Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress
Abstract Polycystic Ovary Syndrome (PCOS) is a hormonal disorder affecting women of reproductive age, often associated with oxidative stress and inflammation. This study explores the therapeutic potential of Arctium lappa phenolic-rich fraction encapsulated nanofiber (ALPRF-NF) in a testosterone-ind...
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BMC
2025-05-01
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| Series: | Journal of Biological Engineering |
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| Online Access: | https://doi.org/10.1186/s13036-025-00515-x |
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| author | Viana Khojasteh Seyed Amirmohammad Mollanorouzi Ehsan Karimi Behrouz Ghorani Ehsan Oskoueian |
| author_facet | Viana Khojasteh Seyed Amirmohammad Mollanorouzi Ehsan Karimi Behrouz Ghorani Ehsan Oskoueian |
| author_sort | Viana Khojasteh |
| collection | DOAJ |
| description | Abstract Polycystic Ovary Syndrome (PCOS) is a hormonal disorder affecting women of reproductive age, often associated with oxidative stress and inflammation. This study explores the therapeutic potential of Arctium lappa phenolic-rich fraction encapsulated nanofiber (ALPRF-NF) in a testosterone-induced PCOS mouse model. All experiments were performed in triplicate and Duncan’s Multiple Range Test was used to assess significant differences between means, with significance determined at p < 0.05. The ALPRF-NF formulation demonstrated favorable physicochemical properties, including a ribbon-like structure (216.9 nm), a zeta potential of -19.3 mV, and a high encapsulation efficiency (93.1%). In vivo findings showed that ALPRF-NF significantly improved body weight, feed intake, and liver enzyme profiles in PCOS-induced mice (p ≤ 0.05). It also enhanced the antioxidant defense system by elevating levels of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT). Mechanistically, ALPRF-NF reduced oxidative stress and inflammation by delivering phenolic compounds that scavenge reactive oxygen species (ROS) and modulate gene expression in ovarian tissue. This included downregulation of inducible nitric oxide synthase (iNOS) and upregulation of SOD expression. These results suggest that ALPRF-NF effectively mitigates testosterone-induced ovarian oxidative damage and inflammation, offering a targeted, nanotechnology-based therapeutic approach for PCOS. The study provides valuable insights into novel strategies for improving women’s reproductive health through bioactive compound delivery. |
| format | Article |
| id | doaj-art-088213b35a0145f58f98048120b4df4e |
| institution | OA Journals |
| issn | 1754-1611 |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Biological Engineering |
| spelling | doaj-art-088213b35a0145f58f98048120b4df4e2025-08-20T01:49:44ZengBMCJournal of Biological Engineering1754-16112025-05-0119111010.1186/s13036-025-00515-xPhenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stressViana Khojasteh0Seyed Amirmohammad Mollanorouzi1Ehsan Karimi2Behrouz Ghorani3Ehsan Oskoueian4Department of Biology, Ma. C., Islamic Azad UniversityDepartment of Biology, Ma. C., Islamic Azad UniversityDepartment of Biology, Ma. C., Islamic Azad UniversityDepartment of Food Nanotechnology, Research Institute of Food Science and Technology (RIFST)Department of Biology, Ma. C., Islamic Azad UniversityAbstract Polycystic Ovary Syndrome (PCOS) is a hormonal disorder affecting women of reproductive age, often associated with oxidative stress and inflammation. This study explores the therapeutic potential of Arctium lappa phenolic-rich fraction encapsulated nanofiber (ALPRF-NF) in a testosterone-induced PCOS mouse model. All experiments were performed in triplicate and Duncan’s Multiple Range Test was used to assess significant differences between means, with significance determined at p < 0.05. The ALPRF-NF formulation demonstrated favorable physicochemical properties, including a ribbon-like structure (216.9 nm), a zeta potential of -19.3 mV, and a high encapsulation efficiency (93.1%). In vivo findings showed that ALPRF-NF significantly improved body weight, feed intake, and liver enzyme profiles in PCOS-induced mice (p ≤ 0.05). It also enhanced the antioxidant defense system by elevating levels of glutathione peroxidase (GPX), superoxide dismutase (SOD), and catalase (CAT). Mechanistically, ALPRF-NF reduced oxidative stress and inflammation by delivering phenolic compounds that scavenge reactive oxygen species (ROS) and modulate gene expression in ovarian tissue. This included downregulation of inducible nitric oxide synthase (iNOS) and upregulation of SOD expression. These results suggest that ALPRF-NF effectively mitigates testosterone-induced ovarian oxidative damage and inflammation, offering a targeted, nanotechnology-based therapeutic approach for PCOS. The study provides valuable insights into novel strategies for improving women’s reproductive health through bioactive compound delivery.https://doi.org/10.1186/s13036-025-00515-xNanomedicineDrug deliveryEncapsulationPolycystic ovary syndrome |
| spellingShingle | Viana Khojasteh Seyed Amirmohammad Mollanorouzi Ehsan Karimi Behrouz Ghorani Ehsan Oskoueian Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress Journal of Biological Engineering Nanomedicine Drug delivery Encapsulation Polycystic ovary syndrome |
| title | Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress |
| title_full | Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress |
| title_fullStr | Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress |
| title_full_unstemmed | Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress |
| title_short | Phenolic-loaded nanofiber from Arctium lappa root: a potential therapy for testosterone-induced ovarian oxidative stress |
| title_sort | phenolic loaded nanofiber from arctium lappa root a potential therapy for testosterone induced ovarian oxidative stress |
| topic | Nanomedicine Drug delivery Encapsulation Polycystic ovary syndrome |
| url | https://doi.org/10.1186/s13036-025-00515-x |
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