CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp
Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. Given the obvious cytopathic effect (CPE) in the pres...
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KeAi Communications Co. Ltd.
2025-01-01
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| Series: | Water Biology and Security |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772735124000957 |
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| author | Jie Zhang Liqiang Zhang Xiaoman Wu Minhui Tao Yang Chen Mingxian Chang |
| author_facet | Jie Zhang Liqiang Zhang Xiaoman Wu Minhui Tao Yang Chen Mingxian Chang |
| author_sort | Jie Zhang |
| collection | DOAJ |
| description | Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. Given the obvious cytopathic effect (CPE) in the present available cell lines is only caused by GCRV-I, but GCRV-II is the current popular and fatal strain in grass carp, GCRV-I and GCRV-II are used in cell lines and in grass carp, respectively. In vitro studies in CIK cells revealed that gcPI4KB interacted with NS80 and VP3 of GCRV-I, and that gcPI4KB was recruited by NS80 for promoting the generation of GCRV viral inclusion bodies (VIBs). Since the negative regulatory role of gcPI4KB in GCRV infection was confirmed by in vitro data, we performed gene editing of gcPI4KB in grass carp. We found that PI4KB F0 juvenile grass carp crispants have obvious advantages in promoting growth and in resisting GCRV-II infection. Compared with uninfected WT grass carp, the uninfected PI4KB F0 juvenile grass carp crispants exhibit a higher expression level of many genes involved in growth- and development-related metabolic pathways such as the FoxO signaling pathway and insulin signaling pathway. Compared with WT grass carp without infection, PI4KB F0 juvenile grass carp crispants without infection or WT grass carp infected with GCRV-II, higher expression levels for many genes involved in metabolic diseases and viral infections were observed in the liver from PI4KB F0 juvenile grass carp crispants infected with GCRV-II. Altogether, the present study suggests the mechanism of gcPI4KB in facilitating GCRV replication, the signaling pathways regulated by gcPI4KB, and the possibility to obtain grass carp resistant to hemorrhagic disease via gene editing of PI4KB. |
| format | Article |
| id | doaj-art-08dd59d316be47ce8f28e3c7c114cc04 |
| institution | Kabale University |
| issn | 2772-7351 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | KeAi Communications Co. Ltd. |
| record_format | Article |
| series | Water Biology and Security |
| spelling | doaj-art-08dd59d316be47ce8f28e3c7c114cc042025-01-27T04:22:42ZengKeAi Communications Co. Ltd.Water Biology and Security2772-73512025-01-0141100323CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carpJie Zhang0Liqiang Zhang1Xiaoman Wu2Minhui Tao3Yang Chen4Mingxian Chang5Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, ChinaWuhan Academy of Agricultural Sciences, Wuhan, 430072, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, ChinaKey Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China; College of Advanced Agricultural Sciences, University of Chinese Academy of Sciences, Beijing, 100049, China; Corresponding author. Key Laboratory of Breeding Biotechnology and Sustainable Aquaculture (CAS), State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, Wuhan, 430072, China.Few studies have reported obtaining grass carp resistant to hemorrhagic disease via gene editing in commercial fish. Here, we demonstrate that the expression and activity of grass carp PI4KB (gcPI4KB) are vital for GCRV-I and GCRV-II replication. Given the obvious cytopathic effect (CPE) in the present available cell lines is only caused by GCRV-I, but GCRV-II is the current popular and fatal strain in grass carp, GCRV-I and GCRV-II are used in cell lines and in grass carp, respectively. In vitro studies in CIK cells revealed that gcPI4KB interacted with NS80 and VP3 of GCRV-I, and that gcPI4KB was recruited by NS80 for promoting the generation of GCRV viral inclusion bodies (VIBs). Since the negative regulatory role of gcPI4KB in GCRV infection was confirmed by in vitro data, we performed gene editing of gcPI4KB in grass carp. We found that PI4KB F0 juvenile grass carp crispants have obvious advantages in promoting growth and in resisting GCRV-II infection. Compared with uninfected WT grass carp, the uninfected PI4KB F0 juvenile grass carp crispants exhibit a higher expression level of many genes involved in growth- and development-related metabolic pathways such as the FoxO signaling pathway and insulin signaling pathway. Compared with WT grass carp without infection, PI4KB F0 juvenile grass carp crispants without infection or WT grass carp infected with GCRV-II, higher expression levels for many genes involved in metabolic diseases and viral infections were observed in the liver from PI4KB F0 juvenile grass carp crispants infected with GCRV-II. Altogether, the present study suggests the mechanism of gcPI4KB in facilitating GCRV replication, the signaling pathways regulated by gcPI4KB, and the possibility to obtain grass carp resistant to hemorrhagic disease via gene editing of PI4KB.http://www.sciencedirect.com/science/article/pii/S2772735124000957PI4KBGCRVViral inclusion bodiesPI4KB F0 juvenile grass carp crispantsCRISPR/Cas9 |
| spellingShingle | Jie Zhang Liqiang Zhang Xiaoman Wu Minhui Tao Yang Chen Mingxian Chang CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp Water Biology and Security PI4KB GCRV Viral inclusion bodies PI4KB F0 juvenile grass carp crispants CRISPR/Cas9 |
| title | CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp |
| title_full | CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp |
| title_fullStr | CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp |
| title_full_unstemmed | CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp |
| title_short | CRISPR/Cas9 mutagenesis reveals an essential role of PI4KB in promoting growth and resisting hemorrhagic disease caused by GCRV-II infection in juvenile grass carp |
| title_sort | crispr cas9 mutagenesis reveals an essential role of pi4kb in promoting growth and resisting hemorrhagic disease caused by gcrv ii infection in juvenile grass carp |
| topic | PI4KB GCRV Viral inclusion bodies PI4KB F0 juvenile grass carp crispants CRISPR/Cas9 |
| url | http://www.sciencedirect.com/science/article/pii/S2772735124000957 |
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