24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors

Abstract The medial prefrontal cortex (mPFC) coordinates cognitive processes across multiple timescales, yet its role in multiple natural behaviors remain unclear. Existing neural recording methods are limited by short observation windows and artificial task constraints, preventing insights into ext...

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Main Authors: Jiaying Han, Fangxu Zhou, Zhong Zhao, Dong Zhang, Xueli Chen, Li Wang, Ting Zhao, Wei Ye, Xiaochun Gu, Huaqiang Fang, Jue Zhang, Lifeng Zhang, Heping Cheng
Format: Article
Language:English
Published: Nature Portfolio 2025-07-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05527-2
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author Jiaying Han
Fangxu Zhou
Zhong Zhao
Dong Zhang
Xueli Chen
Li Wang
Ting Zhao
Wei Ye
Xiaochun Gu
Huaqiang Fang
Jue Zhang
Lifeng Zhang
Heping Cheng
author_facet Jiaying Han
Fangxu Zhou
Zhong Zhao
Dong Zhang
Xueli Chen
Li Wang
Ting Zhao
Wei Ye
Xiaochun Gu
Huaqiang Fang
Jue Zhang
Lifeng Zhang
Heping Cheng
author_sort Jiaying Han
collection DOAJ
description Abstract The medial prefrontal cortex (mPFC) coordinates cognitive processes across multiple timescales, yet its role in multiple natural behaviors remain unclear. Existing neural recording methods are limited by short observation windows and artificial task constraints, preventing insights into extended, naturally relevant dynamics. Here, we present multimodal datasets integrating two-photon calcium imaging via miniature microscope, simultaneous behavior video recording, and continuous EEG-EMG recordings in freely moving mice for over 24 hours. These datasets capture uninterrupted neural activity (unconstrained calcium imaging), physiological rhythms, and naturalistic behaviors (12 classified states) across full circadian cycles. By bridging three critical gaps: long-term continuity (over 24 hours of recording), multiple spontaneous behavior (unrestrained freely moving mice), and multimodal integration (neural activity, brain states, and behavior), our work establishes an open-access benchmark for naturalistic neuroscience. These data enable investigations into how mPFC ensembles dynamically reconfigure across timescales, advancing computational models of cortical dynamics and offering new insights into experience-dependent plasticity and cross-modal interactions in real-world contexts.
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institution DOAJ
issn 2052-4463
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publishDate 2025-07-01
publisher Nature Portfolio
record_format Article
series Scientific Data
spelling doaj-art-089a1efca5434bebb3103ae207a502052025-08-20T03:04:18ZengNature PortfolioScientific Data2052-44632025-07-0112111210.1038/s41597-025-05527-224-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviorsJiaying Han0Fangxu Zhou1Zhong Zhao2Dong Zhang3Xueli Chen4Li Wang5Ting Zhao6Wei Ye7Xiaochun Gu8Huaqiang Fang9Jue Zhang10Lifeng Zhang11Heping Cheng12Academy for Advanced Interdisciplinary Studies, Peking UniversityCollege of Future Technology, Peking UniversityPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthAcademy for Advanced Interdisciplinary Studies, Peking UniversityPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthAcademy for Advanced Interdisciplinary Studies, Peking UniversityPKU-Nanjing Institute of Translational Medicine, Nanjing Raygen HealthAcademy for Advanced Interdisciplinary Studies, Peking UniversityAbstract The medial prefrontal cortex (mPFC) coordinates cognitive processes across multiple timescales, yet its role in multiple natural behaviors remain unclear. Existing neural recording methods are limited by short observation windows and artificial task constraints, preventing insights into extended, naturally relevant dynamics. Here, we present multimodal datasets integrating two-photon calcium imaging via miniature microscope, simultaneous behavior video recording, and continuous EEG-EMG recordings in freely moving mice for over 24 hours. These datasets capture uninterrupted neural activity (unconstrained calcium imaging), physiological rhythms, and naturalistic behaviors (12 classified states) across full circadian cycles. By bridging three critical gaps: long-term continuity (over 24 hours of recording), multiple spontaneous behavior (unrestrained freely moving mice), and multimodal integration (neural activity, brain states, and behavior), our work establishes an open-access benchmark for naturalistic neuroscience. These data enable investigations into how mPFC ensembles dynamically reconfigure across timescales, advancing computational models of cortical dynamics and offering new insights into experience-dependent plasticity and cross-modal interactions in real-world contexts.https://doi.org/10.1038/s41597-025-05527-2
spellingShingle Jiaying Han
Fangxu Zhou
Zhong Zhao
Dong Zhang
Xueli Chen
Li Wang
Ting Zhao
Wei Ye
Xiaochun Gu
Huaqiang Fang
Jue Zhang
Lifeng Zhang
Heping Cheng
24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
Scientific Data
title 24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
title_full 24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
title_fullStr 24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
title_full_unstemmed 24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
title_short 24-hour simultaneous mPFC’s miniature 2-photon imaging, EEG-EMG, and video recording during natural behaviors
title_sort 24 hour simultaneous mpfc s miniature 2 photon imaging eeg emg and video recording during natural behaviors
url https://doi.org/10.1038/s41597-025-05527-2
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