Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors
Diabetes mellitus type 1 is associated with an enhanced apoptosis of different cells and tissues, accelerating occurrence of diabetic microvascular complications. The aim of our study was to determine spontaneous apoptotic potential of the monocyte subsets in juvenile-onset complication-free diabete...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2014/946209 |
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| author | Jolanta Myśliwska Monika Ryba-Stanisławowska Marcin Smardzewski Bartosz Słomiński Małgorzata Myśliwiec Janusz Siebert |
| author_facet | Jolanta Myśliwska Monika Ryba-Stanisławowska Marcin Smardzewski Bartosz Słomiński Małgorzata Myśliwiec Janusz Siebert |
| author_sort | Jolanta Myśliwska |
| collection | DOAJ |
| description | Diabetes mellitus type 1 is associated with an enhanced apoptosis of different cells and tissues, accelerating occurrence of diabetic microvascular complications. The aim of our study was to determine spontaneous apoptotic potential of the monocyte subsets in juvenile-onset complication-free diabetes mellitus type 1 and to compare them with the corresponding values of the healthy. Moreover, we wanted to assess effects of TNF-R1 blocking agents and those of general TNF-α blocker (Infliximab) on spontaneous apoptosis of monocytes. Sixty randomly selected DM1 patients (14.5 ± 3.2 years) and 30 healthy (13.5 ± 2.8 years) volunteers were enrolled in the study. Our results indicate that three monocyte subsets are distinguishable in the groups of young diabetic patients and the healthy, similarly to in the blood of adults. DM1 patients were characterized by higher values of apoptotic monocytes than the healthy. The manipulation with drugs inhibiting TNF-R1 expression diminished the pool of CD16+ apoptotic monocytes. Infliximab reduced the apoptotic CD16− cells. In conclusion, diabetes mellitus type 1 is associated with greater apoptosis of three monocyte subsets which may contribute to the development of microvascular complications. TNF-α modifiers appear to ameliorate monocyte apoptosis. They may be useful for controlling excessive monocyte apoptosis in diabetic patients. |
| format | Article |
| id | doaj-art-08866c74067147ba9ad9b3b607adfa3b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-08866c74067147ba9ad9b3b607adfa3b2025-02-03T00:59:55ZengWileyMediators of Inflammation0962-93511466-18612014-01-01201410.1155/2014/946209946209Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α InhibitorsJolanta Myśliwska0Monika Ryba-Stanisławowska1Marcin Smardzewski2Bartosz Słomiński3Małgorzata Myśliwiec4Janusz Siebert5Department of Immunology, Medical University of Gdańsk, Ulica Dębinki 1, 80-211 Gdańsk, PolandDepartment of Immunology, Medical University of Gdańsk, Ulica Dębinki 1, 80-211 Gdańsk, PolandDepartment of Immunology, Medical University of Gdańsk, Ulica Dębinki 1, 80-211 Gdańsk, PolandDepartment of Immunology, Medical University of Gdańsk, Ulica Dębinki 1, 80-211 Gdańsk, PolandAcademic Clinic of Pediatrics, Hematology, Oncology and Endocrinology, Medical University of Gdańsk, Ulica Dębinki 7, 80-211 Gdańsk, PolandDepartment of Family Medicine, Medical University of Gdańsk, Ulica Dębinki 2, 80-211 Gdańsk, PolandDiabetes mellitus type 1 is associated with an enhanced apoptosis of different cells and tissues, accelerating occurrence of diabetic microvascular complications. The aim of our study was to determine spontaneous apoptotic potential of the monocyte subsets in juvenile-onset complication-free diabetes mellitus type 1 and to compare them with the corresponding values of the healthy. Moreover, we wanted to assess effects of TNF-R1 blocking agents and those of general TNF-α blocker (Infliximab) on spontaneous apoptosis of monocytes. Sixty randomly selected DM1 patients (14.5 ± 3.2 years) and 30 healthy (13.5 ± 2.8 years) volunteers were enrolled in the study. Our results indicate that three monocyte subsets are distinguishable in the groups of young diabetic patients and the healthy, similarly to in the blood of adults. DM1 patients were characterized by higher values of apoptotic monocytes than the healthy. The manipulation with drugs inhibiting TNF-R1 expression diminished the pool of CD16+ apoptotic monocytes. Infliximab reduced the apoptotic CD16− cells. In conclusion, diabetes mellitus type 1 is associated with greater apoptosis of three monocyte subsets which may contribute to the development of microvascular complications. TNF-α modifiers appear to ameliorate monocyte apoptosis. They may be useful for controlling excessive monocyte apoptosis in diabetic patients.http://dx.doi.org/10.1155/2014/946209 |
| spellingShingle | Jolanta Myśliwska Monika Ryba-Stanisławowska Marcin Smardzewski Bartosz Słomiński Małgorzata Myśliwiec Janusz Siebert Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors Mediators of Inflammation |
| title | Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors |
| title_full | Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors |
| title_fullStr | Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors |
| title_full_unstemmed | Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors |
| title_short | Enhanced Apoptosis of Monocytes from Complication-Free Juvenile-Onset Diabetes Mellitus Type 1 May Be Ameliorated by TNF-α Inhibitors |
| title_sort | enhanced apoptosis of monocytes from complication free juvenile onset diabetes mellitus type 1 may be ameliorated by tnf α inhibitors |
| url | http://dx.doi.org/10.1155/2014/946209 |
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