CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies

Background: rheumatoid arthritis (RA) is an autoimmune disease characterized by the occurrence of numerous and complex molecular events that hinder establishing precise biomarkers to study this disease, however, transcriptomic analysis has revealed new pathophysiological elements, suggesting that th...

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Main Authors: Jairo Javier Jattin Balcázar, Daniel Felipe Galeano Sánchez, Gerardo Quintana López
Format: Article
Language:English
Published: Elsevier 2025-04-01
Series:Heliyon
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405844025014896
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author Jairo Javier Jattin Balcázar
Daniel Felipe Galeano Sánchez
Gerardo Quintana López
author_facet Jairo Javier Jattin Balcázar
Daniel Felipe Galeano Sánchez
Gerardo Quintana López
author_sort Jairo Javier Jattin Balcázar
collection DOAJ
description Background: rheumatoid arthritis (RA) is an autoimmune disease characterized by the occurrence of numerous and complex molecular events that hinder establishing precise biomarkers to study this disease, however, transcriptomic analysis has revealed new pathophysiological elements, suggesting that this approach may allow to supply this deficiency. Objective: to develop a systematic review of RNA sequencing (RNA-seq) studies on RA in search of potential biomarkers. Methods: a literature review for RNA-seq studies on human RA was conducted using Embase, PubMed, Web of Science, Scopus, and Cochrane databases. Rayyan was used to compile results and the top 5 up/down-regulated differentially expressed genes (DEGs) were discriminated. Results: 7496 articles were obtained across the databases, selecting 79 papers involving 2423 individuals. DEGs were determined to be at least 38125, but 255 and 124 up- and down-regulated DEGs were detected after filtering by the top 5. Most biomarkers described were related to disease progression (61 %), while the most common analysis and tissue studied were principal component analysis (52 %) and blood (39 %), respectively. The expression of CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF was found to be modified after therapeutic intervention, suggesting that these are indicators of disease activity and potential therapeutic failure and thus the molecules could constitute potential biomarkers in RA. Conclusion: biomarker research via RNA-seq may allow underpinning molecular events to further understand disease progression and the impact of treatment.
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spelling doaj-art-09259a832acc4ebebbc15ed66078aa9f2025-08-20T02:20:00ZengElsevierHeliyon2405-84402025-04-01119e4310810.1016/j.heliyon.2025.e43108CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studiesJairo Javier Jattin Balcázar0Daniel Felipe Galeano Sánchez1Gerardo Quintana López2Universidad Nacional de Colombia–Sede Bogotá, Facultad de Medicina, Grupo Reumavance, Carrera 45#26-85, Colombia; Universidad Nacional de Colombia–Sede Bogotá, Facultad de Ingeniería, Grupo LISI, Carrera 45#26-85, Colombia; Corresponding author. Universidad Nacional de Colombia–Sede Bogotá, Facultad de Medicina, Grupo Reumavance, Carrera 45#26-85, Colombia.Universidad Nacional de Colombia–Sede Bogotá, Facultad de Medicina, Grupo Reumavance, Carrera 45#26-85, ColombiaUniversidad Nacional de Colombia–Sede Bogotá, Facultad de Medicina, Grupo Reumavance, Carrera 45#26-85, ColombiaBackground: rheumatoid arthritis (RA) is an autoimmune disease characterized by the occurrence of numerous and complex molecular events that hinder establishing precise biomarkers to study this disease, however, transcriptomic analysis has revealed new pathophysiological elements, suggesting that this approach may allow to supply this deficiency. Objective: to develop a systematic review of RNA sequencing (RNA-seq) studies on RA in search of potential biomarkers. Methods: a literature review for RNA-seq studies on human RA was conducted using Embase, PubMed, Web of Science, Scopus, and Cochrane databases. Rayyan was used to compile results and the top 5 up/down-regulated differentially expressed genes (DEGs) were discriminated. Results: 7496 articles were obtained across the databases, selecting 79 papers involving 2423 individuals. DEGs were determined to be at least 38125, but 255 and 124 up- and down-regulated DEGs were detected after filtering by the top 5. Most biomarkers described were related to disease progression (61 %), while the most common analysis and tissue studied were principal component analysis (52 %) and blood (39 %), respectively. The expression of CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF was found to be modified after therapeutic intervention, suggesting that these are indicators of disease activity and potential therapeutic failure and thus the molecules could constitute potential biomarkers in RA. Conclusion: biomarker research via RNA-seq may allow underpinning molecular events to further understand disease progression and the impact of treatment.http://www.sciencedirect.com/science/article/pii/S2405844025014896Rheumatoid arthritisRNA-SeqTranscriptomeBiomarkers
spellingShingle Jairo Javier Jattin Balcázar
Daniel Felipe Galeano Sánchez
Gerardo Quintana López
CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
Heliyon
Rheumatoid arthritis
RNA-Seq
Transcriptome
Biomarkers
title CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
title_full CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
title_fullStr CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
title_full_unstemmed CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
title_short CD20, CTLA4, CXCL9, IL18RAP, IL-6, SOCS2, and TNF as potential biomarkers for rheumatoid arthritis disease progression: Systematic review of RNA-seq studies
title_sort cd20 ctla4 cxcl9 il18rap il 6 socs2 and tnf as potential biomarkers for rheumatoid arthritis disease progression systematic review of rna seq studies
topic Rheumatoid arthritis
RNA-Seq
Transcriptome
Biomarkers
url http://www.sciencedirect.com/science/article/pii/S2405844025014896
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