Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil
Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs)....
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The Journal of Infection in Developing Countries
2015-09-01
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| Series: | Journal of Infection in Developing Countries |
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| author | André Victor Barbosa Aloysio de Mello Figueiredo Cerqueira Leonardo Alves Rusak Cristhiane Moura Falavina dos Reis Nilma Cintra Leal Ernesto Hofer Deyse Christina Vallim |
| author_facet | André Victor Barbosa Aloysio de Mello Figueiredo Cerqueira Leonardo Alves Rusak Cristhiane Moura Falavina dos Reis Nilma Cintra Leal Ernesto Hofer Deyse Christina Vallim |
| author_sort | André Victor Barbosa |
| collection | DOAJ |
| description | Introduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs).
Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis.
Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECII-positive; and two ECI strains from a human case (1982) and from bovine meat (2009).
Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings.
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| format | Article |
| id | doaj-art-086e4c34fe9f4e6aa805835398f67bb8 |
| institution | Kabale University |
| issn | 1972-2680 |
| language | English |
| publishDate | 2015-09-01 |
| publisher | The Journal of Infection in Developing Countries |
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| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-086e4c34fe9f4e6aa805835398f67bb82025-08-20T03:48:47ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802015-09-0190910.3855/jidc.5639Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in BrazilAndré Victor Barbosa0Aloysio de Mello Figueiredo Cerqueira1Leonardo Alves Rusak2Cristhiane Moura Falavina dos Reis3Nilma Cintra Leal4Ernesto Hofer5Deyse Christina Vallim6Universidade Federal Fluminense, Niterói, Rio de Janeiro, BrazilUniversidade Federal Fluminense, Niterói, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Rio de Janeiro, BrazilCentro de Pesquisas Aggeu Magalhães, Fiocruz, Recife, Pernambuco, BrazilInstituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Rio de Janeiro, BrazilInstituto Oswaldo Cruz, Fiocruz, Rio de Janeiro, Rio de Janeiro, BrazilIntroduction: Listeria monocytogenes is an important foodborne pathogen and the 4b serotype is responsible for many cases of human listeriosis reported in Brazil. Several listeriosis outbreaks worldwide have involved a small number of well-defined clonal groups, designated as epidemic clones (ECs). Methodology: We studied 71 strains of serotype 4b, including 25 isolates from human cases of listeriosis and 46 from meat-based foods, collected in Brazil between 1977 and 2010. The presence of ECs (I and II) markers and virulence genes (inlA, inlB, ilnC, inlJ and actA) were evaluated by PCR assay. The genetic relationship of ECs-positive strains was assessed by pulsed field gel electrophoresis. Results: ECI and ECII markers were found both in human and food strains, with 19.7% positive for the ECI marker and 40.8% for ECII. Most strains (97.2%) were positive for the virulence genes that were studied. Nevertheless, the actA gene amplicons showed two distinct sizes, with all ECI positive strains exhibiting a 105bp deletion. Pulsed field gel electrophoresis (PFGE) analysis allowed the recognition of highly related strains, particularly from two outbreaks of neonatal listeriosis in São Paulo State occurred in 1992 and 1997, both ECII-positive; and two ECI strains from a human case (1982) and from bovine meat (2009). Conclusions: The presence of ECs among clinical samples and beef isolates of serotype 4b from some regions of Brazil highlights the need for rigorous control of production procedures. Furthermore, the association of ECII with two nosocomial outbreaks suggests its ability to spread in these settings. https://jidc.org/index.php/journal/article/view/5639Listeria monocytogenesserotype 4bepidemic clonesvirulenceepidemiology |
| spellingShingle | André Victor Barbosa Aloysio de Mello Figueiredo Cerqueira Leonardo Alves Rusak Cristhiane Moura Falavina dos Reis Nilma Cintra Leal Ernesto Hofer Deyse Christina Vallim Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil Journal of Infection in Developing Countries Listeria monocytogenes serotype 4b epidemic clones virulence epidemiology |
| title | Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil |
| title_full | Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil |
| title_fullStr | Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil |
| title_full_unstemmed | Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil |
| title_short | Characterization of epidemic clones of Listeria monocytogenes serotype 4b isolated from humans and meat products in Brazil |
| title_sort | characterization of epidemic clones of listeria monocytogenes serotype 4b isolated from humans and meat products in brazil |
| topic | Listeria monocytogenes serotype 4b epidemic clones virulence epidemiology |
| url | https://jidc.org/index.php/journal/article/view/5639 |
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