Characterization of CoCas9 nuclease from Capnocytophaga ochracea
Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Cap...
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| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-12-01
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| Series: | RNA Biology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/15476286.2023.2256578 |
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| author | A. Vasileva P. Selkova A. Arseniev M. Abramova N. Shcheglova O. Musharova I. Mizgirev T. Artamonova M. Khodorkovskii K. Severinov I. Fedorova |
| author_facet | A. Vasileva P. Selkova A. Arseniev M. Abramova N. Shcheglova O. Musharova I. Mizgirev T. Artamonova M. Khodorkovskii K. Severinov I. Fedorova |
| author_sort | A. Vasileva |
| collection | DOAJ |
| description | Cas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Capnocytophaga ochracea, a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5’-NRRWC-3’ PAM and efficiently cleaves DNA in vitro. Functional characterization of CoCas9 opens ways for genetic engineering of C. ochracea using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications. |
| format | Article |
| id | doaj-art-08bbbcf3e60448a3b5a361c6aeec2e26 |
| institution | DOAJ |
| issn | 1547-6286 1555-8584 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | RNA Biology |
| spelling | doaj-art-08bbbcf3e60448a3b5a361c6aeec2e262025-08-20T03:12:56ZengTaylor & Francis GroupRNA Biology1547-62861555-85842023-12-0120175075910.1080/15476286.2023.2256578Characterization of CoCas9 nuclease from Capnocytophaga ochraceaA. Vasileva0P. Selkova1A. Arseniev2M. Abramova3N. Shcheglova4O. Musharova5I. Mizgirev6T. Artamonova7M. Khodorkovskii8K. Severinov9I. Fedorova10Center of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaComplex of NBICS Technologies, National Research Center “Kurchatov Institute”, Moscow, RussiaLaboratory of Carcinogenesis and Aging, N.N. Petrov National Medical Research Center of Oncology, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaComplex of NBICS Technologies, National Research Center “Kurchatov Institute”, Moscow, RussiaCenter of Nanobiotechnology, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, RussiaCas9 nucleases are widely used for genome editing and engineering. Cas9 enzymes encoded by CRISPR-Cas defence systems of various prokaryotic organisms possess different properties such as target site preferences, size, and DNA cleavage efficiency. Here, we biochemically characterized CoCas9 from Capnocytophaga ochracea, a bacterium that inhabits the oral cavity of humans and contributes to plaque formation on teeth. CoCas9 recognizes a novel 5’-NRRWC-3’ PAM and efficiently cleaves DNA in vitro. Functional characterization of CoCas9 opens ways for genetic engineering of C. ochracea using its endogenous CRISPR-Cas system. The novel PAM requirement makes CoCas9 potentially useful in genome editing applications.https://www.tandfonline.com/doi/10.1080/15476286.2023.2256578CoCas9Cas9Capnocytophaga ochraceaCRISPR-CasRNA-guided nuclease |
| spellingShingle | A. Vasileva P. Selkova A. Arseniev M. Abramova N. Shcheglova O. Musharova I. Mizgirev T. Artamonova M. Khodorkovskii K. Severinov I. Fedorova Characterization of CoCas9 nuclease from Capnocytophaga ochracea RNA Biology CoCas9 Cas9 Capnocytophaga ochracea CRISPR-Cas RNA-guided nuclease |
| title | Characterization of CoCas9 nuclease from Capnocytophaga ochracea |
| title_full | Characterization of CoCas9 nuclease from Capnocytophaga ochracea |
| title_fullStr | Characterization of CoCas9 nuclease from Capnocytophaga ochracea |
| title_full_unstemmed | Characterization of CoCas9 nuclease from Capnocytophaga ochracea |
| title_short | Characterization of CoCas9 nuclease from Capnocytophaga ochracea |
| title_sort | characterization of cocas9 nuclease from capnocytophaga ochracea |
| topic | CoCas9 Cas9 Capnocytophaga ochracea CRISPR-Cas RNA-guided nuclease |
| url | https://www.tandfonline.com/doi/10.1080/15476286.2023.2256578 |
| work_keys_str_mv | AT avasileva characterizationofcocas9nucleasefromcapnocytophagaochracea AT pselkova characterizationofcocas9nucleasefromcapnocytophagaochracea AT aarseniev characterizationofcocas9nucleasefromcapnocytophagaochracea AT mabramova characterizationofcocas9nucleasefromcapnocytophagaochracea AT nshcheglova characterizationofcocas9nucleasefromcapnocytophagaochracea AT omusharova characterizationofcocas9nucleasefromcapnocytophagaochracea AT imizgirev characterizationofcocas9nucleasefromcapnocytophagaochracea AT tartamonova characterizationofcocas9nucleasefromcapnocytophagaochracea AT mkhodorkovskii characterizationofcocas9nucleasefromcapnocytophagaochracea AT kseverinov characterizationofcocas9nucleasefromcapnocytophagaochracea AT ifedorova characterizationofcocas9nucleasefromcapnocytophagaochracea |