In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea
Apocynum lancifolium Russanov is an endangered and ecologically important plant endemic to South Korea. However, neither the mass germination of Apocynum seeds nor the production of its plantlets using in vitro culture systems have been reported. Therefore, we conducted in vitro experiments to inves...
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Taylor & Francis Group
2025-04-01
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| Series: | Forest Science and Technology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21580103.2025.2463485 |
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| author | Ye Joo Sol Da Hyun Lee Ye Eun Kim Cho Rong Park Jung Min Kim Chang Ho Ahn |
| author_facet | Ye Joo Sol Da Hyun Lee Ye Eun Kim Cho Rong Park Jung Min Kim Chang Ho Ahn |
| author_sort | Ye Joo Sol |
| collection | DOAJ |
| description | Apocynum lancifolium Russanov is an endangered and ecologically important plant endemic to South Korea. However, neither the mass germination of Apocynum seeds nor the production of its plantlets using in vitro culture systems have been reported. Therefore, we conducted in vitro experiments to investigate the effects of temperature on seed germination inoculated onto half-strength MS medium. Additionally, we described the mass proliferation of A. lancifolium by using an in vitro stem cutting propagation system. The effect of temperature on A. lancifolium seed germination was significant (p = 0.018). A. lancifolium germinated 2 d after inoculation, with germination rates of 64%, 54%, 50%, and 35% at 25, 20, 15/25, and 15 °C, respectively. A. lancifolium seeds inoculated at 25 °C had the highest germination rate (64 ± 4.85%) and daily mean for in vitro propagation and regeneration. A half-strength WPM was more efficient than any other salt composition (MS and DKW) for the growth of A. lancifolium, although the effects of three different basal culture media on the growth of A. lancifolium were not significant. This in vitro germination, propagation, and regeneration system for A. lancifolium provides a means to substantially advance A. lancifolium germplasm conservation and restoration. |
| format | Article |
| id | doaj-art-0944d7defb7841ea939185aa4e244343 |
| institution | Kabale University |
| issn | 2158-0103 2158-0715 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Taylor & Francis Group |
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| series | Forest Science and Technology |
| spelling | doaj-art-0944d7defb7841ea939185aa4e2443432025-08-20T03:45:19ZengTaylor & Francis GroupForest Science and Technology2158-01032158-07152025-04-0121213113710.1080/21580103.2025.2463485In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South KoreaYe Joo Sol0Da Hyun Lee1Ye Eun Kim2Cho Rong Park3Jung Min Kim4Chang Ho Ahn5Gyeonggi Province Forestry, Badahyanggi Arboretum, Ansan-si, Republic of KoreaForest Biological Resource Department, Baekdudaegan National Arboretum, Korea Arboreta and Gardens Institute, Bonghwa-gun, Republic of KoreaDivision of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong-si, Republic of KoreaDivision of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong-si, Republic of KoreaDivision of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong-si, Republic of KoreaDivision of Garden Material Research, Sejong National Arboretum, Korea Arboreta and Gardens Institute, Sejong-si, Republic of KoreaApocynum lancifolium Russanov is an endangered and ecologically important plant endemic to South Korea. However, neither the mass germination of Apocynum seeds nor the production of its plantlets using in vitro culture systems have been reported. Therefore, we conducted in vitro experiments to investigate the effects of temperature on seed germination inoculated onto half-strength MS medium. Additionally, we described the mass proliferation of A. lancifolium by using an in vitro stem cutting propagation system. The effect of temperature on A. lancifolium seed germination was significant (p = 0.018). A. lancifolium germinated 2 d after inoculation, with germination rates of 64%, 54%, 50%, and 35% at 25, 20, 15/25, and 15 °C, respectively. A. lancifolium seeds inoculated at 25 °C had the highest germination rate (64 ± 4.85%) and daily mean for in vitro propagation and regeneration. A half-strength WPM was more efficient than any other salt composition (MS and DKW) for the growth of A. lancifolium, although the effects of three different basal culture media on the growth of A. lancifolium were not significant. This in vitro germination, propagation, and regeneration system for A. lancifolium provides a means to substantially advance A. lancifolium germplasm conservation and restoration.https://www.tandfonline.com/doi/10.1080/21580103.2025.2463485Apocynaceaedogbaneseedin vitro cultureWoody Plant Medium |
| spellingShingle | Ye Joo Sol Da Hyun Lee Ye Eun Kim Cho Rong Park Jung Min Kim Chang Ho Ahn In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea Forest Science and Technology Apocynaceae dogbane seed in vitro culture Woody Plant Medium |
| title | In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea |
| title_full | In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea |
| title_fullStr | In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea |
| title_full_unstemmed | In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea |
| title_short | In vitro germination and propagation of Apocynum lancifolium Russanov, an endemic species in South Korea |
| title_sort | in vitro germination and propagation of apocynum lancifolium russanov an endemic species in south korea |
| topic | Apocynaceae dogbane seed in vitro culture Woody Plant Medium |
| url | https://www.tandfonline.com/doi/10.1080/21580103.2025.2463485 |
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