Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites
This study evaluated the microbial growth profile of subgingival multispecies biofilm on 3D-printable resin-based composites (PRBCs). A 96-well cell plate cultivated a 39-species biofilm associated with periodontitis over 7 days. Cylindrical specimens with 12 mm high and 3 mm diameters were prepared...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Journal of Functional Biomaterials |
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| Online Access: | https://www.mdpi.com/2079-4983/16/1/12 |
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| author | Emerson Koji Uehara Gustavo Castro de Lima Janaina de Cassia Orlandi Sardi Luciene Cristina de Figueiredo Jamil Awad Shibli Thabet Asbi Doron Haim José Augusto Rodrigues |
| author_facet | Emerson Koji Uehara Gustavo Castro de Lima Janaina de Cassia Orlandi Sardi Luciene Cristina de Figueiredo Jamil Awad Shibli Thabet Asbi Doron Haim José Augusto Rodrigues |
| author_sort | Emerson Koji Uehara |
| collection | DOAJ |
| description | This study evaluated the microbial growth profile of subgingival multispecies biofilm on 3D-printable resin-based composites (PRBCs). A 96-well cell plate cultivated a 39-species biofilm associated with periodontitis over 7 days. Cylindrical specimens with 12 mm high and 3 mm diameters were prepared by the PRBC group (Cosmos Temp-Yller; Prizma 3D Bio Crown; Prizma 3D Bio Prov) and an acrylic resin as control. Further, these specimens were immersed in the well plate to allow biofilm formation. After growing for 7 days, the metabolic biofilm activity was evaluated by colorimetric assay and the microbial profile by DNA-DNA hybridization. Kruskal–Wallis and Mann–Whitney tests evaluated each bacteria count and complex group. A greater biofilm formation was observed on PRBC groups than on acrylic resin. The microbiological profile of PRBC was associated with a less pathogenic biofilm, with an absence of a red complex. Acrylic resin showed low biofilm growth, but the biofilm profile was related to periodontal disease, characterized by red-complex bacteria. The selection of PRBC may contribute more effectively to maintaining periodontal health than acrylic resin. |
| format | Article |
| id | doaj-art-094dfc702b1546728da88462b7f6585d |
| institution | Kabale University |
| issn | 2079-4983 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Functional Biomaterials |
| spelling | doaj-art-094dfc702b1546728da88462b7f6585d2025-01-24T13:36:06ZengMDPI AGJournal of Functional Biomaterials2079-49832025-01-011611210.3390/jfb16010012Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based CompositesEmerson Koji Uehara0Gustavo Castro de Lima1Janaina de Cassia Orlandi Sardi2Luciene Cristina de Figueiredo3Jamil Awad Shibli4Thabet Asbi5Doron Haim6José Augusto Rodrigues7Dental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilMaccabi-Dent Research Department, Tel-Aviv 6801298, IsraelDental Research Division, Guarulhos University, Guarulhos 07023-070, SP, BrazilThis study evaluated the microbial growth profile of subgingival multispecies biofilm on 3D-printable resin-based composites (PRBCs). A 96-well cell plate cultivated a 39-species biofilm associated with periodontitis over 7 days. Cylindrical specimens with 12 mm high and 3 mm diameters were prepared by the PRBC group (Cosmos Temp-Yller; Prizma 3D Bio Crown; Prizma 3D Bio Prov) and an acrylic resin as control. Further, these specimens were immersed in the well plate to allow biofilm formation. After growing for 7 days, the metabolic biofilm activity was evaluated by colorimetric assay and the microbial profile by DNA-DNA hybridization. Kruskal–Wallis and Mann–Whitney tests evaluated each bacteria count and complex group. A greater biofilm formation was observed on PRBC groups than on acrylic resin. The microbiological profile of PRBC was associated with a less pathogenic biofilm, with an absence of a red complex. Acrylic resin showed low biofilm growth, but the biofilm profile was related to periodontal disease, characterized by red-complex bacteria. The selection of PRBC may contribute more effectively to maintaining periodontal health than acrylic resin.https://www.mdpi.com/2079-4983/16/1/12periodontal pathogensantimicrobial activityperiodontal disease3D printed resinsadditive manufacturingcomputer-aided design |
| spellingShingle | Emerson Koji Uehara Gustavo Castro de Lima Janaina de Cassia Orlandi Sardi Luciene Cristina de Figueiredo Jamil Awad Shibli Thabet Asbi Doron Haim José Augusto Rodrigues Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites Journal of Functional Biomaterials periodontal pathogens antimicrobial activity periodontal disease 3D printed resins additive manufacturing computer-aided design |
| title | Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites |
| title_full | Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites |
| title_fullStr | Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites |
| title_full_unstemmed | Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites |
| title_short | Next-Generation Dental Materials: Exploring Bacterial Biofilm Formation on 3D-Printable Resin-Based Composites |
| title_sort | next generation dental materials exploring bacterial biofilm formation on 3d printable resin based composites |
| topic | periodontal pathogens antimicrobial activity periodontal disease 3D printed resins additive manufacturing computer-aided design |
| url | https://www.mdpi.com/2079-4983/16/1/12 |
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