Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells
Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2020/8894449 |
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| author | Yan Xu Tao Zhang Yang Chen Qiang Shi Muzhi Li Tian Qin Jianzhong Hu Hongbin Lu Jun Liu Can Chen |
| author_facet | Yan Xu Tao Zhang Yang Chen Qiang Shi Muzhi Li Tian Qin Jianzhong Hu Hongbin Lu Jun Liu Can Chen |
| author_sort | Yan Xu |
| collection | DOAJ |
| description | Current cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to bridge the gap from rodents to humans. In this study, we reported the isolation and characterization of urine-derived stem cells (USCs) from mature healthy beagle dogs. The isolated cells showed fibroblast-like morphology and had good clonogenicity and proliferation. Meanwhile, these cells positively expressed multiple markers of MSCs (CD29, CD44, CD90, and CD73), but negatively expressed for hematopoietic antigens (CD11b, CD34, and CD45). Additionally, after induction culturing, the isolated cells can be differentiated into osteogenic, adipogenic, chondrogenic, and tenogenic lineages. The successful isolation and verification of USCs from canine were useful for studying cell-based therapies and developing new treatments for musculoskeletal injuries using the preclinical canine model. |
| format | Article |
| id | doaj-art-08c48b760ea0447da9030f415946d0e4 |
| institution | OA Journals |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-08c48b760ea0447da9030f415946d0e42025-08-20T02:03:20ZengWileyStem Cells International1687-966X1687-96782020-01-01202010.1155/2020/88944498894449Isolation and Characterization of Multipotent Canine Urine-Derived Stem CellsYan Xu0Tao Zhang1Yang Chen2Qiang Shi3Muzhi Li4Tian Qin5Jianzhong Hu6Hongbin Lu7Jun Liu8Can Chen9Key Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaDepartment of Limbs (Foot and Hand) Microsurgery, Affiliated Chenzhou No.1 People’s Hospital, Southern Medical University, Chenzhou, 423000, ChinaKey Laboratory of Organ Injury, Aging and Regenerative Medicine of Hunan Province, Changsha, 410008, ChinaCurrent cell-based therapies on musculoskeletal tissue regeneration were mostly determined in rodent models. However, a direct translation of those promising cell-based therapies to humans exists a significant hurdle. For solving this problem, canine has been developed as a new large animal model to bridge the gap from rodents to humans. In this study, we reported the isolation and characterization of urine-derived stem cells (USCs) from mature healthy beagle dogs. The isolated cells showed fibroblast-like morphology and had good clonogenicity and proliferation. Meanwhile, these cells positively expressed multiple markers of MSCs (CD29, CD44, CD90, and CD73), but negatively expressed for hematopoietic antigens (CD11b, CD34, and CD45). Additionally, after induction culturing, the isolated cells can be differentiated into osteogenic, adipogenic, chondrogenic, and tenogenic lineages. The successful isolation and verification of USCs from canine were useful for studying cell-based therapies and developing new treatments for musculoskeletal injuries using the preclinical canine model.http://dx.doi.org/10.1155/2020/8894449 |
| spellingShingle | Yan Xu Tao Zhang Yang Chen Qiang Shi Muzhi Li Tian Qin Jianzhong Hu Hongbin Lu Jun Liu Can Chen Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells Stem Cells International |
| title | Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells |
| title_full | Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells |
| title_fullStr | Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells |
| title_full_unstemmed | Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells |
| title_short | Isolation and Characterization of Multipotent Canine Urine-Derived Stem Cells |
| title_sort | isolation and characterization of multipotent canine urine derived stem cells |
| url | http://dx.doi.org/10.1155/2020/8894449 |
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