Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages
Objective To investigate the effect of lncRNA-Scarna10 (Scarna10) on resveratrol (RSV)-induced polarization of macrophages. Methods M1 polarization model of RAW264.7 macrophages was established by LPS and treated with RSV for 24 hours. The study groups were divided into control group, LPS group and...
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Editorial Office of Journal of Guangxi Medical University
2024-08-01
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| Series: | Guangxi Yike Daxue xuebao |
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| Online Access: | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.003 |
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| author | LUO Jianming JING Jie LIANG Yaodan ZHANG Taicheng CHENG Dongyu JIANG Haixing QIN Shanyu |
| author_facet | LUO Jianming JING Jie LIANG Yaodan ZHANG Taicheng CHENG Dongyu JIANG Haixing QIN Shanyu |
| author_sort | LUO Jianming |
| collection | DOAJ |
| description | Objective To investigate the effect of lncRNA-Scarna10 (Scarna10) on resveratrol (RSV)-induced polarization of macrophages. Methods M1 polarization model of RAW264.7 macrophages was established by LPS and treated with RSV for 24 hours. The study groups were divided into control group, LPS group and RSV+ LPS group. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of Scarna10, and western blotting and immunofluorescence were used to detect the expression of macrophage polarization markers (iNOS, Arg-1, CD206). RAW264.7 cells were divided into Si-NC group, LPS+Si-NC group, RSV+LPS+Si-NC group and RSV+LPS+Si-Scarna10 group after silencing Scarna10, and the expression of Scarna10 and macrophage polarization markers in each group after transfection was detected. Results After LPS induction and resveratrol intervention, the protein expression level of iNOS, a M1 polarization marker, was elevated in the LPS group, the protein expression levels of Arg-1 and CD206, M2 polarization markers, were elevated, while the protein expression level of iNOS was decreased in the RSV+LPS group. At the same time, the expression level of Scarna10 in the RSV+LPS group was higher than that in the LPS group. After silencing Scarna10, compared with the RSV+LPS+Si-NC group, the expression level of Scarna10 and the protein expression levels of Arg-1 and CD206 in RSV+LPS+Si-Scarna10 group were decreased, and the protein expression level of iNOS was elevated. The differences among the above groups were statistically significant (all P < 0.05). Conclusion The expression of Scarna10 is upregulated in M1 macrophages following RSV intervention, and silencing Scarna10 can reverse the RSV-induced promotion of M1-to-M2 macrophage polarization. |
| format | Article |
| id | doaj-art-0968cae7f48d49008b35f4f0189fb813 |
| institution | DOAJ |
| issn | 1005-930X |
| language | zho |
| publishDate | 2024-08-01 |
| publisher | Editorial Office of Journal of Guangxi Medical University |
| record_format | Article |
| series | Guangxi Yike Daxue xuebao |
| spelling | doaj-art-0968cae7f48d49008b35f4f0189fb8132025-08-20T02:57:28ZzhoEditorial Office of Journal of Guangxi Medical UniversityGuangxi Yike Daxue xuebao1005-930X2024-08-014181120112610.16190/j.cnki.45-1211/r.2024.08.003gxykdxxb-41-8-1120Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophagesLUO Jianming0JING Jie1LIANG Yaodan2ZHANG Taicheng3CHENG Dongyu4JIANG Haixing5QIN Shanyu6Department of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaDepartment of Gastroenterology, the First Affiliated Hospital of Guangxi Medical University, Nanning 530021, ChinaObjective To investigate the effect of lncRNA-Scarna10 (Scarna10) on resveratrol (RSV)-induced polarization of macrophages. Methods M1 polarization model of RAW264.7 macrophages was established by LPS and treated with RSV for 24 hours. The study groups were divided into control group, LPS group and RSV+ LPS group. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression of Scarna10, and western blotting and immunofluorescence were used to detect the expression of macrophage polarization markers (iNOS, Arg-1, CD206). RAW264.7 cells were divided into Si-NC group, LPS+Si-NC group, RSV+LPS+Si-NC group and RSV+LPS+Si-Scarna10 group after silencing Scarna10, and the expression of Scarna10 and macrophage polarization markers in each group after transfection was detected. Results After LPS induction and resveratrol intervention, the protein expression level of iNOS, a M1 polarization marker, was elevated in the LPS group, the protein expression levels of Arg-1 and CD206, M2 polarization markers, were elevated, while the protein expression level of iNOS was decreased in the RSV+LPS group. At the same time, the expression level of Scarna10 in the RSV+LPS group was higher than that in the LPS group. After silencing Scarna10, compared with the RSV+LPS+Si-NC group, the expression level of Scarna10 and the protein expression levels of Arg-1 and CD206 in RSV+LPS+Si-Scarna10 group were decreased, and the protein expression level of iNOS was elevated. The differences among the above groups were statistically significant (all P < 0.05). Conclusion The expression of Scarna10 is upregulated in M1 macrophages following RSV intervention, and silencing Scarna10 can reverse the RSV-induced promotion of M1-to-M2 macrophage polarization.https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.003lncrna-scarna10resveratrolmacrophage polarization |
| spellingShingle | LUO Jianming JING Jie LIANG Yaodan ZHANG Taicheng CHENG Dongyu JIANG Haixing QIN Shanyu Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages Guangxi Yike Daxue xuebao lncrna-scarna10 resveratrol macrophage polarization |
| title | Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages |
| title_full | Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages |
| title_fullStr | Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages |
| title_full_unstemmed | Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages |
| title_short | Experimental study on the effect of lncRNA-Scarna10 on resveratrol-induced M2 polarization of macrophages |
| title_sort | experimental study on the effect of lncrna scarna10 on resveratrol induced m2 polarization of macrophages |
| topic | lncrna-scarna10 resveratrol macrophage polarization |
| url | https://journal.gxmu.edu.cn/article/doi/10.16190/j.cnki.45-1211/r.2024.08.003 |
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