Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity
Three forms of fruit are distinguished in the Oil palm (Elaeis guineensis Jacq.): Dura, Pisifera and Tenera. The most cultivated high yielding oil palm variety, the thin shell Tenera is produced from the cross between the thick shelled Dura and the shell-less Pisifera. Due to the perennial nature of...
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Cluj University Press
2017-12-01
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| Series: | Studia Universitatis Babeș-Bolyai. Biologia |
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| Online Access: | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/95 |
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| author | Vona D. ODENORE Geoffrey Obinna ANOLIEFO Beckley IKHAJIAGBE Omorefe ASEMOTA Chukwuemeka R. EKE Leonard O. IHASE |
| author_facet | Vona D. ODENORE Geoffrey Obinna ANOLIEFO Beckley IKHAJIAGBE Omorefe ASEMOTA Chukwuemeka R. EKE Leonard O. IHASE |
| author_sort | Vona D. ODENORE |
| collection | DOAJ |
| description | Three forms of fruit are distinguished in the Oil palm (Elaeis guineensis Jacq.): Dura, Pisifera and Tenera. The most cultivated high yielding oil palm variety, the thin shell Tenera is produced from the cross between the thick shelled Dura and the shell-less Pisifera. Due to the perennial nature of the oil palm, it is impossible to determine the fruit phenotype until it is reproductively mature. Oil palm farmers are therefore faced with the challenge of uncertainty of the kind of fruits and yield output expected until production time. The recent sequencing of the shell gene in the oil palm made it possible to identify Single Nucleotide Polymorphism (SNP) mutations that are now useful in developing molecular markers for fruit form predictions. In this study, Allele Specific PCR (AS-PCR) was used to assess regions of the shell gene containing SNPs for oil palm fruit form discrimination. Three primer pairs (S1, S2 and S3) were designed as common primers while four AS-PCR primers (S20, S22, S32 and S33) were adopted as inner primers. Possible combinations of common and AS primers were investigated in Dura, Pisifera and Tenera genomes. A 300bp fragment was observed to be unique in the Tenera shell gene when S3 was combined with S20 and S32. Other inner primer combinations investigated with S3 generated similar assays in Dura, Pisifera and Tenera. The result from this study detects a marker for the Tenera fruit form of the oil palm. |
| format | Article |
| id | doaj-art-08a2f39396224a5e8da430b37ef074bb |
| institution | DOAJ |
| issn | 2065-9512 |
| language | English |
| publishDate | 2017-12-01 |
| publisher | Cluj University Press |
| record_format | Article |
| series | Studia Universitatis Babeș-Bolyai. Biologia |
| spelling | doaj-art-08a2f39396224a5e8da430b37ef074bb2025-08-20T02:41:39ZengCluj University PressStudia Universitatis Babeș-Bolyai. Biologia2065-95122017-12-01334010.24193/subbbiol.2017.2.0395Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identityVona D. ODENORE0https://orcid.org/0000-0001-7259-0618Geoffrey Obinna ANOLIEFO1https://orcid.org/0000-0002-1130-382XBeckley IKHAJIAGBE2https://orcid.org/0000-0003-2834-7447Omorefe ASEMOTA3Chukwuemeka R. EKE4Leonard O. IHASE5University of Benin, Benin City, Nigeria. E-mail: beckley.ikhajiagbe@uniben.eduUniversity of Benin, Benin City, Nigeria. Email: geoffery.anoliefo@uniben.eduUniversity of Benin, Benin City, Nigeria. E-mail: beckley.ikhajiagbe@uniben.eduPhysiology Division, Nigerian Institute for Oil palm Research, Benin City, Nigeria. E-mail: beckley.ikhajiagbe@uniben.eduNigerian Institute for Oil palm Research, Benin City, Nigeria. E-mail: beckley.ikhajiagbe@uniben.eduNigerian Institute for Oil palm Research, Benin City, Nigeria. E-mail: beckley.ikhajiagbe@uniben.eduThree forms of fruit are distinguished in the Oil palm (Elaeis guineensis Jacq.): Dura, Pisifera and Tenera. The most cultivated high yielding oil palm variety, the thin shell Tenera is produced from the cross between the thick shelled Dura and the shell-less Pisifera. Due to the perennial nature of the oil palm, it is impossible to determine the fruit phenotype until it is reproductively mature. Oil palm farmers are therefore faced with the challenge of uncertainty of the kind of fruits and yield output expected until production time. The recent sequencing of the shell gene in the oil palm made it possible to identify Single Nucleotide Polymorphism (SNP) mutations that are now useful in developing molecular markers for fruit form predictions. In this study, Allele Specific PCR (AS-PCR) was used to assess regions of the shell gene containing SNPs for oil palm fruit form discrimination. Three primer pairs (S1, S2 and S3) were designed as common primers while four AS-PCR primers (S20, S22, S32 and S33) were adopted as inner primers. Possible combinations of common and AS primers were investigated in Dura, Pisifera and Tenera genomes. A 300bp fragment was observed to be unique in the Tenera shell gene when S3 was combined with S20 and S32. Other inner primer combinations investigated with S3 generated similar assays in Dura, Pisifera and Tenera. The result from this study detects a marker for the Tenera fruit form of the oil palm.https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/95allele specific pcr, dura, fruit forms, oil palm, pisifera, shell gene, tenera. |
| spellingShingle | Vona D. ODENORE Geoffrey Obinna ANOLIEFO Beckley IKHAJIAGBE Omorefe ASEMOTA Chukwuemeka R. EKE Leonard O. IHASE Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity Studia Universitatis Babeș-Bolyai. Biologia allele specific pcr, dura, fruit forms, oil palm, pisifera, shell gene, tenera. |
| title | Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity |
| title_full | Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity |
| title_fullStr | Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity |
| title_full_unstemmed | Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity |
| title_short | Development of a bidirectional PCR tool for Tenera oil palm (“Elaeis guineensis” Jacq.) identity |
| title_sort | development of a bidirectional pcr tool for tenera oil palm elaeis guineensis jacq identity |
| topic | allele specific pcr, dura, fruit forms, oil palm, pisifera, shell gene, tenera. |
| url | https://studiabiologia.reviste.ubbcluj.ro/index.php/studiabio/article/view/95 |
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