<i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation
<i>Patrinia scabiosaefolia</i> L. (<i>P. scabiosaefolia</i>), a traditional food and medicinal plant, is used to treat internal inflammation. This study investigated the mechanisms by which <i>P. scabiosaefolia</i> improves ulcerative colitis (UC) via combined UHP...
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| Format: | Article |
| Language: | English |
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MDPI AG
2025-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/14/7/1145 |
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| author | Longfei Zhang Xiaoxiao Liu Mingze Xu Xinyi Cheng Ning Li Haiyan Xu Yining Feng Tianzhu Guan Lixia Xiao |
| author_facet | Longfei Zhang Xiaoxiao Liu Mingze Xu Xinyi Cheng Ning Li Haiyan Xu Yining Feng Tianzhu Guan Lixia Xiao |
| author_sort | Longfei Zhang |
| collection | DOAJ |
| description | <i>Patrinia scabiosaefolia</i> L. (<i>P. scabiosaefolia</i>), a traditional food and medicinal plant, is used to treat internal inflammation. This study investigated the mechanisms by which <i>P. scabiosaefolia</i> improves ulcerative colitis (UC) via combined UHPLC-OE-MS/MS, network pharmacology, molecular docking, and animal experiments. A total of 72 compounds were detected in the <i>P. scabiosaefolia</i> extraction, with 15 key components (ranking by degree value) selected for further analysis. GO enrichment analysis suggested that PS may alleviate UC-related renal dysfunction by modulating immune responses, inflammation, and cell signaling pathways. Based on protein–protein interaction results, five core targets of <i>P. scabiosaefolia</i> in UC (ranking by degree value) were identified, and molecular docking revealed strong binding free affinity (<−7 kcal/mol) of active components (Vulgarin and 4-(Diphenylphosphino)benzoic acid) with TNF, AKT1, CASP3, BCL2, and MMP9. In animal experiments, <i>P. scabiosaefolia</i>-treated mice showed significant reductions in IL-6, TNF-α, LPS, and D-Lactate levels (<i>p</i> < 0.05); improved colon histopathological damage; and significantly increased the mRNA expression of tight junction proteins (ZO-1, Claudin, OCC) in colon tissue (<i>p</i> < 0.05). Furthermore, <i>P. scabiosaefolia</i>-treated mice exhibited a significant increase in beneficial gut bacteria (<i>Enterococcus</i> and <i>Lactobacillus</i>) (<i>p</i> < 0.05), effectively restoring the gut imbalance caused by DSS. In conclusion, <i>P. scabiosaefolia</i> can treat UC through the modulation of the intestinal microecology. |
| format | Article |
| id | doaj-art-08f2dd15bfdf4cf1a4d1effe513025d0 |
| institution | OA Journals |
| issn | 2304-8158 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-08f2dd15bfdf4cf1a4d1effe513025d02025-08-20T02:09:13ZengMDPI AGFoods2304-81582025-03-01147114510.3390/foods14071145<i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental ValidationLongfei Zhang0Xiaoxiao Liu1Mingze Xu2Xinyi Cheng3Ning Li4Haiyan Xu5Yining Feng6Tianzhu Guan7Lixia Xiao8College of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, ChinaCollege of Food Science and Technology, Yangzhou University, Yangzhou 225000, China<i>Patrinia scabiosaefolia</i> L. (<i>P. scabiosaefolia</i>), a traditional food and medicinal plant, is used to treat internal inflammation. This study investigated the mechanisms by which <i>P. scabiosaefolia</i> improves ulcerative colitis (UC) via combined UHPLC-OE-MS/MS, network pharmacology, molecular docking, and animal experiments. A total of 72 compounds were detected in the <i>P. scabiosaefolia</i> extraction, with 15 key components (ranking by degree value) selected for further analysis. GO enrichment analysis suggested that PS may alleviate UC-related renal dysfunction by modulating immune responses, inflammation, and cell signaling pathways. Based on protein–protein interaction results, five core targets of <i>P. scabiosaefolia</i> in UC (ranking by degree value) were identified, and molecular docking revealed strong binding free affinity (<−7 kcal/mol) of active components (Vulgarin and 4-(Diphenylphosphino)benzoic acid) with TNF, AKT1, CASP3, BCL2, and MMP9. In animal experiments, <i>P. scabiosaefolia</i>-treated mice showed significant reductions in IL-6, TNF-α, LPS, and D-Lactate levels (<i>p</i> < 0.05); improved colon histopathological damage; and significantly increased the mRNA expression of tight junction proteins (ZO-1, Claudin, OCC) in colon tissue (<i>p</i> < 0.05). Furthermore, <i>P. scabiosaefolia</i>-treated mice exhibited a significant increase in beneficial gut bacteria (<i>Enterococcus</i> and <i>Lactobacillus</i>) (<i>p</i> < 0.05), effectively restoring the gut imbalance caused by DSS. In conclusion, <i>P. scabiosaefolia</i> can treat UC through the modulation of the intestinal microecology.https://www.mdpi.com/2304-8158/14/7/1145<i>Patrinia scabiosaefolia</i>ulcerative colitisUHPLC-OE-MS/MSintestinal microecology |
| spellingShingle | Longfei Zhang Xiaoxiao Liu Mingze Xu Xinyi Cheng Ning Li Haiyan Xu Yining Feng Tianzhu Guan Lixia Xiao <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation Foods <i>Patrinia scabiosaefolia</i> ulcerative colitis UHPLC-OE-MS/MS intestinal microecology |
| title | <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation |
| title_full | <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation |
| title_fullStr | <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation |
| title_full_unstemmed | <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation |
| title_short | <i>Patrinia scabiosaefolia</i> L. Modulates the Intestinal Microecology to Treat DSS-Induced Ulcerative Colitis: UHPLC-OE-MS/MS, Network Pharmacology, and Experimental Validation |
| title_sort | i patrinia scabiosaefolia i l modulates the intestinal microecology to treat dss induced ulcerative colitis uhplc oe ms ms network pharmacology and experimental validation |
| topic | <i>Patrinia scabiosaefolia</i> ulcerative colitis UHPLC-OE-MS/MS intestinal microecology |
| url | https://www.mdpi.com/2304-8158/14/7/1145 |
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