Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso
Sennaobtusifolia (L.) is a plant in the genus Senna that contributes to improving nutritional quality, food security, and better health protection for rural populations. However, very few studies have been devoted to it in Burkina Faso. Consequently, its genetic diversity remains poorly known. Such...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2023-01-01
|
| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2023/3761799 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849703035169669120 |
|---|---|
| author | Haoua Nacambo Nerbéwendé Sawadogo Teyiouê Benoit Joseph Batieno Mahamadi Hamed Ouedraogo Abdoul Kader Congo Wend-Pagnagde Felicien Marie Serge Zida Kiswendsida Romaric Nanema Mahamadou Sawadogo |
| author_facet | Haoua Nacambo Nerbéwendé Sawadogo Teyiouê Benoit Joseph Batieno Mahamadi Hamed Ouedraogo Abdoul Kader Congo Wend-Pagnagde Felicien Marie Serge Zida Kiswendsida Romaric Nanema Mahamadou Sawadogo |
| author_sort | Haoua Nacambo |
| collection | DOAJ |
| description | Sennaobtusifolia (L.) is a plant in the genus Senna that contributes to improving nutritional quality, food security, and better health protection for rural populations. However, very few studies have been devoted to it in Burkina Faso. Consequently, its genetic diversity remains poorly known. Such neglect would lead to the erosion of its genetic resource. The general objective of this study is to contribute to a better knowledge of the genetic diversity of the species in order to be able to issue scientific bases for its conservation, valorization, and genetic improvement. Sixty (60) accessions of Senna obtusifolia were collected in the wild from five provinces of three climatic zones of Burkina Faso. Molecular characterization was carried out using 18 SSR markers. Fifteen were polymorphic microsatellite markers leading one hundred and one (101) alleles in total, with an average of seven (7) alleles per locus. The number of effective alleles was 2.33. Expected heterozygosity, Shannon diversity index, and polymorphism information content averaged 0.47, 1.05, and 0.47. Molecular characterization revealed the existence of genetic diversity within the collection. This diversity has been structured into three genetic groups. Genetic group 3 presents the highest genetic diversity parameters. |
| format | Article |
| id | doaj-art-09c159335a6749ab889d2578067e59fc |
| institution | DOAJ |
| issn | 1537-744X |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-09c159335a6749ab889d2578067e59fc2025-08-20T03:17:26ZengWileyThe Scientific World Journal1537-744X2023-01-01202310.1155/2023/3761799Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina FasoHaoua Nacambo0Nerbéwendé Sawadogo1Teyiouê Benoit Joseph Batieno2Mahamadi Hamed Ouedraogo3Abdoul Kader Congo4Wend-Pagnagde Felicien Marie Serge Zida5Kiswendsida Romaric Nanema6Mahamadou Sawadogo7Equipe Génétique et Amélioration des plantesEquipe Génétique et Amélioration des plantesInstitut de l’Environnement et de Recherche Agricole (INERA)Equipe Génétique et Amélioration des plantesInstitut de l’Environnement et de Recherche Agricole (INERA)Institut de l’Environnement et de Recherche Agricole (INERA)Equipe Génétique et Amélioration des plantesEquipe Génétique et Amélioration des plantesSennaobtusifolia (L.) is a plant in the genus Senna that contributes to improving nutritional quality, food security, and better health protection for rural populations. However, very few studies have been devoted to it in Burkina Faso. Consequently, its genetic diversity remains poorly known. Such neglect would lead to the erosion of its genetic resource. The general objective of this study is to contribute to a better knowledge of the genetic diversity of the species in order to be able to issue scientific bases for its conservation, valorization, and genetic improvement. Sixty (60) accessions of Senna obtusifolia were collected in the wild from five provinces of three climatic zones of Burkina Faso. Molecular characterization was carried out using 18 SSR markers. Fifteen were polymorphic microsatellite markers leading one hundred and one (101) alleles in total, with an average of seven (7) alleles per locus. The number of effective alleles was 2.33. Expected heterozygosity, Shannon diversity index, and polymorphism information content averaged 0.47, 1.05, and 0.47. Molecular characterization revealed the existence of genetic diversity within the collection. This diversity has been structured into three genetic groups. Genetic group 3 presents the highest genetic diversity parameters.http://dx.doi.org/10.1155/2023/3761799 |
| spellingShingle | Haoua Nacambo Nerbéwendé Sawadogo Teyiouê Benoit Joseph Batieno Mahamadi Hamed Ouedraogo Abdoul Kader Congo Wend-Pagnagde Felicien Marie Serge Zida Kiswendsida Romaric Nanema Mahamadou Sawadogo Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso The Scientific World Journal |
| title | Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso |
| title_full | Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso |
| title_fullStr | Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso |
| title_full_unstemmed | Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso |
| title_short | Assessment of Genetic Diversity of a Collection of Senna obtusifolia (L.) Irwin and Barneby Using SSRs Markers in Burkina Faso |
| title_sort | assessment of genetic diversity of a collection of senna obtusifolia l irwin and barneby using ssrs markers in burkina faso |
| url | http://dx.doi.org/10.1155/2023/3761799 |
| work_keys_str_mv | AT haouanacambo assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT nerbewendesawadogo assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT teyiouebenoitjosephbatieno assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT mahamadihamedouedraogo assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT abdoulkadercongo assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT wendpagnagdefelicienmariesergezida assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT kiswendsidaromaricnanema assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso AT mahamadousawadogo assessmentofgeneticdiversityofacollectionofsennaobtusifolialirwinandbarnebyusingssrsmarkersinburkinafaso |