Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology
This study aimed to optimize the ultrasound-assisted extraction (UAE) process using food-grade ethanol to recover glucosinolates from upcycled cauliflower through response surface methodology. The optimized extraction process was compared with traditional extraction using maceration with solvents su...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/11/2326 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849331200252968960 |
|---|---|
| author | Hana Derbew Gedif Tess Astatkie Joanna Tkaczewska H. P. Vasantha Rupasinghe |
| author_facet | Hana Derbew Gedif Tess Astatkie Joanna Tkaczewska H. P. Vasantha Rupasinghe |
| author_sort | Hana Derbew Gedif |
| collection | DOAJ |
| description | This study aimed to optimize the ultrasound-assisted extraction (UAE) process using food-grade ethanol to recover glucosinolates from upcycled cauliflower through response surface methodology. The optimized extraction process was compared with traditional extraction using maceration with solvents such as methanol and acetone. The optimum UAE conditions identified for extracting glucosinolates from upcycled cauliflower were: 42% ethanol as solvent at 43 °C for 30 min. The total glucosinolate content recovered was 7400 μg sinigrin equivalence (SE)/g dry weight (DW) of biomass. The ultra-pressure liquid chromatography–electrospray ionization-mass spectrometry (UPLC-ESI-MS) analysis confirmed that the optimized UAE yielded the highest levels of glucoraphanin (1.31 ± 0.12 μg/g DW of biomass) and sulforaphane (28.2 ± 3.34 μg/g DW of biomass). The extracts possess greater antioxidant activity as determined by ferric reducing antioxidant power and DPPH radical scavenging activity. The optimized UAE process significantly enhanced the extraction of valuable phytochemical molecules from the upcycled cauliflower. Further studies should focus on evaluating their therapeutic and preventive potential for applications in nutrition and health. |
| format | Article |
| id | doaj-art-09c6e69bc50a4f20a2712feb503fc46d |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-09c6e69bc50a4f20a2712feb503fc46d2025-08-20T03:46:42ZengMDPI AGMolecules1420-30492025-05-013011232610.3390/molecules30112326Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface MethodologyHana Derbew Gedif0Tess Astatkie1Joanna Tkaczewska2H. P. Vasantha Rupasinghe3Department of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, CanadaFaculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, CanadaDepartment of Animal Product Technology, Faculty of Food Technology, University of Agriculture, Balicka 122, 30-149 Kraków, PolandDepartment of Plant, Food, and Environmental Sciences, Faculty of Agriculture, Dalhousie University, Truro, NS B2N 5E3, CanadaThis study aimed to optimize the ultrasound-assisted extraction (UAE) process using food-grade ethanol to recover glucosinolates from upcycled cauliflower through response surface methodology. The optimized extraction process was compared with traditional extraction using maceration with solvents such as methanol and acetone. The optimum UAE conditions identified for extracting glucosinolates from upcycled cauliflower were: 42% ethanol as solvent at 43 °C for 30 min. The total glucosinolate content recovered was 7400 μg sinigrin equivalence (SE)/g dry weight (DW) of biomass. The ultra-pressure liquid chromatography–electrospray ionization-mass spectrometry (UPLC-ESI-MS) analysis confirmed that the optimized UAE yielded the highest levels of glucoraphanin (1.31 ± 0.12 μg/g DW of biomass) and sulforaphane (28.2 ± 3.34 μg/g DW of biomass). The extracts possess greater antioxidant activity as determined by ferric reducing antioxidant power and DPPH radical scavenging activity. The optimized UAE process significantly enhanced the extraction of valuable phytochemical molecules from the upcycled cauliflower. Further studies should focus on evaluating their therapeutic and preventive potential for applications in nutrition and health.https://www.mdpi.com/1420-3049/30/11/2326<i>Brassica oleracea</i>sulforaphanegreen extractionfood wasteantioxidantsUPLC-ESI-MS |
| spellingShingle | Hana Derbew Gedif Tess Astatkie Joanna Tkaczewska H. P. Vasantha Rupasinghe Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology Molecules <i>Brassica oleracea</i> sulforaphane green extraction food waste antioxidants UPLC-ESI-MS |
| title | Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology |
| title_full | Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology |
| title_fullStr | Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology |
| title_full_unstemmed | Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology |
| title_short | Optimization of Ultrasound-Assisted Extraction of Glucosinolates from Upcycled Cauliflower Using Response Surface Methodology |
| title_sort | optimization of ultrasound assisted extraction of glucosinolates from upcycled cauliflower using response surface methodology |
| topic | <i>Brassica oleracea</i> sulforaphane green extraction food waste antioxidants UPLC-ESI-MS |
| url | https://www.mdpi.com/1420-3049/30/11/2326 |
| work_keys_str_mv | AT hanaderbewgedif optimizationofultrasoundassistedextractionofglucosinolatesfromupcycledcauliflowerusingresponsesurfacemethodology AT tessastatkie optimizationofultrasoundassistedextractionofglucosinolatesfromupcycledcauliflowerusingresponsesurfacemethodology AT joannatkaczewska optimizationofultrasoundassistedextractionofglucosinolatesfromupcycledcauliflowerusingresponsesurfacemethodology AT hpvasantharupasinghe optimizationofultrasoundassistedextractionofglucosinolatesfromupcycledcauliflowerusingresponsesurfacemethodology |