Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids.
The incorporation of the extracellular matrix (ECM) is essential for generating in vitro models that truly represent the microarchitecture found in human tissues. However, the cell-cell and cell-ECM interactions in vitro remains poorly understood in placental trophoblast biology. We investigated the...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199632&type=printable |
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| author | Michael K Wong Sarah A Shawky Aditya Aryasomayajula Madeline A Green Tom Ewart P Ravi Selvaganapathy Sandeep Raha |
| author_facet | Michael K Wong Sarah A Shawky Aditya Aryasomayajula Madeline A Green Tom Ewart P Ravi Selvaganapathy Sandeep Raha |
| author_sort | Michael K Wong |
| collection | DOAJ |
| description | The incorporation of the extracellular matrix (ECM) is essential for generating in vitro models that truly represent the microarchitecture found in human tissues. However, the cell-cell and cell-ECM interactions in vitro remains poorly understood in placental trophoblast biology. We investigated the effects of varying the surface properties (surface thickness and stiffness) of two ECMs, collagen I and Matrigel, on placental trophoblast cell morphology, viability, proliferation, and expression of markers involved in differentiation/syncytial fusion. Most notably, thicker Matrigel surfaces were found to induce the self-assembly of trophoblast cells into 3D spheroids that exhibited thickness-dependent changes in viability, proliferation, syncytial fusion, and gene expression profiles compared to two-dimensional cultures. Changes in F-actin organization, cell spread morphologies, and integrin and matrix metalloproteinase gene expression profiles, further reveal that the response to surface thickness may be mediated in part through cellular stiffness-sensing mechanisms. Our derivation of self-assembling trophoblast spheroid cultures through regulation of ECM surface alone contributes to a deeper understanding of cell-ECM interactions, and may be important for the advancement of in vitro platforms for research or diagnostics. |
| format | Article |
| id | doaj-art-09a853f2e2874eeeb420e759c0135f25 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-09a853f2e2874eeeb420e759c0135f252025-08-20T03:04:35ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019963210.1371/journal.pone.0199632Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids.Michael K WongSarah A ShawkyAditya AryasomayajulaMadeline A GreenTom EwartP Ravi SelvaganapathySandeep RahaThe incorporation of the extracellular matrix (ECM) is essential for generating in vitro models that truly represent the microarchitecture found in human tissues. However, the cell-cell and cell-ECM interactions in vitro remains poorly understood in placental trophoblast biology. We investigated the effects of varying the surface properties (surface thickness and stiffness) of two ECMs, collagen I and Matrigel, on placental trophoblast cell morphology, viability, proliferation, and expression of markers involved in differentiation/syncytial fusion. Most notably, thicker Matrigel surfaces were found to induce the self-assembly of trophoblast cells into 3D spheroids that exhibited thickness-dependent changes in viability, proliferation, syncytial fusion, and gene expression profiles compared to two-dimensional cultures. Changes in F-actin organization, cell spread morphologies, and integrin and matrix metalloproteinase gene expression profiles, further reveal that the response to surface thickness may be mediated in part through cellular stiffness-sensing mechanisms. Our derivation of self-assembling trophoblast spheroid cultures through regulation of ECM surface alone contributes to a deeper understanding of cell-ECM interactions, and may be important for the advancement of in vitro platforms for research or diagnostics.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199632&type=printable |
| spellingShingle | Michael K Wong Sarah A Shawky Aditya Aryasomayajula Madeline A Green Tom Ewart P Ravi Selvaganapathy Sandeep Raha Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. PLoS ONE |
| title | Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| title_full | Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| title_fullStr | Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| title_full_unstemmed | Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| title_short | Extracellular matrix surface regulates self-assembly of three-dimensional placental trophoblast spheroids. |
| title_sort | extracellular matrix surface regulates self assembly of three dimensional placental trophoblast spheroids |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0199632&type=printable |
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