Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou
The present study aimed to characterize the chromosome features of cherry (Prunus pseudocerasus Lindl.) germplasm in Guizhou Province, China, in order to facilitate the selection and breeding programs of this economically and ornamentally valuable species. The stem tip chromosome preparation techniq...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
PeerJ Inc.
2024-12-01
|
| Series: | PeerJ |
| Subjects: | |
| Online Access: | https://peerj.com/articles/18668.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850250928831070208 |
|---|---|
| author | Nian Chen Yali Wang Mei He Fei An Jiyue Wang Changmei Song |
| author_facet | Nian Chen Yali Wang Mei He Fei An Jiyue Wang Changmei Song |
| author_sort | Nian Chen |
| collection | DOAJ |
| description | The present study aimed to characterize the chromosome features of cherry (Prunus pseudocerasus Lindl.) germplasm in Guizhou Province, China, in order to facilitate the selection and breeding programs of this economically and ornamentally valuable species. The stem tip chromosome preparation technique was employed for ploidy identification and karyotype analysis, and the results were further validated by flow cytometry. The findings revealed that the 28 cherry accessions from Guizhou province exhibited a chromosomal base of x = 8, comprising 19 tetraploid and nine hexaploid individuals. Karyotype analysis showed two chromosome types, “m” and “sm,” with the longest chromosome/shortest chromosome (Lc/Sc) ranging from 1.6 to 2.65, the mean arm ratio (MAR) varying from 1.15 to 1.56, and index of the karyotypic asymmetry (As.K) ranging from 53.74 to 61.6. Three karyotype types, “1A,” “1B,” and “2B,” were identified among the studied accessions. The most evolutionarily advanced accession was HZ152, while DCZC27 represented the most primitive karyotype. This study expands the ploidy database of Chinese cherry and provides valuable information for the conservation and utilization of cherry germplasm resources in Guizhou province. |
| format | Article |
| id | doaj-art-096c04e4746b445eaef2e58dfdbf9d66 |
| institution | OA Journals |
| issn | 2167-8359 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj-art-096c04e4746b445eaef2e58dfdbf9d662025-08-20T01:58:04ZengPeerJ Inc.PeerJ2167-83592024-12-0112e1866810.7717/peerj.18668Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in GuizhouNian ChenYali WangMei HeFei AnJiyue WangChangmei SongThe present study aimed to characterize the chromosome features of cherry (Prunus pseudocerasus Lindl.) germplasm in Guizhou Province, China, in order to facilitate the selection and breeding programs of this economically and ornamentally valuable species. The stem tip chromosome preparation technique was employed for ploidy identification and karyotype analysis, and the results were further validated by flow cytometry. The findings revealed that the 28 cherry accessions from Guizhou province exhibited a chromosomal base of x = 8, comprising 19 tetraploid and nine hexaploid individuals. Karyotype analysis showed two chromosome types, “m” and “sm,” with the longest chromosome/shortest chromosome (Lc/Sc) ranging from 1.6 to 2.65, the mean arm ratio (MAR) varying from 1.15 to 1.56, and index of the karyotypic asymmetry (As.K) ranging from 53.74 to 61.6. Three karyotype types, “1A,” “1B,” and “2B,” were identified among the studied accessions. The most evolutionarily advanced accession was HZ152, while DCZC27 represented the most primitive karyotype. This study expands the ploidy database of Chinese cherry and provides valuable information for the conservation and utilization of cherry germplasm resources in Guizhou province.https://peerj.com/articles/18668.pdfPrunus pseudocerasus Lindl.Chromosome preparation techniquesKaryotypingFlow cytometry |
| spellingShingle | Nian Chen Yali Wang Mei He Fei An Jiyue Wang Changmei Song Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou PeerJ Prunus pseudocerasus Lindl. Chromosome preparation techniques Karyotyping Flow cytometry |
| title | Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou |
| title_full | Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou |
| title_fullStr | Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou |
| title_full_unstemmed | Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou |
| title_short | Identification of chromosome ploidy and karyotype analysis of cherries (Prunus pseudocerasus Lindl.) in Guizhou |
| title_sort | identification of chromosome ploidy and karyotype analysis of cherries prunus pseudocerasus lindl in guizhou |
| topic | Prunus pseudocerasus Lindl. Chromosome preparation techniques Karyotyping Flow cytometry |
| url | https://peerj.com/articles/18668.pdf |
| work_keys_str_mv | AT nianchen identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou AT yaliwang identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou AT meihe identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou AT feian identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou AT jiyuewang identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou AT changmeisong identificationofchromosomeploidyandkaryotypeanalysisofcherriesprunuspseudocerasuslindlinguizhou |