A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity

Implantable telemetry biosensors have become powerful tools for continuous physiological monitoring with minimal animal perturbation. However, commercially available implants are relatively oversized for small animals such as mice and have limited transmission range, leading to concerns about animal...

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Main Authors: Wendi Shi, Hao Huang, Xueting Sun, Qihui Jia, Yu Zhou, Maohua Zhu, Mingqiang Tian, Zhuofan Li, Zepeng Zhang, Tongfei A. Wang, Lei Zhang
Format: Article
Language:English
Published: MDPI AG 2025-06-01
Series:Bioengineering
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Online Access:https://www.mdpi.com/2306-5354/12/6/673
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author Wendi Shi
Hao Huang
Xueting Sun
Qihui Jia
Yu Zhou
Maohua Zhu
Mingqiang Tian
Zhuofan Li
Zepeng Zhang
Tongfei A. Wang
Lei Zhang
author_facet Wendi Shi
Hao Huang
Xueting Sun
Qihui Jia
Yu Zhou
Maohua Zhu
Mingqiang Tian
Zhuofan Li
Zepeng Zhang
Tongfei A. Wang
Lei Zhang
author_sort Wendi Shi
collection DOAJ
description Implantable telemetry biosensors have become powerful tools for continuous physiological monitoring with minimal animal perturbation. However, commercially available implants are relatively oversized for small animals such as mice and have limited transmission range, leading to concerns about animal welfare, experiment scenarios, and the reliability of the data. In this study, we designed a telemetry system that tracks the animals’ body temperature and locomotor activity in real time. The implant integrates a temperature sensor with a 3-axis accelerometer and is capable of wirelessly transmitting data over a 40 m mesh network. The implant’s temperature performance was evaluated in bench tests, showing a response rate of 0.2 °C/s, drift ≤ 0.03 °C within 31 days, and a standard deviation of 0.035 °C across three identically designed implants. Meanwhile, the in vivo implant’s locomotion recordings showed strong agreement with computer vision analysis with a correlation coefficient of r = 0.95 (<i>p</i> < 0.001), and their body temperature recordings were aligned to differential states of rest, exercise, or post-exercise recovery. The results demonstrate stable and highly accurate performance over the 30-day implantation period. Its ability to minimize behavioral interference while enabling long-term continuous monitoring highlights its value in both biomedical and animal behavior research.
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spelling doaj-art-09076a3f2b2e45cf92e8a64bee459ece2025-08-20T03:27:13ZengMDPI AGBioengineering2306-53542025-06-0112667310.3390/bioengineering12060673A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor ActivityWendi Shi0Hao Huang1Xueting Sun2Qihui Jia3Yu Zhou4Maohua Zhu5Mingqiang Tian6Zhuofan Li7Zepeng Zhang8Tongfei A. Wang9Lei Zhang10Beijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaNeuCyber NeuroTech (Beijing) Co., Ltd., Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaBeijing Institute for Brain Research, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 102206, ChinaImplantable telemetry biosensors have become powerful tools for continuous physiological monitoring with minimal animal perturbation. However, commercially available implants are relatively oversized for small animals such as mice and have limited transmission range, leading to concerns about animal welfare, experiment scenarios, and the reliability of the data. In this study, we designed a telemetry system that tracks the animals’ body temperature and locomotor activity in real time. The implant integrates a temperature sensor with a 3-axis accelerometer and is capable of wirelessly transmitting data over a 40 m mesh network. The implant’s temperature performance was evaluated in bench tests, showing a response rate of 0.2 °C/s, drift ≤ 0.03 °C within 31 days, and a standard deviation of 0.035 °C across three identically designed implants. Meanwhile, the in vivo implant’s locomotion recordings showed strong agreement with computer vision analysis with a correlation coefficient of r = 0.95 (<i>p</i> < 0.001), and their body temperature recordings were aligned to differential states of rest, exercise, or post-exercise recovery. The results demonstrate stable and highly accurate performance over the 30-day implantation period. Its ability to minimize behavioral interference while enabling long-term continuous monitoring highlights its value in both biomedical and animal behavior research.https://www.mdpi.com/2306-5354/12/6/673telemetry systemimplantable biosensorsphysiological monitoring
spellingShingle Wendi Shi
Hao Huang
Xueting Sun
Qihui Jia
Yu Zhou
Maohua Zhu
Mingqiang Tian
Zhuofan Li
Zepeng Zhang
Tongfei A. Wang
Lei Zhang
A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
Bioengineering
telemetry system
implantable biosensors
physiological monitoring
title A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
title_full A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
title_fullStr A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
title_full_unstemmed A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
title_short A Miniaturized Implantable Telemetry Biosensor for the Long-Term Dual-Modality Monitoring of Core Temperature and Locomotor Activity
title_sort miniaturized implantable telemetry biosensor for the long term dual modality monitoring of core temperature and locomotor activity
topic telemetry system
implantable biosensors
physiological monitoring
url https://www.mdpi.com/2306-5354/12/6/673
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