The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue
Cerebral palsy is the most common cause of disability in children worldwide, estimated prevalence of 1.5–4 per 1000 children; the higher prevalence in low-resource populations (up to 10 per 1000 children). Brain-derived neurotrophic factor (BDNF) is potent modulator of many neuronal functions that...
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University of Baghdad, College of Science for Women
2024-12-01
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| Series: | مجلة بغداد للعلوم |
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| Online Access: | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9588 |
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| author | Dudy Aldiansyah Sarma Nursani Lumbanraja Khairul Putra Surbakti Isti Ilmiati Fujiati Agus Sulistyono |
| author_facet | Dudy Aldiansyah Sarma Nursani Lumbanraja Khairul Putra Surbakti Isti Ilmiati Fujiati Agus Sulistyono |
| author_sort | Dudy Aldiansyah |
| collection | DOAJ |
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Cerebral palsy is the most common cause of disability in children worldwide, estimated prevalence of 1.5–4 per 1000 children; the higher prevalence in low-resource populations (up to 10 per 1000 children). Brain-derived neurotrophic factor (BDNF) is potent modulator of many neuronal functions that protect the newborn or developing brain from ischemic injury. The expression of GluN3A, which plays a neuroprotective role, is rapidly induced during cerebral ischemia and hypoxia. This study assessed the effect of piracetam administration on BDNF and GluN3A levels in the brain tissue to determine its potential to prevent cerebral palsy. In this experimental study with a post-test-only control group design, a rat model of cerebral palsy was established by injecting pregnant rats with LPS on gestation days 15, 17, and 19; piracetam was administered orally on day 10.5. BDNF and GluN3A protein levels and mRNA expression in the foetal brain tissue of 36 subjects were evaluated with enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR). BDNF and GluN3A protein levels in the foetal brain differed significantly between the control and treatment groups (p < 0.05). A decrease in the mRNA and protein levels of BDNF and GluN3A was observed in all treatment groups, but the statistical analysis of RT-PCR did not reveal significant differences between the control and treatment groups (p > 0.05). These results indicate that piracetam can prevent cerebral palsy in a foetal rat model established via prenatal LPS injection, as assessed by the protein expression of BDNF and GluN3A mRNA.
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| format | Article |
| id | doaj-art-090140c208db4edeb166897c5ebca290 |
| institution | DOAJ |
| issn | 2078-8665 2411-7986 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | University of Baghdad, College of Science for Women |
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| series | مجلة بغداد للعلوم |
| spelling | doaj-art-090140c208db4edeb166897c5ebca2902025-08-20T03:15:51ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862024-12-012112(Suppl.)10.21123/bsj.2024.9588The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain TissueDudy Aldiansyah0https://orcid.org/0000-0001-9601-2855Sarma Nursani Lumbanraja1Khairul Putra Surbakti 2Isti Ilmiati Fujiati3Agus Sulistyono4Philosophy Doctor in Medicine Program, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. and Department of Obstetrics and Gynaecology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia./Department of Obstetrics and Gynaecology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia.Department of Obstetrics and Gynaecology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia.Departement of Neurology, Medical Faculty, Universitas Sumatera Utara, Medan, Indonesia.Department of Community Medicine, Public Health, Faculty of Medicine, Universitas Sumatera Utara, Medan Indonesia.Department Obstetrics & Gynecology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia. Cerebral palsy is the most common cause of disability in children worldwide, estimated prevalence of 1.5–4 per 1000 children; the higher prevalence in low-resource populations (up to 10 per 1000 children). Brain-derived neurotrophic factor (BDNF) is potent modulator of many neuronal functions that protect the newborn or developing brain from ischemic injury. The expression of GluN3A, which plays a neuroprotective role, is rapidly induced during cerebral ischemia and hypoxia. This study assessed the effect of piracetam administration on BDNF and GluN3A levels in the brain tissue to determine its potential to prevent cerebral palsy. In this experimental study with a post-test-only control group design, a rat model of cerebral palsy was established by injecting pregnant rats with LPS on gestation days 15, 17, and 19; piracetam was administered orally on day 10.5. BDNF and GluN3A protein levels and mRNA expression in the foetal brain tissue of 36 subjects were evaluated with enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR). BDNF and GluN3A protein levels in the foetal brain differed significantly between the control and treatment groups (p < 0.05). A decrease in the mRNA and protein levels of BDNF and GluN3A was observed in all treatment groups, but the statistical analysis of RT-PCR did not reveal significant differences between the control and treatment groups (p > 0.05). These results indicate that piracetam can prevent cerebral palsy in a foetal rat model established via prenatal LPS injection, as assessed by the protein expression of BDNF and GluN3A mRNA. https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9588BDNF, Cerebral palsy, GluN3A, piracetam, rats. |
| spellingShingle | Dudy Aldiansyah Sarma Nursani Lumbanraja Khairul Putra Surbakti Isti Ilmiati Fujiati Agus Sulistyono The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue مجلة بغداد للعلوم BDNF, Cerebral palsy, GluN3A, piracetam, rats. |
| title | The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue |
| title_full | The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue |
| title_fullStr | The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue |
| title_full_unstemmed | The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue |
| title_short | The Effect of Piracetam Administration on Cerebral Palsy Prevention in Rat Fetuses Born To Pregnant Rats by Determining Bdnf Levels in Brain Tissue |
| title_sort | effect of piracetam administration on cerebral palsy prevention in rat fetuses born to pregnant rats by determining bdnf levels in brain tissue |
| topic | BDNF, Cerebral palsy, GluN3A, piracetam, rats. |
| url | https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9588 |
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