Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress
Objectives Obesity is a worldwide public health problem, predisposing individuals to serious cardiovascular and metabolic complications such as type 2 diabetes mellitus. White adipose tissue serves as an important regulator of energy balance, and its expansion in obesity can trigger inflammatory rea...
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Taylor & Francis Group
2024-12-01
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| Series: | Redox Report |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/13510002.2024.2430882 |
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| author | Edina Bakondi Tobias Jung Susanna Marg Vanessa Schnell Daniela Weber Tim J. Schulz Tilman Grune Annika Höhn |
| author_facet | Edina Bakondi Tobias Jung Susanna Marg Vanessa Schnell Daniela Weber Tim J. Schulz Tilman Grune Annika Höhn |
| author_sort | Edina Bakondi |
| collection | DOAJ |
| description | Objectives Obesity is a worldwide public health problem, predisposing individuals to serious cardiovascular and metabolic complications such as type 2 diabetes mellitus. White adipose tissue serves as an important regulator of energy balance, and its expansion in obesity can trigger inflammatory reactions and oxidative stress, which can also lead to insulin resistance. Adipocytes, with a key role in regulating metabolic homeostasis, respond to increased calorie intake and altered fatty acid composition with hypertrophy or hyperplasia. Of particular interest are saturated fatty acids such as palmitic acid and omega-3 polyunsaturated fatty acids such as eicosapentaenoic acid (EPA), which have differential effects on adipocyte function and inflammation.Methods Using 3T3-L1 cells as a model for adipocytes, we evaluated the effects of PA and EPA on lipid accumulation, droplet size, and oxidative stress markers.Results We were able to show that EPA supplementation in 3T3 adipocytes does not lead to excessive lipid accumulation, but rather reduces the size of lipid droplets and also induces redox changes due to the unsaturated nature of EPA.Discussion These results emphasize the contrasting roles of PA and EPA and the importance of fatty acid composition in the regulation of adipocyte function. |
| format | Article |
| id | doaj-art-0997bbde4e894726b8c3a3be12e849be |
| institution | OA Journals |
| issn | 1351-0002 1743-2928 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Redox Report |
| spelling | doaj-art-0997bbde4e894726b8c3a3be12e849be2025-08-20T02:21:07ZengTaylor & Francis GroupRedox Report1351-00021743-29282024-12-0129110.1080/13510002.2024.2430882Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stressEdina Bakondi0Tobias Jung1Susanna Marg2Vanessa Schnell3Daniela Weber4Tim J. Schulz5Tilman Grune6Annika Höhn7Department of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Adipocyte Development and Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Adipocyte Development and Nutrition, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyDepartment of Molecular Toxicology, German Institute of Human Nutrition Potsdam-Rehbruecke, Nuthetal, GermanyObjectives Obesity is a worldwide public health problem, predisposing individuals to serious cardiovascular and metabolic complications such as type 2 diabetes mellitus. White adipose tissue serves as an important regulator of energy balance, and its expansion in obesity can trigger inflammatory reactions and oxidative stress, which can also lead to insulin resistance. Adipocytes, with a key role in regulating metabolic homeostasis, respond to increased calorie intake and altered fatty acid composition with hypertrophy or hyperplasia. Of particular interest are saturated fatty acids such as palmitic acid and omega-3 polyunsaturated fatty acids such as eicosapentaenoic acid (EPA), which have differential effects on adipocyte function and inflammation.Methods Using 3T3-L1 cells as a model for adipocytes, we evaluated the effects of PA and EPA on lipid accumulation, droplet size, and oxidative stress markers.Results We were able to show that EPA supplementation in 3T3 adipocytes does not lead to excessive lipid accumulation, but rather reduces the size of lipid droplets and also induces redox changes due to the unsaturated nature of EPA.Discussion These results emphasize the contrasting roles of PA and EPA and the importance of fatty acid composition in the regulation of adipocyte function.https://www.tandfonline.com/doi/10.1080/13510002.2024.2430882Obesityadipocytesfatty acidslipid dropletsoxidative stresseicosapentaenoic acid |
| spellingShingle | Edina Bakondi Tobias Jung Susanna Marg Vanessa Schnell Daniela Weber Tim J. Schulz Tilman Grune Annika Höhn Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress Redox Report Obesity adipocytes fatty acids lipid droplets oxidative stress eicosapentaenoic acid |
| title | Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress |
| title_full | Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress |
| title_fullStr | Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress |
| title_full_unstemmed | Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress |
| title_short | Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress |
| title_sort | palmitic acid and eicosapentaenoic acid supplementation in 3t3 adipocytes impact on lipid storage and oxidative stress |
| topic | Obesity adipocytes fatty acids lipid droplets oxidative stress eicosapentaenoic acid |
| url | https://www.tandfonline.com/doi/10.1080/13510002.2024.2430882 |
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