Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide

Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ–German Collection of Microorganisms and Cell...

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Main Authors: Imen Nouioui, Judith Boldt, Alina Zimmermann, Roman Makitrynskyy, Gabriele Pötter, Marlen Jando, Meike Döppner, Sarah Kirstein, Meina Neumann-Schaal, Juan Pablo Gomez-Escribano, Ulrich Nübel, Yvonne Mast
Format: Article
Language:English
Published: Elsevier 2024-01-01
Series:Current Research in Microbial Sciences
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Online Access:http://www.sciencedirect.com/science/article/pii/S2666517424000737
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author Imen Nouioui
Judith Boldt
Alina Zimmermann
Roman Makitrynskyy
Gabriele Pötter
Marlen Jando
Meike Döppner
Sarah Kirstein
Meina Neumann-Schaal
Juan Pablo Gomez-Escribano
Ulrich Nübel
Yvonne Mast
author_facet Imen Nouioui
Judith Boldt
Alina Zimmermann
Roman Makitrynskyy
Gabriele Pötter
Marlen Jando
Meike Döppner
Sarah Kirstein
Meina Neumann-Schaal
Juan Pablo Gomez-Escribano
Ulrich Nübel
Yvonne Mast
author_sort Imen Nouioui
collection DOAJ
description Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ–German Collection of Microorganisms and Cell Cultures decades ago. Genome-based phylogeny and in silico DNA-DNA hybridization supported the assignment of these strains to 26 novel species and two novel subspecies and a reclassification of a Streptomyces species. These results were consistent with the biochemical, enzymatic, and chemotaxonomic features of the strains. Most of the strains showed antimicrobial activities against a range of Gram-positive and Gram-negative bacteria, and against yeast. Genomic analysis revealed the presence of numerous unique biosynthetic gene clusters (BGCs) encoding for potential novel antibiotic and anti-cancer compounds. Strains DSM 41636T and DSM 61640T produced the antibiotic compounds A33853 and SF2768, respectively. Overall, this reflects the significant pharmaceutical and biotechnological potential of the proposed novel type strains and underlines the role of prokaryotic systematics for drug discovery. In order to compensate for the gender gap in naming prokaryotic species, we propose the eponyms for all newly described species to honour female scientists.
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spelling doaj-art-089c83aa6dc2439ab53bf6dea34871262025-08-20T02:19:55ZengElsevierCurrent Research in Microbial Sciences2666-51742024-01-01710029010.1016/j.crmicr.2024.100290Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwideImen Nouioui0Judith Boldt1Alina Zimmermann2Roman Makitrynskyy3Gabriele Pötter4Marlen Jando5Meike Döppner6Sarah Kirstein7Meina Neumann-Schaal8Juan Pablo Gomez-Escribano9Ulrich Nübel10Yvonne Mast11Leibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; Corresponding authors.Leibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; German Center for Infection Research (DZIF), Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; German Center for Infection Research (DZIF), Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; Braunschweig Integrated Centre of Systems Biology (BRICS), Rebenring 56, 38106 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany; Braunschweig Integrated Centre of Systems Biology (BRICS), Rebenring 56, 38106 Braunschweig, Germany; Technische Universität Braunschweig, Institut für Mikrobiologie, Rebenring 56, 38106 Braunschweig, GermanyLeibniz-Institut DSMZ – German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany; German Center for Infection Research (DZIF), Braunschweig, Germany; Braunschweig Integrated Centre of Systems Biology (BRICS), Rebenring 56, 38106 Braunschweig, Germany; Technische Universität Braunschweig, Institut für Mikrobiologie, Rebenring 56, 38106 Braunschweig, Germany; Corresponding authors.Actinomycetes are a prolific source of bioactive natural compounds many of which are used as antibiotics or other drugs. In this study we investigated the genomic and biochemical diversity of 32 actinobacterial strains that had been deposited at the DSMZ–German Collection of Microorganisms and Cell Cultures decades ago. Genome-based phylogeny and in silico DNA-DNA hybridization supported the assignment of these strains to 26 novel species and two novel subspecies and a reclassification of a Streptomyces species. These results were consistent with the biochemical, enzymatic, and chemotaxonomic features of the strains. Most of the strains showed antimicrobial activities against a range of Gram-positive and Gram-negative bacteria, and against yeast. Genomic analysis revealed the presence of numerous unique biosynthetic gene clusters (BGCs) encoding for potential novel antibiotic and anti-cancer compounds. Strains DSM 41636T and DSM 61640T produced the antibiotic compounds A33853 and SF2768, respectively. Overall, this reflects the significant pharmaceutical and biotechnological potential of the proposed novel type strains and underlines the role of prokaryotic systematics for drug discovery. In order to compensate for the gender gap in naming prokaryotic species, we propose the eponyms for all newly described species to honour female scientists.http://www.sciencedirect.com/science/article/pii/S2666517424000737ActinobacteriaSystematicsDrug discoveryAntibiotics
spellingShingle Imen Nouioui
Judith Boldt
Alina Zimmermann
Roman Makitrynskyy
Gabriele Pötter
Marlen Jando
Meike Döppner
Sarah Kirstein
Meina Neumann-Schaal
Juan Pablo Gomez-Escribano
Ulrich Nübel
Yvonne Mast
Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
Current Research in Microbial Sciences
Actinobacteria
Systematics
Drug discovery
Antibiotics
title Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
title_full Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
title_fullStr Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
title_full_unstemmed Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
title_short Biotechnological and pharmaceutical potential of twenty-eight novel type strains of Actinomycetes from different environments worldwide
title_sort biotechnological and pharmaceutical potential of twenty eight novel type strains of actinomycetes from different environments worldwide
topic Actinobacteria
Systematics
Drug discovery
Antibiotics
url http://www.sciencedirect.com/science/article/pii/S2666517424000737
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