A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening
Abstract Extracellular matrix mimics are still needed to grow cancer cells in 3D environments and study their evolution in vitro while precisely controlling relevant features. Most models currently use collagen, which is biomimetic but degrades quickly, or artificial polymers, which can be chemicall...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-025-87896-7 |
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| author | Elliot Lopez-Vince Teresa Simon-Yarza Claire Wilhelm |
| author_facet | Elliot Lopez-Vince Teresa Simon-Yarza Claire Wilhelm |
| author_sort | Elliot Lopez-Vince |
| collection | DOAJ |
| description | Abstract Extracellular matrix mimics are still needed to grow cancer cells in 3D environments and study their evolution in vitro while precisely controlling relevant features. Most models currently use collagen, which is biomimetic but degrades quickly, or artificial polymers, which can be chemically modified but remain stiff. Herein we introduced a soft, non-adhesive, and resistant hydrogel platform for tumor spheroid production using a polysaccharide-based formulation. To ensure micro-structuring of the hydrogel and enable spheroid formation, 3D printed molds consisting of a network of 200-µm-diameter micropillars were used to generate microstructured hydrogel constructs that fit into a multi-well plate. This platform was validated for drug testing using three cancer cell lines (A673, MCF7 and U87) and 2 anticancer drugs (doxorubicin and paclitaxel). Drug response was assessed through bright-field microscopy monitoring and viability measurements after 48 h of treatment. This study validates the use of pullulan-dextran hydrogels for spheroid formation, combined with in situ drug screening. |
| format | Article |
| id | doaj-art-09c4df18130b4f638495fc81ece8a161 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-09c4df18130b4f638495fc81ece8a1612025-02-09T12:28:34ZengNature PortfolioScientific Reports2045-23222025-02-0115111310.1038/s41598-025-87896-7A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screeningElliot Lopez-Vince0Teresa Simon-Yarza1Claire Wilhelm2Université Paris Cité, Université Sorbonne Paris Nord, LVTS Inserm U1148Université Paris Cité, Université Sorbonne Paris Nord, LVTS Inserm U1148Laboratoire Physico Chimie Curie, PCC, Institut Curie, CNRS UMR168, Sorbonne University, PSL UniversityAbstract Extracellular matrix mimics are still needed to grow cancer cells in 3D environments and study their evolution in vitro while precisely controlling relevant features. Most models currently use collagen, which is biomimetic but degrades quickly, or artificial polymers, which can be chemically modified but remain stiff. Herein we introduced a soft, non-adhesive, and resistant hydrogel platform for tumor spheroid production using a polysaccharide-based formulation. To ensure micro-structuring of the hydrogel and enable spheroid formation, 3D printed molds consisting of a network of 200-µm-diameter micropillars were used to generate microstructured hydrogel constructs that fit into a multi-well plate. This platform was validated for drug testing using three cancer cell lines (A673, MCF7 and U87) and 2 anticancer drugs (doxorubicin and paclitaxel). Drug response was assessed through bright-field microscopy monitoring and viability measurements after 48 h of treatment. This study validates the use of pullulan-dextran hydrogels for spheroid formation, combined with in situ drug screening.https://doi.org/10.1038/s41598-025-87896-7HydrogelsIn vitro modelsTumor modelsSpheroidsBiomaterialsDrug screening |
| spellingShingle | Elliot Lopez-Vince Teresa Simon-Yarza Claire Wilhelm A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening Scientific Reports Hydrogels In vitro models Tumor models Spheroids Biomaterials Drug screening |
| title | A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening |
| title_full | A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening |
| title_fullStr | A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening |
| title_full_unstemmed | A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening |
| title_short | A polysaccharide-based hydrogel platform for tumor spheroid production and anticancer drug screening |
| title_sort | polysaccharide based hydrogel platform for tumor spheroid production and anticancer drug screening |
| topic | Hydrogels In vitro models Tumor models Spheroids Biomaterials Drug screening |
| url | https://doi.org/10.1038/s41598-025-87896-7 |
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