Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats
Background/Aims. Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. Methods. Liver cirrhotic r...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2018/5309286 |
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| author | Changjun Jia Chaoliu Dai Hailiang Wang Yi Wan Yunyu Qiao Feng Xu Songlin Peng Yang Zhao Chuang Zhao Liang Zhao |
| author_facet | Changjun Jia Chaoliu Dai Hailiang Wang Yi Wan Yunyu Qiao Feng Xu Songlin Peng Yang Zhao Chuang Zhao Liang Zhao |
| author_sort | Changjun Jia |
| collection | DOAJ |
| description | Background/Aims. Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. Methods. Liver cirrhotic rats had undertaken Pringle maneuver (PR), hemihepatic vascular occlusion (HH), or hepatic blood inflow occlusion without hemihepatic artery control (WH). Levels of tumor necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-κB), toll-like receptor 4 (TLR4), TIR-domain-containing adapter-inducing interferon-β (TRIF), and hemeoxygenase 1 (HMOX1) were assayed. Results. The histopathologic analysis displayed that liver harm was more prominent in the PR group, but similar in the HH and WH groups. The HH and WH groups responded to hepatic I/R inflammation similarly but better than the PR group. Mechanical studies suggested that TNF-α/NF-κB signaling and TLR4/TRIF transduction pathways were associated with the differential effects. In addition, the HH and WH groups had significantly higher levels of hepatic HMOX1 (P<0.05) than the PR group. Conclusions. HH and WH confer better preservation of liver function and protection than the Pringle maneuver in combating I/R injury. Upregulation of HMOX1 may lead to better protection and clinical outcomes after liver resection. |
| format | Article |
| id | doaj-art-08e954e122de4baab820e8ea9094cd61 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
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| series | Gastroenterology Research and Practice |
| spelling | doaj-art-08e954e122de4baab820e8ea9094cd612025-08-20T03:55:33ZengWileyGastroenterology Research and Practice1687-61211687-630X2018-01-01201810.1155/2018/53092865309286Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in RatsChangjun Jia0Chaoliu Dai1Hailiang Wang2Yi Wan3Yunyu Qiao4Feng Xu5Songlin Peng6Yang Zhao7Chuang Zhao8Liang Zhao9Department of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaDepartment of Hepatobiliary and Splenic Surgery, Shengjing Hospital of China Medical University, Shenyang, Liaoning 110004, ChinaBackground/Aims. Hepatic ischemia-reperfusion (I/R) injury is a serious concern during hepatic vascular occlusion. The objectives of this study were to assess effects of three techniques for hepatic vascular occlusion on I/R injury and to explore the underlying mechanisms. Methods. Liver cirrhotic rats had undertaken Pringle maneuver (PR), hemihepatic vascular occlusion (HH), or hepatic blood inflow occlusion without hemihepatic artery control (WH). Levels of tumor necrosis factor alpha (TNF-α), nuclear factor kappa B (NF-κB), toll-like receptor 4 (TLR4), TIR-domain-containing adapter-inducing interferon-β (TRIF), and hemeoxygenase 1 (HMOX1) were assayed. Results. The histopathologic analysis displayed that liver harm was more prominent in the PR group, but similar in the HH and WH groups. The HH and WH groups responded to hepatic I/R inflammation similarly but better than the PR group. Mechanical studies suggested that TNF-α/NF-κB signaling and TLR4/TRIF transduction pathways were associated with the differential effects. In addition, the HH and WH groups had significantly higher levels of hepatic HMOX1 (P<0.05) than the PR group. Conclusions. HH and WH confer better preservation of liver function and protection than the Pringle maneuver in combating I/R injury. Upregulation of HMOX1 may lead to better protection and clinical outcomes after liver resection.http://dx.doi.org/10.1155/2018/5309286 |
| spellingShingle | Changjun Jia Chaoliu Dai Hailiang Wang Yi Wan Yunyu Qiao Feng Xu Songlin Peng Yang Zhao Chuang Zhao Liang Zhao Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats Gastroenterology Research and Practice |
| title | Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats |
| title_full | Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats |
| title_fullStr | Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats |
| title_full_unstemmed | Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats |
| title_short | Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats |
| title_sort | differential effects of three techniques for hepatic vascular exclusion during resection for liver cirrhosis on hepatic ischemia reperfusion injury in rats |
| url | http://dx.doi.org/10.1155/2018/5309286 |
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