The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer
Despite advances in cancer treatment, breast cancer (BC) remains one of the most common cancers affecting women worldwide. This study aimed to determine serum circFBXW7, circABCB10, and circ0103552 levels and compare BC patients and healthy controls to investigate their roles in the molecular mechan...
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MDPI AG
2024-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/12/862 |
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| author | Burak İlhan Şenol Ender Berkay Kılıç Muhammed Üçüncü Murat Serilmez Ceren Tilgen Yasasever Hilal Oğuz Soydinç Sibel Kuras Bekir Erdoğan Hani Alsaadoni Hasan Karanlık Süleyman Bademler |
| author_facet | Burak İlhan Şenol Ender Berkay Kılıç Muhammed Üçüncü Murat Serilmez Ceren Tilgen Yasasever Hilal Oğuz Soydinç Sibel Kuras Bekir Erdoğan Hani Alsaadoni Hasan Karanlık Süleyman Bademler |
| author_sort | Burak İlhan |
| collection | DOAJ |
| description | Despite advances in cancer treatment, breast cancer (BC) remains one of the most common cancers affecting women worldwide. This study aimed to determine serum circFBXW7, circABCB10, and circ0103552 levels and compare BC patients and healthy controls to investigate their roles in the molecular mechanism of BC and the significance of these circRNAs in BC diagnosis. The study group consisted of 92 patients with BC and 31 healthy controls. Total RNA was isolated from serum samples. Following total RNA, complementary DNA was synthesized from this material. Following complementary DNA analysis, the circRNA levels were analyzed by the qRT-PCR method. Expression levels were evaluated in ΔCt values. High ΔCt values of circFBXW7 and circ0103552 and low ΔCt values of circABCB10 were correlated with BC diagnosis (circFBXW7, <i>p</i> = 0.043, r = 0.183, circ0103552, <i>p</i> < 0.001, r = 0.321, circABCB10, <i>p</i> = 0.001, r = −0.291). According to Fold Change (FC) values, circFBXW7 (FC = 0.30) and circ0103552 (FC = 0.26) showed low expression in the patient group compared to the control group, while circABCB10 (FC = 11.09) showed high expression (<i>p</i> < 0.05, for all comparisons). We think that our study is one of the rare studies investigating the relationship between BC and serum circRNA levels. This study concludes that the significant downregulation of circFBXW7 and circ0103552 and the upregulation of circABCB10 are directly related to the diagnosis of BC and can be used for diagnosis, but further studies are needed to elucidate the molecular mechanism of the relationship between circRNAs and BC. |
| format | Article |
| id | doaj-art-08d6b43dd894440dabfb15bc124bbb7d |
| institution | OA Journals |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-08d6b43dd894440dabfb15bc124bbb7d2025-08-20T02:00:25ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014612143811439310.3390/cimb46120862The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast CancerBurak İlhan0Şenol Ender1Berkay Kılıç2Muhammed Üçüncü3Murat Serilmez4Ceren Tilgen Yasasever5Hilal Oğuz Soydinç6Sibel Kuras7Bekir Erdoğan8Hani Alsaadoni9Hasan Karanlık10Süleyman Bademler11Department of Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TürkiyeDepartment of Surgery, Istanbul Faculty of Medicine, Istanbul University, Istanbul 34093, TürkiyeDepartment of Surgery, Oncology Institute, Istanbul University, Istanbul 34093, TürkiyeDepartment of Anesthesia, Vocational School of Health Services, Istanbul Gelişim University, Istanbul 34310, TürkiyeDepartment of Basic Oncology, Institute of Oncology, Istanbul University, Istanbul 34093, TürkiyeDepartment of Basic Oncology, Institute of Oncology, Istanbul University, Istanbul 34093, TürkiyeDepartment of Basic Oncology, Institute of Oncology, Istanbul University, Istanbul 34093, TürkiyeDepartment of Medical Biochemistry, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34668, TürkiyeDepartment of Physiology, Hamidiye Faculty of Medicine, University of Health Sciences, Istanbul 34668, TürkiyeDepartment of Medical Biology, International School of Medicine, University of Health Sciences, Istanbul 34668, TürkiyeDepartment of Surgery, Oncology Institute, Istanbul University, Istanbul 34093, TürkiyeDepartment of Surgery, Oncology Institute, Istanbul University, Istanbul 34093, TürkiyeDespite advances in cancer treatment, breast cancer (BC) remains one of the most common cancers affecting women worldwide. This study aimed to determine serum circFBXW7, circABCB10, and circ0103552 levels and compare BC patients and healthy controls to investigate their roles in the molecular mechanism of BC and the significance of these circRNAs in BC diagnosis. The study group consisted of 92 patients with BC and 31 healthy controls. Total RNA was isolated from serum samples. Following total RNA, complementary DNA was synthesized from this material. Following complementary DNA analysis, the circRNA levels were analyzed by the qRT-PCR method. Expression levels were evaluated in ΔCt values. High ΔCt values of circFBXW7 and circ0103552 and low ΔCt values of circABCB10 were correlated with BC diagnosis (circFBXW7, <i>p</i> = 0.043, r = 0.183, circ0103552, <i>p</i> < 0.001, r = 0.321, circABCB10, <i>p</i> = 0.001, r = −0.291). According to Fold Change (FC) values, circFBXW7 (FC = 0.30) and circ0103552 (FC = 0.26) showed low expression in the patient group compared to the control group, while circABCB10 (FC = 11.09) showed high expression (<i>p</i> < 0.05, for all comparisons). We think that our study is one of the rare studies investigating the relationship between BC and serum circRNA levels. This study concludes that the significant downregulation of circFBXW7 and circ0103552 and the upregulation of circABCB10 are directly related to the diagnosis of BC and can be used for diagnosis, but further studies are needed to elucidate the molecular mechanism of the relationship between circRNAs and BC.https://www.mdpi.com/1467-3045/46/12/862circFBXW7circABCB10circ0103552circRNAbreast cancer |
| spellingShingle | Burak İlhan Şenol Ender Berkay Kılıç Muhammed Üçüncü Murat Serilmez Ceren Tilgen Yasasever Hilal Oğuz Soydinç Sibel Kuras Bekir Erdoğan Hani Alsaadoni Hasan Karanlık Süleyman Bademler The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer Current Issues in Molecular Biology circFBXW7 circABCB10 circ0103552 circRNA breast cancer |
| title | The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer |
| title_full | The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer |
| title_fullStr | The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer |
| title_full_unstemmed | The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer |
| title_short | The Diagnostic Value of circFBXW7, circABCB10, and circ0103552 Levels in Breast Cancer |
| title_sort | diagnostic value of circfbxw7 circabcb10 and circ0103552 levels in breast cancer |
| topic | circFBXW7 circABCB10 circ0103552 circRNA breast cancer |
| url | https://www.mdpi.com/1467-3045/46/12/862 |
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