Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method
Given that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques wer...
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2025-02-01
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| author | Yuanqi Duan Jinfeng Sun Yongkang Xue Weiwei Xu Yuxin Jiang Tieqiang Zong Wei Zhou Zhengyu Hu Gao Li |
| author_facet | Yuanqi Duan Jinfeng Sun Yongkang Xue Weiwei Xu Yuxin Jiang Tieqiang Zong Wei Zhou Zhengyu Hu Gao Li |
| author_sort | Yuanqi Duan |
| collection | DOAJ |
| description | Given that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques were used to compare structural characteristics and morphology. Neuroprotective effects were explored using OGD/R-induced PC12 cells. The results showed that BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a had similar characteristic groups and contained both β-glycosidic and α-glycosidic bonds. Their molecular weights, in descending order, were 198.398 kDa, 184.690 kDa, 184.556 kDa, and 184.217 kDa, respectively. In addition, the four polysaccharides contained different proportions of glycosidic bonds, namely, Man<i>p</i>-(1→, →5)-Ara<i>f</i>-(1→, →3)-Gal<i>p</i> (or GalA<i>p</i>)-(1→, →4)-Glc<i>p</i>-(1→ and →3,6)-Gal<i>p</i>-(1→. BCP30-1a also contained a certain proportion of Gal<i>p</i>-(1→, and each polysaccharide had different microscopic characteristics and good thermal stability. Finally, BCP50-1a, BCP70-1a, and BCP90-1a exhibited good cytoprotective effects on PC12 cells based on the OGD/R model. These findings provide a novel regulatory strategy for the functional activity of BCPs and offer scientific evidence supporting application in the research field of ischemic stroke. |
| format | Article |
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| issn | 1420-3049 |
| language | English |
| publishDate | 2025-02-01 |
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| spelling | doaj-art-09b3da07138a4437854f96aec04bd71a2025-08-20T02:53:22ZengMDPI AGMolecules1420-30492025-02-0130599810.3390/molecules30050998Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation MethodYuanqi Duan0Jinfeng Sun1Yongkang Xue2Weiwei Xu3Yuxin Jiang4Tieqiang Zong5Wei Zhou6Zhengyu Hu7Gao Li8Key Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaKey Laboratory of Natural Medicines of the Changbai Mountain, Ministry of Education, College of Pharmacy, Yanbian University, Yanji 133002, ChinaGiven that the preparation method of polysaccharides affects the functional properties, four types of acidic polysaccharides (BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a) were prepared using the gradient ethanol precipitation method. Then, a series of chemical and instrumental analysis techniques were used to compare structural characteristics and morphology. Neuroprotective effects were explored using OGD/R-induced PC12 cells. The results showed that BCP30-1a, BCP50-1a, BCP70-1a, and BCP90-1a had similar characteristic groups and contained both β-glycosidic and α-glycosidic bonds. Their molecular weights, in descending order, were 198.398 kDa, 184.690 kDa, 184.556 kDa, and 184.217 kDa, respectively. In addition, the four polysaccharides contained different proportions of glycosidic bonds, namely, Man<i>p</i>-(1→, →5)-Ara<i>f</i>-(1→, →3)-Gal<i>p</i> (or GalA<i>p</i>)-(1→, →4)-Glc<i>p</i>-(1→ and →3,6)-Gal<i>p</i>-(1→. BCP30-1a also contained a certain proportion of Gal<i>p</i>-(1→, and each polysaccharide had different microscopic characteristics and good thermal stability. Finally, BCP50-1a, BCP70-1a, and BCP90-1a exhibited good cytoprotective effects on PC12 cells based on the OGD/R model. These findings provide a novel regulatory strategy for the functional activity of BCPs and offer scientific evidence supporting application in the research field of ischemic stroke.https://www.mdpi.com/1420-3049/30/5/998<i>Belamcanda chinensis</i> (L.) DC rhizomesgradient ethanol precipitation methodpolysaccharidesischemic stroke |
| spellingShingle | Yuanqi Duan Jinfeng Sun Yongkang Xue Weiwei Xu Yuxin Jiang Tieqiang Zong Wei Zhou Zhengyu Hu Gao Li Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method Molecules <i>Belamcanda chinensis</i> (L.) DC rhizomes gradient ethanol precipitation method polysaccharides ischemic stroke |
| title | Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method |
| title_full | Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method |
| title_fullStr | Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method |
| title_full_unstemmed | Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method |
| title_short | Physicochemical Properties and Cytoprotective Effects on PC12 Cells of Polysaccharides from <i>Belamcanda chinensis</i> (L.) DC. Obtained via a Gradient Ethanol Precipitation Method |
| title_sort | physicochemical properties and cytoprotective effects on pc12 cells of polysaccharides from i belamcanda chinensis i l dc obtained via a gradient ethanol precipitation method |
| topic | <i>Belamcanda chinensis</i> (L.) DC rhizomes gradient ethanol precipitation method polysaccharides ischemic stroke |
| url | https://www.mdpi.com/1420-3049/30/5/998 |
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