Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan

Flavonoids, widely present in <i>Artemisia argyi</i> (AA), offer potential health benefits but are limited in food applications because of their bitter taste, inadequate absorption, and stability. Casein micelles encapsulation can enhance the flavonoid absorption, stability, and bioactiv...

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Main Authors: Ye Zhang, Dongliang Wang, Mengling Peng, Min Yang, Ya Yu, Mengting Yuan, Yanan Liu, Bingyu Zhu, Xiuheng Xue, Juhua Wang
Format: Article
Language:English
Published: MDPI AG 2025-07-01
Series:Foods
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Online Access:https://www.mdpi.com/2304-8158/14/14/2435
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author Ye Zhang
Dongliang Wang
Mengling Peng
Min Yang
Ya Yu
Mengting Yuan
Yanan Liu
Bingyu Zhu
Xiuheng Xue
Juhua Wang
author_facet Ye Zhang
Dongliang Wang
Mengling Peng
Min Yang
Ya Yu
Mengting Yuan
Yanan Liu
Bingyu Zhu
Xiuheng Xue
Juhua Wang
author_sort Ye Zhang
collection DOAJ
description Flavonoids, widely present in <i>Artemisia argyi</i> (AA), offer potential health benefits but are limited in food applications because of their bitter taste, inadequate absorption, and stability. Casein micelles encapsulation can enhance the flavonoid absorption, stability, and bioactivity. In this study, <i>Artemisia argyi</i> flavonoids (AAFs) were extracted using ultrasound-assisted extraction (UAE) to optimize the process. The glycosylation reaction between casein (CN) micelles and oat β-glucan (OBG) was employed to improve AAF’s emulsifying stability, sustained release during digestion, and cellular uptake. The maximum glycosylation degree of 32.33% was achieved at a CN-to-OBG ratio of 1:2, 120 min browning time, and 95 °C temperature. This glycosylated delivery system enhanced the emulsifying properties of the AAFs, digestive sustained release, and cellular uptake, showing potential as a cross-linking material for fat-soluble substances and medicines.
format Article
id doaj-art-09a2abcfb6ca4f3aa2fc41290a1af938
institution DOAJ
issn 2304-8158
language English
publishDate 2025-07-01
publisher MDPI AG
record_format Article
series Foods
spelling doaj-art-09a2abcfb6ca4f3aa2fc41290a1af9382025-08-20T02:45:45ZengMDPI AGFoods2304-81582025-07-011414243510.3390/foods14142435Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat GlucanYe Zhang0Dongliang Wang1Mengling Peng2Min Yang3Ya Yu4Mengting Yuan5Yanan Liu6Bingyu Zhu7Xiuheng Xue8Juhua Wang9College of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaSchool of Horticulture, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaSchool of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaSchool of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaSchool of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaSchool of Food and Nutrition, Anhui Agricultural University, Hefei 230036, ChinaCollege of Veterinary Medicine, Anhui Agricultural University, Hefei 230036, ChinaFlavonoids, widely present in <i>Artemisia argyi</i> (AA), offer potential health benefits but are limited in food applications because of their bitter taste, inadequate absorption, and stability. Casein micelles encapsulation can enhance the flavonoid absorption, stability, and bioactivity. In this study, <i>Artemisia argyi</i> flavonoids (AAFs) were extracted using ultrasound-assisted extraction (UAE) to optimize the process. The glycosylation reaction between casein (CN) micelles and oat β-glucan (OBG) was employed to improve AAF’s emulsifying stability, sustained release during digestion, and cellular uptake. The maximum glycosylation degree of 32.33% was achieved at a CN-to-OBG ratio of 1:2, 120 min browning time, and 95 °C temperature. This glycosylated delivery system enhanced the emulsifying properties of the AAFs, digestive sustained release, and cellular uptake, showing potential as a cross-linking material for fat-soluble substances and medicines.https://www.mdpi.com/2304-8158/14/14/2435<i>Artemisia argyi</i> flavonoidsglycosylation reactioncasein micellesemulsifying stabilitydigestive sustained release
spellingShingle Ye Zhang
Dongliang Wang
Mengling Peng
Min Yang
Ya Yu
Mengting Yuan
Yanan Liu
Bingyu Zhu
Xiuheng Xue
Juhua Wang
Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
Foods
<i>Artemisia argyi</i> flavonoids
glycosylation reaction
casein micelles
emulsifying stability
digestive sustained release
title Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
title_full Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
title_fullStr Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
title_full_unstemmed Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
title_short Emulsifying Stability, Digestive Sustained Release, and Cellular Uptake of Alcohol-Soluble <i>Artemisia argyi</i> Flavonoids Were Improved by Glycosylation of Casein Micelles with Oat Glucan
title_sort emulsifying stability digestive sustained release and cellular uptake of alcohol soluble i artemisia argyi i flavonoids were improved by glycosylation of casein micelles with oat glucan
topic <i>Artemisia argyi</i> flavonoids
glycosylation reaction
casein micelles
emulsifying stability
digestive sustained release
url https://www.mdpi.com/2304-8158/14/14/2435
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