Near‐Freezing‐Temperature Golgi Neuronal Staining for X‐ray Imaging of Human Brain

Near‐Freezing‐Temperature Golgi Neuronal Staining for X‐ray Imaging of Human Brain

Abstract Achieving detailed neuronal structural information in large‐volume brain tissue has been a longstanding challenge in human brain imaging. A key obstacle arises from the trade‐off between staining efficiency and tissue autolysis. Traditional Golgi staining, typically conducted at room temper...

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Bibliographic Details
Main Authors:	Feng Zhou, Qiaowei Tang, Xin Yan, Chao Ma, Yu Zhang, Jichao Zhang, Qian Li, Lihua Wang, Jun Hu, Xiaoqing Cai, Jiang Li, Ying Zhu, Chunhai Fan
Format:	Article
Language:	English
Published:	Wiley 2025-08-01
Series:	Advanced Science
Subjects:	mercury chloride (HgCl2) near‐freezing‐temperature (NFT) Golgi neuron post‐mortem frozen human brain (PMF‐human brain) synchrotron‐based X‐ray microscopy
Online Access:	https://doi.org/10.1002/advs.202504468
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