Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam
Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013. Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified b...
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The Journal of Infection in Developing Countries
2016-09-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/7239 |
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| author | Huong Thi Thanh Doan Xuyen Thi Kim Le Roan Thi Do Chau Thi Minh Hoang Khue Thi Nguyen Thanh Hoa Le |
| author_facet | Huong Thi Thanh Doan Xuyen Thi Kim Le Roan Thi Do Chau Thi Minh Hoang Khue Thi Nguyen Thanh Hoa Le |
| author_sort | Huong Thi Thanh Doan |
| collection | DOAJ |
| description | Introduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013.
Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed.
Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%–100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%–100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains.
Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam.
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| format | Article |
| id | doaj-art-099eb4d0859c470e9ec62f9560ded13b |
| institution | OA Journals |
| issn | 1972-2680 |
| language | English |
| publishDate | 2016-09-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-099eb4d0859c470e9ec62f9560ded13b2025-08-20T02:16:06ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802016-09-01100910.3855/jidc.7239Molecular genotyping of duck hepatitis A viruses (DHAV) in VietnamHuong Thi Thanh Doan0Xuyen Thi Kim Le1Roan Thi Do2Chau Thi Minh Hoang3Khue Thi Nguyen4Thanh Hoa Le5Institute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamInstitute of Biotechnology, Vietnam Academy of Science and Technology, Hanoi, VietnamIntroduction: The aim of this study was to identify the genetic characteristics and molecular genotyping of duck hepatitis A virus (DHAV) isolated in Vietnam during 2009–2013. Methodology: Thirty duckling livers from outbreaks between 2009 and 2013 in seven provinces were collected and identified by polymerase chain reaction (PCR). Then, VP1 genes of eleven positive samples and two attenuated vaccine strains were sequenced and analyzed. Results: Genotypic and phylogenetic analyses indicated that the 13 Vietnamese isolates were classified into two genotypes, DHAV-1 and DHAV-3. The rate of identity and homology was 91%–100% between the 10 Vietnamese and 26 global strains of DHAV-3, and 92%–100% between 3 Vietnamese and 16 strains of DHAV-1. Between the DHAV-3 and DHAV-1 strains, the divergence reached 30%. At the C-terminal of VP1 for the different strains, a hypervariable region was observed, and notably, six of the Vietnamese DHAV-3 strains in this study showed four consistent differences (at positions T184M, Q200H, K207N, and K214R) within this group that were distinct from all other DHAV-3 strains. Conclusions: This is the first report of molecular characterization of DHAVs in Vietnam. At least two genotypes were identified, DHAV-1 and DHAV-3, with diversified clades within and between genotypes. DHAV-3 seemed to be dominant in Vietnam. https://jidc.org/index.php/journal/article/view/7239duck hepatitis A virusVP1genotypinginter/intragenotypic variationVietnam |
| spellingShingle | Huong Thi Thanh Doan Xuyen Thi Kim Le Roan Thi Do Chau Thi Minh Hoang Khue Thi Nguyen Thanh Hoa Le Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam Journal of Infection in Developing Countries duck hepatitis A virus VP1 genotyping inter/intragenotypic variation Vietnam |
| title | Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam |
| title_full | Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam |
| title_fullStr | Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam |
| title_full_unstemmed | Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam |
| title_short | Molecular genotyping of duck hepatitis A viruses (DHAV) in Vietnam |
| title_sort | molecular genotyping of duck hepatitis a viruses dhav in vietnam |
| topic | duck hepatitis A virus VP1 genotyping inter/intragenotypic variation Vietnam |
| url | https://jidc.org/index.php/journal/article/view/7239 |
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