Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions

Macrophomina phaseolina (Tassi) Goid. is a fungal pathogen causes charcoal rot disease (Sin: Rhizoctonia bataticola) and is responsible for significant yield losses in many plants. In our study, we aimed to evaluate the antagonistic ability of 39 different bacteria, isolated from the fields of sugar...

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Main Authors: Özden Salman, Fatma Rana Bayram, Nuh Boyraz, Raziye Koçak
Format: Article
Language:English
Published: Hasan Eleroğlu 2021-12-01
Series:Turkish Journal of Agriculture: Food Science and Technology
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Online Access:http://www.agrifoodscience.com/index.php/TURJAF/article/view/4882
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author Özden Salman
Fatma Rana Bayram
Nuh Boyraz
Raziye Koçak
author_facet Özden Salman
Fatma Rana Bayram
Nuh Boyraz
Raziye Koçak
author_sort Özden Salman
collection DOAJ
description Macrophomina phaseolina (Tassi) Goid. is a fungal pathogen causes charcoal rot disease (Sin: Rhizoctonia bataticola) and is responsible for significant yield losses in many plants. In our study, we aimed to evaluate the antagonistic ability of 39 different bacteria, isolated from the fields of sugar beet in 2019, against the pathogen Macrophomina phaseolina isolated from sugar beet, beans and chickpeas. Approximately 31% of the bacteria showed antibiosis effect against the pathogen. It was determined that the effectiveness level of Lelliottia amnigena, Bacillus atrophaeus, B.pumilus and B. cereus (7 isolates) was moderate to high against Macrophomina phaseolina. Bacillus atrophaeus (PTo15-1a) showed the highest efficacy of 80%, 72.94% and 82.35% against Macrophomina phaseolina of chickpea, bean and sugar beet respectively. Lelliottia amnigena (Pto 14-1b) was moderately effective (57.78%) against the chickpea isolate of the pathogen. It was observed that of the seven Bacillus cereus isolates used in the experiment, three isolates (Pto14-1a, Pto12-1b, Pto17-1b) were highly effective against the chickpea pathogen, two (Pto12-1b, Pto14-2b) against bean pathogen, and one (Pto15-1b) against sugar beet isolate. Results have shown varied level of antagonism by different test bacterial against different Macrophomina phaseolina isolates, while the highest level of antibiosis shown by Bacillus atrophaeus against all pathogenic isolates indicated that it can be a potential future bioagent in managing the disease.
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spelling doaj-art-0907332949e04c5fa6d0f66cd41be8742025-08-20T03:55:29ZengHasan EleroğluTurkish Journal of Agriculture: Food Science and Technology2148-127X2021-12-019sp2474247910.24925/turjaf.v9isp.2474-2479.48822318Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro ConditionsÖzden Salman0Fatma Rana Bayram1Nuh Boyraz2Raziye Koçak3Department of Plant Protection, Faculty of Agriculture, Selçuk University, 42250 KonyaSafa Agriculture Joint Stock Company, 42250 KonyaDepartment of Plant Protection, Faculty of Agriculture, Selçuk University, 42250 KonyaAnimal and Plant Production Department, Çumra Vocational School, Selçuk University, 42500 KonyaMacrophomina phaseolina (Tassi) Goid. is a fungal pathogen causes charcoal rot disease (Sin: Rhizoctonia bataticola) and is responsible for significant yield losses in many plants. In our study, we aimed to evaluate the antagonistic ability of 39 different bacteria, isolated from the fields of sugar beet in 2019, against the pathogen Macrophomina phaseolina isolated from sugar beet, beans and chickpeas. Approximately 31% of the bacteria showed antibiosis effect against the pathogen. It was determined that the effectiveness level of Lelliottia amnigena, Bacillus atrophaeus, B.pumilus and B. cereus (7 isolates) was moderate to high against Macrophomina phaseolina. Bacillus atrophaeus (PTo15-1a) showed the highest efficacy of 80%, 72.94% and 82.35% against Macrophomina phaseolina of chickpea, bean and sugar beet respectively. Lelliottia amnigena (Pto 14-1b) was moderately effective (57.78%) against the chickpea isolate of the pathogen. It was observed that of the seven Bacillus cereus isolates used in the experiment, three isolates (Pto14-1a, Pto12-1b, Pto17-1b) were highly effective against the chickpea pathogen, two (Pto12-1b, Pto14-2b) against bean pathogen, and one (Pto15-1b) against sugar beet isolate. Results have shown varied level of antagonism by different test bacterial against different Macrophomina phaseolina isolates, while the highest level of antibiosis shown by Bacillus atrophaeus against all pathogenic isolates indicated that it can be a potential future bioagent in managing the disease.http://www.agrifoodscience.com/index.php/TURJAF/article/view/4882macrophominaantibiosisbacillusbioagentcharcoal rot
spellingShingle Özden Salman
Fatma Rana Bayram
Nuh Boyraz
Raziye Koçak
Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
Turkish Journal of Agriculture: Food Science and Technology
macrophomina
antibiosis
bacillus
bioagent
charcoal rot
title Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
title_full Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
title_fullStr Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
title_full_unstemmed Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
title_short Determination of the Antagonistic Effects of Some Rhizospheric Bacteria against Macrophomina phaseolina under In Vitro Conditions
title_sort determination of the antagonistic effects of some rhizospheric bacteria against macrophomina phaseolina under in vitro conditions
topic macrophomina
antibiosis
bacillus
bioagent
charcoal rot
url http://www.agrifoodscience.com/index.php/TURJAF/article/view/4882
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AT fatmaranabayram determinationoftheantagonisticeffectsofsomerhizosphericbacteriaagainstmacrophominaphaseolinaunderinvitroconditions
AT nuhboyraz determinationoftheantagonisticeffectsofsomerhizosphericbacteriaagainstmacrophominaphaseolinaunderinvitroconditions
AT raziyekocak determinationoftheantagonisticeffectsofsomerhizosphericbacteriaagainstmacrophominaphaseolinaunderinvitroconditions