Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway
The activity of a local renin-angiotensin system (RAS) in the adipose tissue is closely associated with obesity-related diseases. However, the mechanism of RAS activation in adipose tissue is still unknown. In the current study, we found that palmitic acid (PA), one kind of free fatty acid, induced...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2016/1587594 |
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| author | Jia Sun Jinhua Luo Yuting Ruan Liangchang Xiu Bimei Fang Hua Zhang Ming Wang Hong Chen |
| author_facet | Jia Sun Jinhua Luo Yuting Ruan Liangchang Xiu Bimei Fang Hua Zhang Ming Wang Hong Chen |
| author_sort | Jia Sun |
| collection | DOAJ |
| description | The activity of a local renin-angiotensin system (RAS) in the adipose tissue is closely associated with obesity-related diseases. However, the mechanism of RAS activation in adipose tissue is still unknown. In the current study, we found that palmitic acid (PA), one kind of free fatty acid, induced the activity of RAS in 3T3-L1 adipocytes. In the presence of fetuin A (Fet A), PA upregulated the expression of angiotensinogen (AGT) and angiotensin type 1 receptor (AT1R) and stimulated the secretion of angiotensin II (ANG II) in 3T3-L1 adipocytes. Moreover, the activation of RAS in 3T3-L1 adipocytes was blocked when we blocked Toll-like receptor 4 (TLR4) signaling pathway using TAK242 or NF-κB signaling pathway using BAY117082. Together, our results have identified critical molecular mechanisms linking PA/TLR4/NF-κB signaling pathway to the activity of the local renin-angiotensin system in adipose tissue. |
| format | Article |
| id | doaj-art-094ffe350b574ff7bf22490dda13df73 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-094ffe350b574ff7bf22490dda13df732025-02-03T05:54:39ZengWileyJournal of Diabetes Research2314-67452314-67532016-01-01201610.1155/2016/15875941587594Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B PathwayJia Sun0Jinhua Luo1Yuting Ruan2Liangchang Xiu3Bimei Fang4Hua Zhang5Ming Wang6Hong Chen7Department of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Geratology, The Affiliated Hospital of Guangdong Medical College, Guangdong Medical College, Zhanjiang, Guangdong, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Epidemiology and Medical Statistics, School of Public Health, Guangdong Medical College, Dongguan, Guangdong, ChinaSecond Clinical School of Medicine, Southern Medical University, Guangzhou, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaNephrology Center of Integrated Traditional Chinese and Western Medicine, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaDepartment of Endocrinology, Zhujiang Hospital, Southern Medical University, Guangzhou, ChinaThe activity of a local renin-angiotensin system (RAS) in the adipose tissue is closely associated with obesity-related diseases. However, the mechanism of RAS activation in adipose tissue is still unknown. In the current study, we found that palmitic acid (PA), one kind of free fatty acid, induced the activity of RAS in 3T3-L1 adipocytes. In the presence of fetuin A (Fet A), PA upregulated the expression of angiotensinogen (AGT) and angiotensin type 1 receptor (AT1R) and stimulated the secretion of angiotensin II (ANG II) in 3T3-L1 adipocytes. Moreover, the activation of RAS in 3T3-L1 adipocytes was blocked when we blocked Toll-like receptor 4 (TLR4) signaling pathway using TAK242 or NF-κB signaling pathway using BAY117082. Together, our results have identified critical molecular mechanisms linking PA/TLR4/NF-κB signaling pathway to the activity of the local renin-angiotensin system in adipose tissue.http://dx.doi.org/10.1155/2016/1587594 |
| spellingShingle | Jia Sun Jinhua Luo Yuting Ruan Liangchang Xiu Bimei Fang Hua Zhang Ming Wang Hong Chen Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway Journal of Diabetes Research |
| title | Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway |
| title_full | Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway |
| title_fullStr | Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway |
| title_full_unstemmed | Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway |
| title_short | Free Fatty Acids Activate Renin-Angiotensin System in 3T3-L1 Adipocytes through Nuclear Factor-kappa B Pathway |
| title_sort | free fatty acids activate renin angiotensin system in 3t3 l1 adipocytes through nuclear factor kappa b pathway |
| url | http://dx.doi.org/10.1155/2016/1587594 |
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