Identification and expression analysis of NUDIX gene family in Rosa chinensis ‘Old Blush’
To explore the function and potential application of NUDIX (nucleoside diphosphates linked to other moieties X) gene family in Rosa chinensis ‘Old Blush’, 41 NUDIX gene family members of Rosa chinensis ‘Old Blush’ were identified using bioinformatics methods, and their phylogenetic relationships, ge...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2024-12-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2023.08.251 |
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| Summary: | To explore the function and potential application of NUDIX (nucleoside diphosphates linked to other moieties X) gene family in Rosa chinensis ‘Old Blush’, 41 NUDIX gene family members of Rosa chinensis ‘Old Blush’ were identified using bioinformatics methods, and their phylogenetic relationships, gene structures, and expression patterns were analyzed as well. Phylogenetic tree of NUDIX was constructed from Rosa chinensis ‘Old Blush’, Rosa multiflora, Rosa rugosa, Rosa wichuraiana ‘Basye’s Thornless’, Fragaria vesca, Malus domestica, Arabidopsis thaliana and Oryza sativa. It was found that the NUDIX can be classified into 13 subfamilies, among which the number of members of NUDIX1, NUDIX2, NUDIX4, and NUDIX11 subfamilies was greater than that of the other subfamilies. Collinearity analysis of genes revealed that the whole genome duplication event occurred in NUDIX gene family of Rosa chinensis ‘Old Blush’, resulting in two pairs of collinear genes. The chromosome localization results indicated that NUDIX genes of Rosa chinensis ‘Old Blush’ underwent multiple tandem duplication event, most of which went through purifying selection. Transcriptomic data analysis revealed that RcNUDIX1a/1b/1d/1e were specifically expressed in petals. RcNUDIX1b/1e/4c/9/11d might be involved in the stress response in leaf, RcIDI might participate in regulating young root and early floral development in plant tissues, and RcNUDIX2b/2d/2h might participate in the regulation of flower organ senescence. In conclusion, the quantity amplification of NUDIX genes in Rosa chinensis ‘Old Blush’ was caused mainly by tandem duplication event. The NUDIX gene family might play an important role in physiological processes such as secondary metabolism, growth and development, and stress response in leaf of Rosa chinensis ‘Old Blush’, which provides a theoretical basis for further research on the function of NUDIX genes in Rosa chinensis ‘Old Blush’. |
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| ISSN: | 1008-9209 2097-5155 |