |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-3-025.pdf |
| work_keys_str_mv | AT niucanjieyesudanzhuqianconghuyuxia determinationoffivefluoroquinolonesinaquaticproductsbyhighperformanceliquidchromatographywithsolidphaseextractionusingmetalorganicframeworkassorbent |