Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica

Biochemical investigations were carried out to reveal the solvent differential as it affects the antioxidant properties and gas chromatography mass spectrometry (GC-MS) analysis of Monodora myristica. The M. myristica seeds were sun-dried for two weeks to obtain constant weight and then crushed into...

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Main Author: Okpoghono, J.*, Achuba, F.I. and George, B.O.
Format: Article
Language:English
Published: Hammer Head Production Limited 2018-03-01
Series:Sokoto Journal of Medical Laboratory Science
Subjects:
Online Access:https://sokjmls.com.ng/index.php/SJMLS/article/view/290
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author Okpoghono, J.*, Achuba, F.I. and George, B.O.
author_facet Okpoghono, J.*, Achuba, F.I. and George, B.O.
author_sort Okpoghono, J.*, Achuba, F.I. and George, B.O.
collection DOAJ
description Biochemical investigations were carried out to reveal the solvent differential as it affects the antioxidant properties and gas chromatography mass spectrometry (GC-MS) analysis of Monodora myristica. The M. myristica seeds were sun-dried for two weeks to obtain constant weight and then crushed into fine particles using electric blender. Extraction was done using water (60 0C), ethanol and diethyl ether.  Biochemical analyses were carried out using standard methods. The results of the phytochemical screening of M. myristica crude sample indicate the presence of saponin (4.25 %), tannin (0.53 %), alkaloid (3.82 %), flavonoid (3.47 %).  The total phenolic content, ascorbate oxidase and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of M. myristica extracts were as follows; M. myristica diethyl ether extract (MDEE) > M. myristica ethanol extract (MEE) > M. myristica water extract (MWE). The gas chromatography mass spectrometry (GCMS) analysis showed the presence of twelve compounds in MDEE, eight compounds from MEE and six compounds were obtained from MWE.  It could be concluded that M. myristica   seeds possess antioxidant properties and contains various phytochemical compounds. This may confirm the traditional uses of M. myristica seeds as an antioxidant of nutritional significance.
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spelling doaj-art-09571a317ee4466dbf51c2c244d028a62025-08-20T03:41:46ZengHammer Head Production LimitedSokoto Journal of Medical Laboratory Science2536-71532018-03-0131290Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristicaOkpoghono, J.*, Achuba, F.I. and George, B.O.Biochemical investigations were carried out to reveal the solvent differential as it affects the antioxidant properties and gas chromatography mass spectrometry (GC-MS) analysis of Monodora myristica. The M. myristica seeds were sun-dried for two weeks to obtain constant weight and then crushed into fine particles using electric blender. Extraction was done using water (60 0C), ethanol and diethyl ether.  Biochemical analyses were carried out using standard methods. The results of the phytochemical screening of M. myristica crude sample indicate the presence of saponin (4.25 %), tannin (0.53 %), alkaloid (3.82 %), flavonoid (3.47 %).  The total phenolic content, ascorbate oxidase and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activity of M. myristica extracts were as follows; M. myristica diethyl ether extract (MDEE) > M. myristica ethanol extract (MEE) > M. myristica water extract (MWE). The gas chromatography mass spectrometry (GCMS) analysis showed the presence of twelve compounds in MDEE, eight compounds from MEE and six compounds were obtained from MWE.  It could be concluded that M. myristica   seeds possess antioxidant properties and contains various phytochemical compounds. This may confirm the traditional uses of M. myristica seeds as an antioxidant of nutritional significance.https://sokjmls.com.ng/index.php/SJMLS/article/view/290monodora myristica, antioxidant, water, ethanol, diethyl ether.
spellingShingle Okpoghono, J.*, Achuba, F.I. and George, B.O.
Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
Sokoto Journal of Medical Laboratory Science
monodora myristica, antioxidant, water, ethanol, diethyl ether.
title Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
title_full Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
title_fullStr Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
title_full_unstemmed Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
title_short Differential Solvent Extraction on The Antioxidant Properties, and Phytochemical Compounds of Monodora myristica
title_sort differential solvent extraction on the antioxidant properties and phytochemical compounds of monodora myristica
topic monodora myristica, antioxidant, water, ethanol, diethyl ether.
url https://sokjmls.com.ng/index.php/SJMLS/article/view/290
work_keys_str_mv AT okpoghonojachubafiandgeorgebo differentialsolventextractionontheantioxidantpropertiesandphytochemicalcompoundsofmonodoramyristica