Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight.
<h4>Context</h4>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the mate...
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0085454 |
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| author | Charalambos Demetriou Sayeda Abu-Amero Anna C Thomas Miho Ishida Reena Aggarwal Lara Al-Olabi Lydia J Leon Jaime L Stafford Argyro Syngelaki Donald Peebles Kypros H Nicolaides Lesley Regan Philip Stanier Gudrun E Moore |
| author_facet | Charalambos Demetriou Sayeda Abu-Amero Anna C Thomas Miho Ishida Reena Aggarwal Lara Al-Olabi Lydia J Leon Jaime L Stafford Argyro Syngelaki Donald Peebles Kypros H Nicolaides Lesley Regan Philip Stanier Gudrun E Moore |
| author_sort | Charalambos Demetriou |
| collection | DOAJ |
| description | <h4>Context</h4>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.<h4>Objective</h4>The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.<h4>Design</h4>Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.<h4>Results</h4>Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).<h4>Interpretation</h4>Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth. |
| format | Article |
| id | doaj-art-08749f3843fc44aa87d216e6ca42c13d |
| institution | OA Journals |
| issn | 1932-6203 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-08749f3843fc44aa87d216e6ca42c13d2025-08-20T02:22:49ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0191e8545410.1371/journal.pone.0085454Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight.Charalambos DemetriouSayeda Abu-AmeroAnna C ThomasMiho IshidaReena AggarwalLara Al-OlabiLydia J LeonJaime L StaffordArgyro SyngelakiDonald PeeblesKypros H NicolaidesLesley ReganPhilip StanierGudrun E Moore<h4>Context</h4>Fetal growth involves highly complex molecular pathways. IGF2 is a key paternally expressed growth hormone that is critical for in utero growth in mice. Its role in human fetal growth has remained ambiguous, as it has only been studied in term tissues. Conversely the maternally expressed growth suppressor, PHLDA2, has a significant negative correlation between its term placental expression and birth weight.<h4>Objective</h4>The aim of this study is to address the role in early gestation of expression of IGF1, IGF2, their receptors IGF1R and IGF2R, and PHLDA2 on term birth weight.<h4>Design</h4>Real-time quantitative PCR was used to investigate mRNA expression of IGF1, IGF2, IGF1R, IGF2R and PHLDA2 in chorionic villus samples (CVS) (n = 260) collected at 11-13 weeks' gestation. Expression was correlated with term birth weight using statistical package R including correction for several confounding factors.<h4>Results</h4>Transcript levels of IGF2 and IGF2R revealed a significant positive correlation with birth weight (0.009 and 0.04, respectively). No effect was observed for IGF1, IGF1R or PHLDA2 and birth weight. Critically, small for gestational age (SGA) neonates had significantly lower IGF2 levels than appropriate for gestational age neonates (p = 3.6 × 10(-7)).<h4>Interpretation</h4>Our findings show that IGF2 mRNA levels at 12 weeks gestation could provide a useful predictor of future fetal growth to term, potentially predicting SGA babies. SGA babies are known to be at a higher risk for type 2 diabetes. This research reveals an imprinted, parentally driven rheostat for in utero growth.https://doi.org/10.1371/journal.pone.0085454 |
| spellingShingle | Charalambos Demetriou Sayeda Abu-Amero Anna C Thomas Miho Ishida Reena Aggarwal Lara Al-Olabi Lydia J Leon Jaime L Stafford Argyro Syngelaki Donald Peebles Kypros H Nicolaides Lesley Regan Philip Stanier Gudrun E Moore Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. PLoS ONE |
| title | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. |
| title_full | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. |
| title_fullStr | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. |
| title_full_unstemmed | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. |
| title_short | Paternally expressed, imprinted insulin-like growth factor-2 in chorionic villi correlates significantly with birth weight. |
| title_sort | paternally expressed imprinted insulin like growth factor 2 in chorionic villi correlates significantly with birth weight |
| url | https://doi.org/10.1371/journal.pone.0085454 |
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