Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor
We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immo...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Electrochemistry |
| Online Access: | http://dx.doi.org/10.4061/2011/603257 |
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| author | H. Baccar T. Ktari A. Abdelghani |
| author_facet | H. Baccar T. Ktari A. Abdelghani |
| author_sort | H. Baccar |
| collection | DOAJ |
| description | We present a comparison between two biosensors for hydrogen peroxide (H2O2) detection.
The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor
was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated
with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors
using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio
attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM. |
| format | Article |
| id | doaj-art-0866f1cdea4b4e89997d6aaf3e80d03b |
| institution | Kabale University |
| issn | 2090-3537 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Electrochemistry |
| spelling | doaj-art-0866f1cdea4b4e89997d6aaf3e80d03b2025-02-03T07:24:52ZengWileyInternational Journal of Electrochemistry2090-35372011-01-01201110.4061/2011/603257603257Functionalized Palladium Nanoparticles for Hydrogen Peroxide BiosensorH. Baccar0T. Ktari1A. Abdelghani2University of Carthage, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, BP 676, 1080 Charguia Cedex, TunisiaUniversity of Carthage, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, BP 676, 1080 Charguia Cedex, TunisiaUniversity of Carthage, Nanotechnology Laboratory, National Institute of Applied Science and Technology, Centre Urbain Nord, BP 676, 1080 Charguia Cedex, TunisiaWe present a comparison between two biosensors for hydrogen peroxide (H2O2) detection. The first biosensor was developed by the immobilization of Horseradish Peroxidase (HRP) enzyme on thiol-modified gold electrode. The second biosensor was developed by the immobilization of cysteamine functionalizing palladium nanoparticles on modified gold surface. The amino groups can be activated with glutaraldehyde for horseradish peroxidase immobilization. The detection of hydrogen peroxide was successfully observed in PBS for both biosensors using the cyclic voltammetry and the chronoamperometry techniques. The results show that the limit detection depends on the large surface-to-volume ratio attained with palladium nanoparticles. The second biosensor presents a better detection limit of 7.5 μM in comparison with the first one which is equal to 75 μM.http://dx.doi.org/10.4061/2011/603257 |
| spellingShingle | H. Baccar T. Ktari A. Abdelghani Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor International Journal of Electrochemistry |
| title | Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor |
| title_full | Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor |
| title_fullStr | Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor |
| title_full_unstemmed | Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor |
| title_short | Functionalized Palladium Nanoparticles for Hydrogen Peroxide Biosensor |
| title_sort | functionalized palladium nanoparticles for hydrogen peroxide biosensor |
| url | http://dx.doi.org/10.4061/2011/603257 |
| work_keys_str_mv | AT hbaccar functionalizedpalladiumnanoparticlesforhydrogenperoxidebiosensor AT tktari functionalizedpalladiumnanoparticlesforhydrogenperoxidebiosensor AT aabdelghani functionalizedpalladiumnanoparticlesforhydrogenperoxidebiosensor |